Home / TGF-beta 1 / Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Catalog #: 7754-BH
68 Citations 15 Reviews Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
7754-BH-005/CF
7754-BH-025/CF
7754-BH-100/CF
7754-BH-500/CF
7754-BH-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
7754-BH-005
7754-BH-025
7754-BH-100
7754-BH-500
7754-BH-01M
Graph showing dose-dependent bioactivity of Human TGF-beta 1 protein
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Product Details
Citations (68)
FAQs
Reviews (15)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Ala279-Ser390
Accession #
P01137
N-terminal Sequence
Analysis
Ala279
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
11 kDa, reducing conditions

Product Datasheets

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7754-BH (with carrier)

Product Datasheet

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7754-BH/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7754-BH

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7754-BH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing dose-dependent bioactivity of Human TGF-beta 1 protein View Larger

Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Background: TGF-beta 1

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 1
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

68 Citations: Showing 1 - 10
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  1. Tissue-specific functions of MSCs are linked to homeostatic muscle maintenance and alter with aging
    Authors: Kurosawa, T;Ikemoto-Uezumi, M;Yoshimoto, Y;Minato, K;Kaji, N;Chaen, T;Hase, E;Minamikawa, T;Yasui, T;Horiguchi, K;Iino, S;Hori, M;Uezumi, A;
    Aging cell
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. In vitro evidence for the potential of EGFR inhibitors to decrease the TGF-?1-induced dispersal of circulating tumour cell clusters mediated by EGFR overexpression
    Authors: Hapeman, JD;Galwa, R;Carneiro, CS;Nedelcu, AM;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TGF-?1 maintains the developmental potential of embryonic submandibular gland epithelia separated with mesenchyme
    Authors: Li, H;Wang, G;Zhao, G;Liu, H;Liu, L;Cao, Y;Li, C;
    Heliyon
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Interferon subverts an AHR-JUN axis to promote CXCL13+ T cells in lupus
    Authors: Law, C;Wacleche, VS;Cao, Y;Pillai, A;Sowerby, J;Hancock, B;Horisberger, A;Bracero, S;Skidanova, V;Li, Z;Adejoorin, I;Dillon, E;Benque, IJ;Nunez, DP;Simmons, DP;Keegan, J;Chen, L;Baker, T;Brohawn, PZ;Al-Mossawi, H;Hao, LY;Jones, B;Rao, N;Qu, Y;Alves, SE;Accelerating Medicines Partnership: RA/SLE Network, ;Jonsson, AH;Shaw, KS;Vleugels, RA;Massarotti, E;Costenbader, KH;Brenner, MB;Lederer, JA;Hultquist, JF;Choi, J;Rao, DA;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Identification of a distal enhancer regulating hedgehog interacting protein gene in human lung epithelial cells
    Authors: Guo, F;Zhang, L;Yu, Y;Gong, L;Tao, S;Werder, RB;Mishra, S;Zhou, Y;Anamika, WJ;Lao, T;Inuzuka, H;Zhang, Y;Pham, B;Liu, T;Tufenkjian, TS;Richmond, BW;Wei, W;Mou, H;Wilson, AA;Hu, M;Chen, W;Zhou, X;
    EBioMedicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Targeting HDAC6 to treat heart failure with preserved ejection fraction in mice
    Authors: Ranjbarvaziri, S;Zeng, A;Wu, I;Greer-Short, A;Farshidfar, F;Budan, A;Xu, E;Shenwai, R;Kozubov, M;Li, C;Van Pell, M;Grafton, F;MacKay, CE;Song, X;Priest, JR;Argast, G;Mandegar, MA;Hoey, T;Yang, J;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Epidermal growth factor receptor activation is essential for kidney fibrosis development
    Authors: Cao, S;Pan, Y;Terker, AS;Arroyo Ornelas, JP;Wang, Y;Tang, J;Niu, A;Kar, SA;Jiang, M;Luo, W;Dong, X;Fan, X;Wang, S;Wilson, MH;Fogo, A;Zhang, MZ;Harris, RC;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. TIM-3 Expression and M2 Polarization of Macrophages in the TGF?-Activated Tumor Microenvironment in Colorectal Cancer
    Authors: Katagata, M;Okayama, H;Nakajima, S;Saito, K;Sato, T;Sakuma, M;Fukai, S;Endo, E;Sakamoto, W;Saito, M;Saze, Z;Momma, T;Mimura, K;Kono, K;
    Cancers
    Applications: Cell Culture
  9. A model for the dissemination of circulating tumour cell clusters involving platelet recruitment and a plastic switch between cooperative and individual behaviours
