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Recombinant Human TIM-3 Fc Chimera Protein, CF

Catalog #: 2365-TM
11 Citations 3 Reviews Datasheet
Catalog # Availability Size / Price Qty
2365-TM-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (11)
FAQs
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Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TIM-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin‑9 (Catalog # 2045-GA) at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera with an apparent Kd <30 nM.
Source
Mouse myeloma cell line, NS0-derived human TIM-3 protein
Human TIM-3
(Ser22-Arg200)
Accession # Q8TDQ0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q8TDQ0
N-terminal Sequence
Analysis
Ser22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46.4 kDa (monomer)
SDS-PAGE
66 kDa, reducing conditions

Product Datasheets

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2365-TM

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2365-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References
  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Monney, L. et al. (2002) Nature 415:536.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

Citations for Recombinant Human TIM-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. TIM-3 drives temporal differences in restimulation-induced cell death sensitivity in effector CD8+ T cells in conjunction with CEACAM1
    Authors: CM Lake, K Voss, BM Bauman, K Pohida, T Jiang, G Dveksler, AL Snow
    Cell Death & Disease, 2021-04-14;12(4):400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble TIM3 and Its Ligands Galectin-9 and CEACAM1 Are in Disequilibrium During Alcohol-Related Liver Disease and Promote Impairment of Anti-bacterial Immunity
    Authors: A Riva, E Palma, D Devshi, D Corrigall, H Adams, N Heaton, K Menon, M Preziosi, A Zamalloa, R Miquel, JM Ryan, G Wright, S Fairclough, A Evans, D Shawcross, R Schierwage, S Klein, FE Uschner, M Praktiknjo, K Katzarov, T Hadzhiolov, S Pavlova, M Simonova, J Trebicka, R Williams, S Chokshi
    Frontiers in Physiology, 2021-03-10;12(0):632502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Dietary, nondigestible oligosaccharides and Bifidobacterium breve M-16V suppress allergic inflammation in intestine via targeting dendritic cell maturation
    Authors: S de Kivit, AI Kostadinov, J Kerperien, ME Morgan, VA Muruzabal, GA Hofman, LMJ Knippels, AD Kraneveld, J Garssen, LEM Willemsen
    J. Leukoc. Biol., 2017-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Tim-3 protects decidual stromal cells from toll-like receptor-mediated apoptosis and inflammatory reactions and promotes Th2 bias at the maternal-fetal interface.
    Authors: Wang S, Cao C, Piao H, Li Y, Tao Y, Zhang X, Zhang D, Sun C, Zhu R, Wang Y, Yuan M, Li D, Du M
    2015-03-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014-09-24;9(9):e107920.
    Species: Bacteria, Human
    Sample Types: Protein, Whole Cells
    Applications: Enzyme Assay, Enzyme Assay Substrate
  6. Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line.
    Authors: Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, Nakae S
    PLoS ONE, 2014-01-20;9(1):e86106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013-03-21;9(3):e1003253.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012-10-24;7(10):e47648.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B.
    Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
    Eur. J. Immunol., 2012-05-01;42(5):1180-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
    Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
    PLoS ONE, 2011-03-11;6(3):e17787.
    Species: Human
    Sample Types: Buffer
    Applications: Binding Assay
  11. Dysregulation of TIM-3-Galectin-9 Pathway in the Cystic Fibrosis Airways.
    Authors: Vega-Carrascal I, Reeves EP, Niki T, Arikawa T, McNally P, O'Neill SJ, Hirashima M, McElvaney NG
    J. Immunol., 2011-01-24;0(0):.
    Species: Human
    Sample Types: BALF, Whole Cells
    Applications: Bioassay, Enzyme Assay

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Reviews for Recombinant Human TIM-3 Fc Chimera Protein, CF

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Recombinant Human TIM-3 Fc Chimera Protein, CF
By Anonymous on 11/13/2019
Application: Binding assay/Protein-protein interaction
Recombinant Human TIM-3 Fc Chimera Protein, CF
By Anonymous on 08/22/2018
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human TIM-3 Fc Chimera Protein, CF 2365-TM
Recombinant Human TIM-3 Fc Chimera Protein, CF
By Shelley Anderson on 10/20/2017
Application: Apoptosis assay