Recombinant Human PDGF-BB Protein, CF Summary
Product Specifications
Ser82-Thr190
Analysis
Product Datasheets
220-BB
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
220-BB
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Background: PDGF-BB
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
Citations for Recombinant Human PDGF-BB Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Branched chain ?-ketoacids aerobically activate HIF1? signaling in vascular cells
Authors: Xiao, W;Shrimali, N;Oldham, WM;Clish, CB;He, H;Wong, SJ;Wertheim, BM;Arons, E;Haigis, MC;Leopold, JA;Loscalzo, J;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor
Authors: Guo, T;Pei, F;Zhang, M;Yamada, T;Feng, J;Jing, J;Ho, TV;Chai, Y;
Cell stem cell
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
C5a-C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms
Authors: Okada, A;Shimizu, K;Kawashima, A;Kayahara, T;Itani, M;Kurita, H;Miyamoto, S;Kataoka, H;Aoki, T;
Scientific reports
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Antifibrotic effects of vitamin D3 on human lung fibroblasts derived from patients with idiopathic pulmonary fibrosis
Authors: Voisin, T;Joannes, A;Morzadec, C;Lagadic-Gossmann, D;Naoures, CL;De Latour, BR;Rouze, S;Jouneau, S;Vernhet, L;
The Journal of nutritional biochemistry
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Fibroblast growth factor 8b (FGF-8b) enhances myogenesis and inhibits adipogenesis in rotator cuff muscle cell populations in vitro
Authors: Otsuka, T;Kan, HM;Mengsteab, PY;Tyson, B;Laurencin, CT;
Proceedings of the National Academy of Sciences of the United States of America
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats
Authors: Li, X;Quan, H;He, J;Li, H;Zhu, Q;Wang, Y;Zhu, Y;Ge, RS;
The Journal of steroid biochemistry and molecular biology
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
SRF SUMOylation modulates smooth muscle phenotypic switch and vascular remodeling
Authors: Xu, Y;Zhang, H;Pober, J;Min, W;Zhou, H;
Research square
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Identification of cell-biologic mechanisms of coronary artery spasm and its ex vivo diagnosis using peripheral blood-derived iPSCs
Authors: HM Yang, JE Lee, JY Kim, J You, J Kim, HS Lee, HM Yoo, MG Kong, JK Han, HJ Cho, KW Park, HJ Kang, BK Koo, YB Park, HS Kim
Biomaterials research, 2023-02-18;27(1):16.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cancer cell-derived novel periostin isoform promotes invasion in head and neck squamous cell carcinoma
Authors: S Wenhua, T Tsunematsu, M Umeda, H Tawara, N Fujiwara, Y Mouri, R Arakaki, N Ishimaru, Y Kudo
Cancer Medicine, 2023-01-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The extract from Hyssopus cuspidatus Boriss. Prevents bronchial airway remodeling by inhibiting mouse bronchial wall thickening and hASMC proliferation and migration
Authors: X Cai, Y Mao, X Shen, H Li, J He, M Zhang
Journal of ethnopharmacology, 2022-12-14;0(0):116047.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeting endogenous kidney regeneration using anti-IL11 therapy in acute and chronic models of kidney disease
Authors: AA Widjaja, S Viswanatha, SG Shekeran, E Adami, WW Lim, S Chothani, J Tan, JWT Goh, HM Chen, SY Lim, CM Boustany-K, J Hawkins, E Petretto, N Hübner, S Schafer, TM Coffman, SA Cook
Nature Communications, 2022-12-05;13(1):7497.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth
Authors: S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland
JCI Insight, 2022-11-08;7(21):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of Functional Immortalized Human Corneal Stromal Stem Cells
Authors: A Dos Santos, N Lyu, A Balayan, R Knight, KS Zhuo, Y Sun, J Xu, ML Funderburg, JL Funderburg, SX Deng
International Journal of Molecular Sciences, 2022-11-02;23(21):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion
Authors: W He, X Zhu, A Xin, H Zhang, Y Sun, H Xu, H Li, T Yang, D Zhou, H Yan, X Sun
Cell & bioscience, 2022-10-18;12(1):175.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis
Authors: A Filliol, Y Saito, A Nair, DH Dapito, LX Yu, A Ravichandr, S Bhattachar, S Affo, N Fujiwara, H Su, Q Sun, TM Savage, JR Wilson-Kan, JM Caviglia, L Chin, D Chen, X Wang, S Caruso, JK Kang, AD Amin, S Wallace, R Dobie, D Yin, OM Rodriguez-, C Yin, A Mehal, B Izar, RA Friedman, RG Wells, UB Pajvani, Y Hoshida, HE Remotti, N Arpaia, J Zucman-Ros, M Karin, NC Henderson, I Tabas, RF Schwabe
Nature, 2022-10-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
Oncology Letters, 2022-09-30;24(5):417.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism
Authors: R Chandrasek, C Mathieu, R Sheth, AP Cheng, D Fong, R McCormack, H El-Gabalaw, S Alishetty, M Paige, CD Hoemann
PLoS ONE, 2022-09-15;17(9):e0274420.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Increased expression and accumulation of GDF15 in IPF extracellular matrix contribute to fibrosis
Authors: A Radwanska, CT Cottage, A Piras, C Overed-Say, C Sihlbom, R Budida, C Wrench, J Connor, S Monkley, P Hazon, H Schluter, MJ Thomas, CM Hogaboam, LA Murray
JCI Insight, 2022-08-22;7(16):.