    Authors: Hapeman, JD;Carneiro, CS;Nedelcu, AM;
    BMC ecology and evolution
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Treprostinil Reconstitutes Mitochondrial Organisation and Structure in Idiopathic Pulmonary Fibrosis Cells
    Authors: Fang, L;Chen, WC;Jaksch, P;Molino, A;Saglia, A;Roth, M;Lambers, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Senescence-Driven Inflammatory and Trophic Microenvironment Imprints Mesenchymal Stromal/Stem Cells in Osteoarthritic Patients
    Authors: Fusi, G;Constantinides, M;Fissoun, C;Pichard, L;Pers, YM;Ferreira-Lopez, R;Pantesco, V;Poulet, C;Malaise, O;De Seny, D;Lemaitre, JM;Jorgensen, C;Brondello, JM;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Cancer-associated mesothelial cells are regulated by the anti-Müllerian hormone axis
    Authors: Chauvin, M;Meinsohn, MC;Dasari, S;May, P;Iyer, S;Nguyen, NMP;Oliva, E;Lucchini, Z;Nagykery, N;Kashiwagi, A;Mishra, R;Maser, R;Wells, J;Bult, CJ;Mitra, AK;Donahoe, PK;Pépin, D;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Organ function is preserved despite reorganization of niche architecture in the hair follicle
    Authors: Wei, H;Du, S;Parksong, J;Pasolli, HA;Matte-Martone, C;Regot, S;Gonzalez, LE;Xin, T;Greco, V;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Polymer-Functionalized Mitochondrial Transplantation to Fibroblasts Counteracts a Pro-Fibrotic Phenotype
    Authors: Baudo, G;Wu, S;Massaro, M;Liu, H;Lee, H;Zhang, A;Hamilton, DJ;Blanco, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. TGF? signaling links early-life endocrine-disrupting chemicals exposure to suppression of nucleotide excision repair in rat myometrial stem cells
    Authors: Bariani, MV;Cui, YH;Ali, M;Bai, T;Grimm, SL;Coarfa, C;Walker, CL;He, YY;Yang, Q;Al-Hendy, A;
    Research square
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches
    Authors: De Martin, A;Stanossek, Y;L�tge, M;Cadosch, N;Onder, L;Cheng, HW;Brandstadter, JD;Maillard, I;Stoeckli, SJ;Pikor, NB;Ludewig, B;
    Nature immunology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. TGF-beta is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells
    Authors: V Klück, G Cab?u, L Mies, F Bukkems, L van Emst, R Bakker, A van Caam, HINT conso, TO Cri?an, LAB Joosten
    Arthritis Research & Therapy, 2023-02-27;25(1):30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. 5-FU-miR-15a Inhibits Activation of Pancreatic Stellate Cells by Reducing YAP1 and BCL-2 Levels In Vitro
    Authors: VMD Almanzar, K Shah, JF LaComb, A Mojumdar, HR Patel, J Cheung, M Tang, J Ju, AB Bialkowska
    International Journal of Molecular Sciences, 2023-02-16;24(4):.
    Species: Human, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Nsun2 coupling with RoRgammat shapes the fate of Th17 cells and promotes colitis
    Authors: WL Yang, W Qiu, T Zhang, K Xu, ZJ Gu, Y Zhou, HJ Xu, ZZ Yang, B Shen, YL Zhao, Q Zhou, Y Yang, W Li, PY Yang, YG Yang
    Nature Communications, 2023-02-16;14(1):863.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation
    Authors: DI Cho, MJ Ahn, HH Cho, M Cho, JH Jun, BG Kang, SY Lim, SJ Yoo, MR Kim, HS Kim, SJ Lee, LT Dat, C Lee, YS Kim, Y Ahn
    Experimental & Molecular Medicine, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. DRAK2 contributes to type 1 diabetes by negatively regulating IL-2 sensitivity to alter regulatory T�cell development
    Authors: AH Mandarano, TL Harris, BM Creasy, M Wehenkel, M Duggar, BA Wilander, A Mishra, JC Crawford, SA Mullen, KM Williams, M Pillai, AA High, MA McGargill
    Cell Reports, 2023-02-10;42(2):112106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. xcore: an R package for inference of gene expression regulators
    Authors: M Migda?, T Arakawa, S Takizawa, M Furuno, H Suzuki, E Arner, CL Winata, B Kaczkowski
    BMC Bioinformatics, 2023-01-11;24(1):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. TGF-beta signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development
    Authors: J Feng, X Han, Y Yuan, CK Cho, E Jane?ková, T Guo, S Pareek, MS Rahman, B Zheng, J Bi, J Jing, M Zhang, J Xu, TV Ho, Y Chai
    Elife, 2022-12-21;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  24. Poricoic acid A suppresses renal fibroblast activation and interstitial fibrosis in UUO rats via upregulating Sirt3 and promoting beta-catenin K49 deacetylation
