Recombinant Human TREM-1 His-tag Protein, CF Summary
Product Specifications
Ala21-Arg200, with a C-terminal 6-His tag
Analysis
Product Datasheets
10337-TR
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10337-TR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human PGLYRP1/PGRP-S Protein (Catalog # 2590-PGB) is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human TREM-1 His-tag (Catalog # 10337-TR) that produces 50% of the optimal binding response is found to be approximately 50-300 ng/mL.
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2 μg/lane of Recombinant Human TREM-1 His-tag (Catalog # 10337-TR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-45 kDa.
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells), also known as CD354, along with TREM-2, and rodent specific TREM-3, comprise a group of Ig superfamily proteins regulating the activation and differentiation of myeloid cells. Human TREM-1 is type I transmembrane protein containing a single Ig-like domain in the extracellular domain (ECD), a transmembrane region and a short cytoplasmic tail. The mature ECD of human TREM-1 shares 45% identity with mouse TREM-1. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1, expressed on monocytes and neutrophils, associates with the adapter protein, DAP12, to deliver an activating signal to elicit and amplify the innate inflammatory response triggered by bacteria (1, 2). A few potential TREM-1 ligands have been identified, including HMGB1 and PGLYRP1 (1-2). HMGB1 (high mobility group Box 1), a ubiquitous nuclear protein, is secreted by myeloid cells during inflammation and has been suggested as an additional TREM-1 ligand (1). However, HMGB1 alone has been found unable to trigger TREM-1 activation and may require co-activating molecules (1). PGLYRP1 (peptidoglycan recognition receptor 1), mainly found in granulocytes, binds to peptidoglycan and cell wall components such as LPS, and has been identified as another potential TREM-1 ligand (1, 2). PGLYRP1, when complexed with PGN, is able to activate TREM-1 and enhance cytokine production in human neutrophils and macrophages (2).
- Tammaro, et al. (2017) Pharmacology & Therapeutics. 177:81.
- Read, et al. (2015) J Immunol. 194:1417.
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