Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF
Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF Summary
Product Specifications
Gln31-Gln152 (pro domain), Ser153-Gln692 (mature form) with a C-terminal 6-His and Avitag
Analysis
Product Datasheets
AVI3888
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI3888
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human LDLR (2148-LD/CF) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human Proprotein Convertase 9/PCSK9 His-tag Avi-tag Protein (Catalog # AVI3888) binds with an ED50 of 0.150-1.20 µg/mL.
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2 μg/lane of Biotinylated Recombinant Human Proprotein Convertase 9/PCSK9 His-tag Avi-tag Protein (Catalog # ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-21 kDa & 62-69 kDa, under reducing conditions.
Background: Proprotein Convertase 9/PCSK9
PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain (2). Although the other members of the proprotein convertase family demonstrate activity on several downstream targets, PCSK9 protease activity has only been demonstrated through its autocatalytic processing (3). PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (4-7). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (8-10). These reported functions create significant interest in PCSK9 as a pharmacological target in cardiovascular disease (11). PCSK9 has been reported to interact with non-LDR targets as well, including mediators of inflammation and immunological processes (12).
- Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
- Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
- Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
- Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
- DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
- Canuel, M. et al. (2013) PLoS One 8:e64145.
- Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
- Levy, E. et al. (2013) Atherosclerosis 227:297.
- Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
- Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
- Chen, B. et al. (2019) Curr.Top Med. Chem. 19:1790.
- Dixon, D.L. et al. (2016) J. Clin. Lipidol. 10:1073.
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