    Authors: DQ Chen, L Chen, Y Guo, XQ Wu, TT Zhao, HL Zhao, HJ Zhang, MH Yan, GQ Zhang, P Li
    Acta pharmacologica Sinica, 2022-12-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Nanoparticle endothelial delivery of PGC-1alpha attenuates hypoxia-induced pulmonary hypertension by attenuating EndoMT-caused vascular wall remodeling
    Authors: D Cai, SY Chen
    Redox Biology, 2022-10-28;58(0):102524.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
    Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
    Front Immunol, 2022-09-29;13(0):933329.
    Species: Complex Species Category
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis
    Authors: B Jaeger, JC Schupp, L Plappert, O Terwolbeck, N Artysh, G Kayser, P Engelhard, TS Adams, R Zweigerdt, H Kempf, S Lienenklau, W Garrels, I Nazarenko, D Jonigk, M Wygrecka, D Klatt, A Schambach, N Kaminski, A Prasse
    Nature Communications, 2022-09-26;13(1):5637.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  28. LINC01146/F11R facilitates growth and metastasis of prostate cancer under the regulation of TGF-beta
    Authors: X Guo, Y Gu, C Guo, L Pei, C Hao
    The Journal of steroid biochemistry and molecular biology, 2022-09-23;0(0):106193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  29. HDAC5-mediated Smad7 silencing through MEF2A is critical for fibroblast activation and hypertrophic scar formation
    Authors: Y Gao, Y Liu, D Zheng, C Ho, D Wen, J Sun, L Huang, Y Liu, Q Li, Y Zhang
    International journal of biological sciences, 2022-09-11;18(15):5724-5739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 2022-08-29;23(17):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  31. Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha
    Authors: Z Cai, S Tian, T Klein, L Tu, LW Geenen, T Koudstaal, AE van den Bo, YB de Rijke, IKM Reiss, E Boersma, C van der Le, M Van Faasse, I Kema, DJ Duncker, KA Boomars, K Tran-Lundm, C Guignabert, D Merkus
    Scientific Reports, 2022-07-19;12(1):12326.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. SMAD3 mutation in LDS3 causes bone fragility by impairing the TGF-beta pathway and enhancing osteoclastogenesis
    Authors: A El-Gazzar, H Kang, N Fratzl-Zel, E Webb, AM Barnes, M Jovanovic, SG Mehta, V Datta, V Saraff, RK Dale, F Rauch, JC Marini, W Högler
    Bone Reports, 2022-07-16;17(0):101603.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells
    Authors: RB Werder, T Liu, KM Abo, J Lindstrom-, C Villacorta, J Huang, A Hinds, N Boyer, E Bullitt, M Liesa, EK Silverman, DN Kotton, MH Cho, X Zhou, AA Wilson
    Oncogene, 2022-07-13;8(28):eabo6566.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Hypo-osmotic stress induces the epithelial alarmin IL-33 in the colonic barrier of ulcerative colitis
    Authors: MD Gundersen, KB Larsen, KM Johnsen, R Goll, J Florholmen, G Haraldsen
    Scientific Reports, 2022-07-07;12(1):11550.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  35. Sphingosine kinase 1 mediates sexual dimorphism in fibrosis in a mouse model of NASH.
    Authors: Montefusco D, Jamil M, Maczis M, Schroeder W, Levi M, Ranjit S, Allegood J, Bandyopadhyay D, Retnam R, Spiegel S, Cowart L
    Mol Metab, 2022-06-06;62(0):101523.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Modified Shenlingbaizhu Decoction represses the pluripotency of colorectal cancer stem cells by inhibiting TGF-beta mediated EMT program.
    Authors: Dai Y, Wang H, Sun R, Diao J, Ma Y, Shao M, Xu Y, Zhang Q, Gao Z, Zeng Z, Zhang L, Sun X
    Phytomedicine, 2022-06-01;103(0):154234.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Fibrotic Signaling in Cardiac Fibroblasts and Vascular Smooth Muscle Cells: The Dual Roles of Fibrosis in HFpEF and CAD
    Authors: JC Bachmann, SJ Baumgart, AK Uryga, MH Bosteen, G Borghetti, M Nyberg, KM Herum
    Cells, 2022-05-17;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Proximal tubular RAGE mediated the renal fibrosis in UUO model mice via upregulation of autophagy
    Authors: B Liu, T Sun, H Li, S Qiu, Y Li, D Zhang
    Cell Death & Disease, 2022-04-23;13(4):399.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Dedifferentiation of Human Cardiac Myofibroblasts Is Independent of Activation of COX-2/PGE2 Pathway
