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Recombinant Human PDGF R beta Fc Chimera Protein

Catalog #: 385-PR
13 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
385-PR-100/CF

With Carrier

Catalog # Availability Size / Price Qty
385-PR-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (13)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PDGF R beta Fc Chimera Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1-3 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) .
Source
Mouse myeloma cell line, NS0-derived human PDGF R beta protein
Human PDGF R beta
Leu33-Phe530
(Glu241Asp)
Accession # P09619		 ENIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P09619
N-terminal Sequence
Analysis
Leu33
Predicted Molecular Mass
84 kDa (monomer)
SDS-PAGE
94-124 kDa, reducing conditions

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385-PR (with carrier)

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385-PR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

385-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

385-PR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,
PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of
five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and
hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

Citations for Recombinant Human PDGF R beta Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Decreased functional connectivity is associated with increased levels of Cerebral Spinal Fluid soluble-PDGFRbeta, a marker of blood brain barrier breakdown, in older adults
    Authors: JA Contreras, K Fujisaki, N Ortega, G Barisano, A Sagare, I Pappas, H Chui, JM Ringman, EB Joe, B Zlokovic, AW Toga, J Pa
    Research square, 2023-03-07;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  2. DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously
    Authors: R Beckmann, K Jensen, S Fenn, J Speck, K Krause, A Meier, M Röth, S Fauser, R Kimbung, DT Logan, M Steegmaier, H Kettenberg
    Nature Communications, 2021-01-29;12(1):708.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  3. Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology
    Authors: J Park, KS Gill, AA Aghajani, JD Heredia, H Choi, A Oberstein, E Procko
    PLoS Pathog., 2020-06-19;16(6):e1008647.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
    Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
    Sci Rep, 2017-11-27;7(1):16439.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  5. A novel excision selection method for isolation of antibodies binding antigens expressed specifically by rare cells in tissue sections
    Authors: S Lykkemark, OA Mandrup, MB Jensen, J Just, P Kristensen
    Nucleic Acids Res., 2017-06-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  6. Targeted Delivery to Tumor-associated Pericytes via an Affibody with High Affinity for PDGFR? Enhances the in vivo Antitumor Effects of Human TRAIL
    Authors: Z Tao, H Yang, Q Shi, Q Fan, L Wan, X Lu
    Theranostics, 2017-06-01;7(8):2261-2276.
    Applications: Bioassay
  7. A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells
    Authors: C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger
    PLoS Pathog., 2017-04-12;13(4):e1006273.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
    Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
    PLoS Pathog., 2017-04-12;13(4):e1006281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Generation and characterization of ABBV642, a dual variable domain immunoglobulin molecule (DVD-Ig) that potently neutralizes VEGF and PDGF-BB and is designed for the treatment of exudative age-related macular degeneration
    Authors: Kun Ding
    MAbs, 2017-02-01;0(0):0.
    Applications: Bioassay
  10. Platelet-derived growth factor receptor-beta antagonism restores morphine analgesic potency against neuropathic pain.
    Authors: Donica C, Cui Y, Shi S, Gutstein H
    PLoS ONE, 2014-05-12;9(5):e97105.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  11. Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer.
    Authors: Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L
    Mol Ther, 2014-01-02;22(4):828-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
    Br. J. Cancer, 2012-10-09;107(10):1702-13.
    Applications: Bioassay
  13. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
    Authors: Lacoste E, Martineau I, Gagnon G
    J. Periodontol., 2003-10-01;74(10):1498-507.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Capture)

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