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Recombinant Human Thrombopoietin/TPO Protein, CF

Catalog #: BT-TPO
1 Citation Datasheet
Catalog # Availability Size / Price Qty
BT-TPO-010
BT-TPO-050
BT-TPO-100
BT-TPO-250
BT-TPO-500
BT-TPO-01M
Equivalent Bioactivity of GMP,Animal-Free, and RUO gradesof Recombinant HumanThrombopoietin/Tpo proteins.
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Product Details
Citations (1)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Thrombopoietin/TPO Protein, CF Summary

  • R&D Systems E. coli-derived Recombinant Human Thrombopoietin/TPO Protein (BT-TPO)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.0500-0.500 ng/mL.
Source
E. coli-derived human Thrombopoietin/Tpo protein
Ser22-Leu195
Accession #
NP_000451.1
N-terminal Sequence
Analysis
Ala-Ser22
Predicted Molecular Mass
19 kDa
SDS-PAGE
18-19 kDa, under reducing conditions.

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BT-TPO

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-TPO

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. 
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Equivalent bioactivity of GMP(BT-TPO-GMP),Animal-Free (BT-TPO-AFL) and RUO (Catalog # BT-TPO) grades of RecombinantHuman Thrombopoietin/Tpoproteins as measured in a cellproliferation assay using MO7ehuman megakaryocytic leukemiccells (orange, green, red,respectively).

Bioactivity View Larger

Recombinant Human Thrombopoietin/Tpo (Catalog # BT-TPO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.0500‑0.500 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Thrombopoietin/Tpo Protein (Catalog # BT-TPO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-19 kDa, under reducing conditions.

Background: Thrombopoietin/Tpo

Thrombopoietin (TPO) is a crucial regulator of hematopoietic stem cell (HSC) differentiation, maturation, and proliferation, as well as megakaryocytopoiesis and thrombopoiesis. TPO is often used in conjunction with Stem Cell Factor (SCF) and Flt-3 ligand in cell culture protocols to expand HSCs for bone marrow transplantation and cellular therapies. When combined with SCF and Flt-3 ligand, it promotes the differentiation of HSCs into megakaryocytes, leading to the production of platelets. It has shown promise in emerging cellular therapies for treating sickle cell disease, beta thalassemia, and other blood-related disorders. Moreover, TPO in combination with other cytokines is utilized in the generation of iNK cells, which have the potential to be incorporated into cellular immunotherapies.

Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

Citation for Recombinant Human Thrombopoietin/TPO Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. High frequency CCR5 editing in human hematopoietic stem progenitor cells protects xenograft mice from HIV infection
    Authors: Claiborne, D;Detwiler, Z;Docken, S;Borland, T;Cromer, D;Simkhovich, A;Ophinni, Y;Okawa, K;Bateson, T;Chen, T;Hudson, W;Trifonova, R;Davenport, M;Ho, T;Boutwell, C;Allen, T;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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