    Authors: VT Luu, S Phan, ZQ Jin
    International Journal of Molecular Sciences, 2022-03-11;23(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Identifying Function Determining Residues in Neuroimmune Semaphorin 4A
    Authors: SP Chapoval, M Lee, A Lemmer, O Ajayi, X Qi, AF Neuwald, AD Keegan
    International Journal of Molecular Sciences, 2022-03-11;23(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Down-regulation of the endothelial histone demethylase JMJD3 is associated with neointimal hyperplasia of arteriovenous fistulas in kidney failure
    Authors: S Feng, EK Peden, Q Guo, TH Lee, Q Li, Y Yuan, C Chen, F Huang, J Cheng
    The Journal of Biological Chemistry, 2022-03-10;0(0):101816.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. PD-L1 mediates lung fibroblast to myofibroblast transition through Smad3 and beta-catenin signaling pathways
    Authors: X Guo, C Sunil, O Adeyanju, A Parker, S Huang, M Ikebe, TA Tucker, S Idell, G Qian
    Scientific Reports, 2022-02-23;12(1):3053.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-beta receptor I versus II
    Authors: L Sun, Y Wang, X Wang, A Navarro-Co, S Ilyas, N Jalan-Sakr, C Gan, X Tu, Y Shi, K Tu, Q Liu, Z Lou, H Dong, AH Sharpe, VH Shah, N Kang
    Cell Reports, 2022-02-08;38(6):110349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Quantifying Cell-Derived Changes in Collagen Synthesis, Alignment, and Mechanics in a 3D Connective Tissue Model.
    Authors: Wilks B, Evans E, Howes A, Hopkins C, Nakhla M, Williams G, Morgan J
    Adv Sci (Weinh), 2022-02-01;9(10):e2103939.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis via regulating Smurf 2/Smad 7.
    Authors: Liu Y, Guo W, Guo Y, Chen X, Liu W
    Front Biosci (Landmark Ed), 2022-01-12;27(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Spatiotemporal control of myofibroblast activation in acoustically-responsive scaffolds via ultrasound-induced matrix stiffening.
    Authors: Farrell E, Aliabouzar M, Quesada C, Baker B, Franceschi R, Putnam A, Fabiilli M
    Acta Biomater, 2021-11-20;138(0):133-143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids
    Authors: T Suezawa, S Kanagaki, K Moriguchi, A Masui, K Nakao, M Toyomoto, K Tamai, R Mikawa, T Hirai, K Murakami, M Hagiwara, S Gotoh
    Stem Cell Reports, 2021-11-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Lobeglitazone, A Peroxisome Proliferator-Activated Receptor-Gamma Agonist, Inhibits Papillary Thyroid Cancer Cell Migration and Invasion by Suppressing p38 MAPK Signaling Pathway.
    Authors: Jin J, Han J, Ha J, Baek H, Lim D
    Endocrinol Metab (Seoul), 2021-10-14;36(5):1095-1110.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Monoacylglycerol lipase deficiency in the tumor microenvironment slows tumor growth in non-small cell lung cancer.
    Authors: Kienzl M, Hasenoehrl C, Maitz K, Sarsembayeva A, Taschler U, Valadez-Cosmes P, Kindler O, Ristic D, Raftopoulou S, Santiso A, Barnthaler T, Brcic L, Hahnefeld L, Gurke R, Thomas D, Geisslinger G, Kargl J, Schicho R
    Oncoimmunology, 2021-09-11;10(1):1965319.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Ventral stress fibers induce plasma membrane deformation in human fibroblasts.
    Authors: Ghilardi S, Aronson M, Sgro A
    Mol Biol Cell, 2021-06-30;32(18):1707-1723.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  51. Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 Patients.
    Authors: Marwick J, Elliott R, Longden J, Makda A, Hirani N, Dhaliwal K, Dawson J, Carragher N
    SLAS Discov, 2021-06-02;26(9):1091-1106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. The Conversion of Human Tissue-Like Inflammatory Monocytes Into Macrophages.
    Authors: Bsat M, Mehta H, Rubio M, Sarfati M
    Curr Protoc, 2021-03-01;1(3):e74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Identification of metabolism-associated genes and construction of a prognostic signature in bladder cancer
    Authors: C Shen, J Liu, L Wang, Z Liang, H Niu, Y Wang
    Cancer Cell International, 2020-11-04;20(1):538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Tumor Cell-Derived TGFbeta1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA.
    Authors: Wu P, Geng B, Chen Q, Zhao E, Liu J, Sun C, Zha C, Shao Y, You B, Zhang W, Li L, Meng X, Cai J, Li X
