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Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Catalog #: 10140-T2
3 Citations Datasheet
Catalog # Availability Size / Price Qty
10140-T2-100
Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity
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Product Details
Citations (3)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.75-6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein
Human TRAIL R2/TNFRSF10B
(Ala54-Ser210)
Accession # AAH01281.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
AAH01281.1
N-terminal Sequence
Analysis
Ala54
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa
SDS-PAGE
54-61 kDa, under reducing conditions

Product Datasheets

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10140-T2

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10140-T2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Inhibition Activity Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein Inhibition Activity View Larger

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 0.75-6 ng/mL.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).

References
  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 2
Entrez Gene IDs
8795 (Human); 21933 (Mouse)
Alternate Names
CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma
    Authors: AV Yagolovich, AA Isakova, AA Artykov, YV Vorontsova, DV Mazur, NV Antipova, MS Pavlyukov, MI Shakhparon, AM Gileva, EA Markvichev, EA Plotnikova, AA Pankratov, MP Kirpichnik, ME Gasparian, DA Dolgikh
    International Journal of Molecular Sciences, 2022-10-21;23(20):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Dual role of ERK2/NF-kappaB signaling in TRAIL sensitivity
    Authors: MW Lee, DS Kim, JE Eom, JW Lee, KW Sung, HH Koo, YB Hong, KH Yoo
    American journal of cancer research, 2022-07-15;12(7):3373-3389.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  3. TRAILshort Protects against CD4 T Cell Death during Acute HIV Infection
    Authors: S Natesampil, AC Paim, NW Cummins, AP Chandrasek, GD Bren, SR Lewin, HP Kiem, AD Badley
    J. Immunol., 2019-06-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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