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Recombinant Human Tie-2 Fc Chimera Protein, CF

Catalog #: 313-TI
14 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
313-TI-100
Recombinant Human Tie-2 Fc Chimera Protein Binding Activity
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Tie-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin‑2 (Catalog # 623-AN) binds with an ED50 of 0.5‑4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Tie-2 protein
Human Tie-2
(Ala23-Lys745)
Accession # Q02763		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
Q02763
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
100 kDa (monomer)
SDS-PAGE
135 kDa, reducing conditions

Product Datasheets

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313-TI

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

313-TI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Tie-2 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds with an ED50 of 0.5-4 ng/mL.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

Citations for Recombinant Human Tie-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1
    Authors: Y Koya, H Nara, S Yagi, C Ueno, M Kamohara
    Scientific Reports, 2021-07-07;11(1):14021.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  2. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
    Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
    Elife, 2018-11-16;7(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA, Surface Plasmon Resonance
  3. Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy
    Authors: T Shlamkovic, L Aharon, WA Barton, N Papo
    Oncotarget, 2017-05-16;8(20):33571-33585.
    Species: Yeast - Saccharomyces cerevisiae
    Sample Types: Protein
    Applications: Bioassay
  4. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells
    Authors: Wilson Chan
    PLoS ONE, 2016-06-15;11(6):e0156994.
    Species: Human
    Sample Types: Protein
    Applications: ELISA (Standard)
  5. Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis.
    Authors: Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, Bai Y
    PLoS ONE, 2014-02-19;9(2):e89148.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression.
    Authors: Buchanan A, Clementel V, Woods R, Harn N, Bowen M, Mo W, Popovic B, Bishop S, Dall'Acqua W, Minter R, Jermutus L, Bedian V
    MAbs, 2013-02-14;5(2):255-62.
    Species: Human
    Sample Types: Protein
    Applications: Functional ELISA
  7. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
    Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
    Nat. Cell Biol., 2008-04-20;10(5):527-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
    Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
    Mol. Biol. Cell, 2006-10-11;17(12):5163-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation.
    Authors: Red-Horse K, Rivera J, Schanz A, Zhou Y, Winn V, Kapidzic M, Maltepe E, Okazaki K, Kochman R, Vo KC, Giudice L, Erlebacher A, McCune JM, Stoddart CA, Fisher SJ
    J. Clin. Invest., 2006-09-21;116(10):2643-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent.
    Authors: Zhu T, Sennlaub F, Beauchamp MH, Fan L, Joyal JS, Checchin D, Nim S, Lachapelle P, Sirinyan M, Hou X, Bossolasco M, Rivard GE, Heveker N, Chemtob S
    Arterioscler. Thromb. Vasc. Biol., 2006-01-26;26(4):744-50.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody.
    Authors: Popkov M, Jendreyko N, McGavern DB, Rader C, Barbas CF
    Cancer Res., 2005-02-01;65(3):972-81.
    Applications: Binding Assay
  12. Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2.
    Authors: Oliner J, Min H, Leal J, Yu D, Rao S, You E, Tang X, Kim H, Meyer S, Han SJ, Hawkins N, Rosenfeld R, Davy E, Graham K, Jacobsen F, Stevenson S, Ho J, Chen Q, Hartmann T, Michaels M, Kelley M, Li L, Sitney K, Martin F, Sun JR, Zhang N, Lu J, Estrada J, Kumar R, Coxon A, Kaufman S, Pretorius J, Scully S, Cattley R, Payton M, Coats S, Nguyen L, Desilva B, Ndifor A, Hayward I, Radinsky R, Boone T, Kendall R
    Cancer Cell, 2004-11-01;6(5):507-16.
    Species: Human
    Sample Types:
    Applications: Functional ELISA
  13. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004-03-01;15(3):566-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Applications: ELISA (Standard)

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Recombinant Human Tie-2 Fc Chimera Protein, CF
By Anonymous on 08/04/2019
Application: Binding assay/Protein-protein interaction
Reason for Rating: This Recombinant Human Tie-2 Fc protein is very pure and can bind to Dynabeads with Protein G very well.
Binding assay/Protein-protein interaction Recombinant Human Tie-2 Fc Chimera Protein, CF 313-TI