    Cancer Immunol Res, 2020-09-30;8(12):1470-1484.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Lack of evidence supporting a role of IFN-&beta and TGF-&beta in differential polarization of Bordetella pertussis specific-T cell responses
    Authors: R da Silva A, LG Quiambao, F Soldevila, A Sutherland, B Peters, A Sette
    Cytokine, 2020-09-29;137(0):155313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. MAPK activity dynamics regulate non-cell autonomous effects of oncogene expression
    Authors: TJ Aikin, AF Peterson, MJ Pokrass, HR Clark, S Regot
    Elife, 2020-09-17;9(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Nucleolar protein NOP2/NSUN1 suppresses HIV-1 transcription and promotes viral latency by competing with Tat for TAR binding and methylation
    Authors: W Kong, A Biswas, D Zhou, G Fiches, K Fujinaga, N Santoso, J Zhu
    PLoS Pathog., 2020-03-16;16(3):e1008430.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  58. CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro
    Authors: S Zhou, SD da Silva, PM Siegel, A Philip
    Sci Rep, 2019-11-06;9(1):16317.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Accumulation of versican facilitates wound healing: Implication of its initial ADAMTS-cleavage site.
    Authors: Islam S, Chuensirikulchai K, Khummuang S, Keratibumrungpong T, Kongtawelert P, Kasinrerk W, Hatano S, Nagamachi A, Honda H, Watanabe H
    Matrix Biol, 2019-10-26;87(0):77-93.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. ERK Regulates HIF1alpha-Mediated Platinum Resistance by Directly Targeting PHD2 in Ovarian Cancer.
    Authors: Li Z, Zhou W, Zhang Y, Sun W, Yung M, Sun J, Li J, Chen C, Li Z, Meng Y, Chai J, Zhou Y, Liu S, Cheung A, Ngan H, Chan D, Zheng W, Zhu W
    Clin Cancer Res, 2019-07-08;25(19):5947-5960.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Ruxolitinib in combination with prednisone and nilotinib exhibit synergistic effects in human cells lines and primary cells from myeloproliferative neoplasms
    Authors: A Arenas Cor, R Ayala Diaz, P Hernández-, J Gorrochate, D Primo, A Robles, ML Morales, J Ballestero, I Rapado, M Gallardo, M Linares, J Martínez-L
    Haematologica, 2018-12-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Retinoic acid signaling is essential for airway smooth muscle homeostasis
    Authors: F Chen, F Shao, A Hinds, S Yao, S Ram-Mohan, TA Norman, R Krishnan, A Fine
    JCI Insight, 2018-08-23;3(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Normal breast-derived epithelial cells with luminal and intrinsic subtype-enriched gene expression document inter-individual differences in their differentiation cascade
    Authors: B Kumar, MS Prasad, P Bhat-Naksh, M Anjanappa, M Kalra, N Marino, AMV Storniolo, X Rao, S Liu, J Wan, Y Liu, H Nakshatri
    Cancer Res., 2018-07-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Posttranscriptional Regulation of LOXL1 Expression Via Alternative Splicing and Nonsense-Mediated mRNA Decay as an Adaptive Stress Response
    Authors: D Berner, M Zenkel, F Pasutto, U Hoja, P Liravi, GC Gusek-Schn, FE Kruse, J Schödel, A Reis, U Schlötzer-
    Invest. Ophthalmol. Vis. Sci., 2017-11-01;58(13):5930-5940.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
    Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
    Front Microbiol, 2017-06-07;8(0):1035.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Decoding NADPH oxidase 4 expression in human tumors
    Authors: JL Meitzler, HR Makhlouf, S Antony, Y Wu, D Butcher, G Jiang, A Juhasz, J Lu, I Dahan, P Jansen-Dür, H Pircher, AM Shah, K Roy, JH Doroshow
    Redox Biol, 2017-05-26;13(0):182-195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. The human RNA surveillance factor Up-frameshift 1 inhibits hepatic cancer progression by targeting MRP2/ABCC2.
    Authors: Zhang H, You Y, Zhu Z
    Biomed Pharmacother, 2017-05-26;92(0):365-372.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Glucocorticoids Have Opposing Effects on Liver Fibrosis in Hepatic Stellate and Immune Cells.
    Authors: Kim K, Lee J, Zhou Y, Harpavat S, Moore D
    Mol Endocrinol, 2016-06-29;30(8):905-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    • TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  2. Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

    • Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

  3. Has the half-life of recombinant human TGF beta 1 been determined?

    • While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Average Rating: 4.8 (Based on 15 Reviews)

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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 09/07/2023
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: We used 10nM of TGF-beta to treat quiescent cancer associated fibroblasts, to transform them into activated cancer associated fibroblasts, as an positive control.
Stem/Immune cell maintenance or differentiation Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/02/2023
Application: Apoptosis assay
Apoptosis assay Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 01/20/2023
Application: CellProlif
Reason for Rating: I used it in a number of cells in several assays and always worked as expected.
Cell Proliferation Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 11/18/2020
Application: In vitro bioactivity in cell culture
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Yuqing Feng on 10/17/2019
Application: In vitro bioactivity in cell culture
Reason for Rating: No discernible difference between HEK293-derived TGFb vs CHO-derived TGFb (cat#240-B) was found
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 08/30/2019
Application: In vitro bioactivity in cell culture
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Jenna Nguyen on 05/07/2019
Application: Binding assay/Protein-protein interaction
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/10/2019
Application: Immunoassay Standard
Immunoassay Standard Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 05/22/2018
Application: CellProlif
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 04/12/2018
Application: Cell migration/motility
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 03/08/2018
Application: CellProlif
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 03/07/2018
Application: Enzymatic activity in vitro
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/17/2018
Application: Apoptosis assay
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 02/14/2018
Application: CellProlif
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
By Anonymous on 10/01/2017
Application: Stem/Immune cell maintenance or differentiation
Stem/Immune cell maintenance or differentiation Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH