Recombinant Human Tyro3/Dtk His-tag Protein, CF Summary
Product Specifications
Ala41-Ser428, with a C-terminal 6-His tag
Analysis
Product Datasheets
10365-DK
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10365-DK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Tyro3/Dtk His-tag Protein (Catalog # 10365-DK) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Gas6 (Catalog # 885-GSB) binds with an ED50 of 0.075-0.45 µg/mL.
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2 μg/lane of Recombinant Human Tyro3/Dtk His-tag (Catalog # 10365-DK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-71 kDa.
Background: Tyro3/Dtk
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily (1, 2). The overall genomic structure of Dtk is virtually identical to that determined for the human UFO gene. This particular genomic organization is likely to have been duplicated and closely maintained throughout evolution (3). The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases (1). All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anti-coagulation factor protein S (1, 2). The binding affinities for Gas6 is in the order of Axl > Dtk > Mer (1). Gas6 binding induces tyrosine phosphorylation and down-stream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (1, 2). Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain (4). The full length of Human DTK is 428 amino acids, with 387 amino acid signal sequence in the mature extracellular domain. The ECD of human DTK has 7 glycosylation sites. Within the ECD, human DTK shares 85% sequence identity with both mouse and rat DTK.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
- Paula, M. et al. (1996) Genomics 31:13.
- Philip, S. et al. (1994) Growth Factor 11:125.
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