Recombinant Human TGF-beta 1 Biotinylated Protein, CF

Catalog #: BT7754 Datasheet

Human Cell-expressed, Biotinylated

Catalog #	Availability	Size / Price	Qty
BT7754-025

Recombinant Human TGF-beta 1 Biotinylated Protein Binding Activity.

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All TGF-beta 1 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TGF-beta 1 Biotinylated Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human TGF-beta RII Fc Chimera (Catalog # 341-BR) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human TGF-beta 1 (Catalog # BT7754) binds with an ED₅₀ of 1.50-15.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Ala279-Ser390

Accession #

P01137.2

N-terminal Sequence
Analysis

Ala279

Structure / Form

Disulfide-linked homodimer

Biotinylated via amines

Predicted Molecular Mass

12.8 kDa

SDS-PAGE

8-14 kDa, under reducing conditions.

Product Datasheets

BT7754

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT7754

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in 4mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human TGF‑ beta RII Fc Chimera (341-BR) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human TGF‑ beta 1 (Catalog # BT7754) binds with an ED₅₀ of 1.50-15.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TGF-beta 1 Biotinylated Protein (Catalog # BT7754) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-14 kDa and 20-25 kDa, respectively.

Background: TGF-beta 1

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑beta 1, 2 and 3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑beta 1 shares 100% aa identity with pig, dog and cow TGF‑beta 1, and 99% aa identity with mouse, rat and horse TGF‑beta 1. It demonstrates cross‑species activity (1). TGF‑beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑beta in different contexts (11).

References

Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
Wahl, S.M. (2006) Immunol. Rev. 213:213.
Chang, H. et al. (2002) Endocr. Rev. 23:787.
Lin, J.S. et al. (2006) Reproduction 132:179.
Hinck, A.P. et al. (1996) Biochemistry 35:8517.
Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
Derynck, R. et al. (1985) Nature 316:701.
Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Alternate Names

CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1