Recombinant MERS-CoV 3CL Protease, CF

Catalog #: E-719 Datasheet

Catalog #	Availability	Size / Price	Qty
E-719-050

R&D Systems Recombinant Proteins and Enzymes

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All 3CL Protease Proteins and Enzymes

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Recombinant MERS-CoV 3CL Protease, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial MERS-CoV 3CL Protease concentration of 50-100 nM when using SARS CoV-2 3CL Protease Substrate, Rh110-conjugated (Catalog # S-720).

Source

E. coli-derived mers-cov 3CL Protease protein
Ser1 - Gln306 (Val304Leu & Met305Phe) with a C-terminal 6-His tag

Accession #

YP_009047217.1

Predicted Molecular Mass

33 kDa

Product Datasheets

E-719

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-719

Formulation	Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Background: 3CL Protease

The 3CL protease (aka 3CL^pro, M^pro or "Main" Protease) from the human MERS-CoV coronavirus (Middle East Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide that cleaves proteins with sequences including a LQ[S/A/G] c-terminal to the glutamine residue. 3CL protease activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.

References