Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF

Catalog #: AVI11508 Datasheet

Fc Chimera, biotinylated

Catalog #	Availability	Size / Price	Qty
AVI11508-100

Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein Binding Activity.

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All VCAM-1/CD106 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VCAM-1/CD106 Fc Avi-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human VCAM 1/CD106 Fc Chimera Avi-tag (Catalog # AVI11508) binds Recombinant Human Integrin alpha 4 beta 1 (Catalog # 5668-A4) with an ED₅₀ of 7.50-90.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein

Human VCAM-1 (Phe25-Glu698) Accession # P19320.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P19320.1

N-terminal Sequence
Analysis

Phe25

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

103 kDa

SDS-PAGE

105-130 kDa, under reducing conditions.

Product Datasheets

AVI11508

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11508

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) binds Recombinant Human Integrin alpha 4 beta 1 (5668-A4) with an ED₅₀ of 7.50‑90.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human VCAM‑1/CD106 Fc Chimera Avi-tag Protein (Catalog # AVI11508) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-130 kDa, and 210-260 kDa, respectively.

Background: VCAM-1/CD106

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. Our Avi-tag Biotinylated human VCAM-1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1