Species: Mouse
Sample Types: Whole Cells
Applications: Migration Bioassay -
Peficitinib inhibits fibroblast-like synoviocyte activation and angiogenic vascular endothelial tube formation via inhibitory effects on PDGF and VEGF signaling in addition to JAK
Authors: G Ishikawa, C Kwon, Y Fujii
Journal of pharmacological sciences, 2022-07-31;150(2):74-80.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1alpha-dependent expression of collagen I (alpha2) during renal fibrosis
Authors: F Das, N Ghosh-Chou, S Maity, BS Kasinath, GG Choudhury
The Journal of Biological Chemistry, 2022-07-11;0(0):102246.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Kir4.2 Potassium Channels in Retinal Pigment Epithelial Cells In Vitro: Contribution to Cell Viability and Proliferation, and Down-Regulation by Vascular Endothelial Growth Factor
Authors: MC Beer, H Kuhrt, L Kohen, P Wiedemann, A Bringmann, M Hollborn
Biomolecules, 2022-06-18;12(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Genetic Characterization of Rat Hepatic Stellate Cell Line HSC-T6 for In Vitro Cell Line Authentication
Authors: I Nanda, C Steinlein, T Haaf, EM Buhl, DG Grimm, SL Friedman, SK Meurer, SK Schröder, R Weiskirche
Cells, 2022-05-29;11(11):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Modified lipid metabolism and cytosolic phospholipase A2 activation in mesangial cells under pro-inflammatory conditions
Authors: R Boi, K Ebefors, M Henricsson, J Borén, J Nyström
Scientific Reports, 2022-05-05;12(1):7322.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
SM22alpha-lineage niche cells regulate intramembranous bone regeneration via PDGFRbeta-triggered hydrogen sulfide production
Authors: X Zhou, J Liu, Y Zheng, Z Zhang, Y Wu, W Yang, J Liu, Y Huang, Y Yi, Z Zhao, H Xiao, X Mo, J Wang
Cell Reports, 2022-05-03;39(5):110750.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Physical and functional interactome atlas of human receptor tyrosine kinases.
Authors: Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M
EMBO Rep, 2022-04-05;23(6):e54041.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay -
Overexpression of CEACAM6 activates Src-FAK signaling and inhibits anoikis, through homophilic interactions in lung adenocarcinomas.
Authors: Kim E, Cha Y, Jeong S, Chang Y
Transl Oncol, 2022-03-28;20(0):101402.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Arsenic trioxide inhibits the functions of lung fibroblasts derived from patients with idiopathic pulmonary fibrosis
Authors: A Joannes, C Morzadec, M Duclos, FL Gutierrez, DC Chiforeanu, C Le Naoures, B De Latour, S Rouzé, L Wollin, S Jouneau, L Vernhet
Toxicology and Applied Pharmacology, 2022-03-09;441(0):115972.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
MiR-34a-3p suppresses pulmonary vascular proliferation in acute pulmonary embolism rat by targeting DUSP1
Authors: Y Li, J Shao, J Song, S Yu, J Wang, K Sun
Bioscience Reports, 2022-01-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
International Journal of Molecular Sciences, 2022-01-14;23(2):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
Authors: M Culenova, A Nicodemou, ZV Novakova, M Debreova, V Smolinská, S Bernatova, D Ivanisova, O Novotna, J Vasicek, I Varga, S Ziaran, L Danisovic
International Journal of Molecular Sciences, 2021-11-19;22(22):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Gold Nanoparticles Affect Pericyte Biology and Capillary Tube Formation
Authors: S Looprasert, A Sereemaspu, N Kitkumthor, K Sooklert, T Sarachana, D Jindatip
Pharmaceutics, 2021-05-17;13(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Ketogenic Diet Enhances the Cholesterol Accumulation in Liver and Augments the Severity of CCl4 and TAA-Induced Liver Fibrosis in Mice
Authors: YJ Liao, YH Wang, CY Wu, FY Hsu, CY Chien, YC Lee
International Journal of Molecular Sciences, 2021-03-13;22(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Long noncoding RNA ERLR mediates epithelial-mesenchymal transition of retinal pigment epithelial cells and promotes experimental proliferative vitreoretinopathy
Authors: S Yang, H Li, H Yao, Y Zhang, H Bao, L Wu, C Zhang, M Li, L Feng, J Zhang, Z Zheng, G Xu, F Wang
Cell Death and Differentiation, 2021-03-04;0(0):.
Species: Rabbit
Sample Types: In Vivo
Applications: Bioassay -
DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously
Authors: R Beckmann, K Jensen, S Fenn, J Speck, K Krause, A Meier, M Röth, S Fauser, R Kimbung, DT Logan, M Steegmaier, H Kettenberg
Nature Communications, 2021-01-29;12(1):708.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Standard -
Control of mesenchymal cell fate via application of FGF-8b in vitro
Authors: T Otsuka, PY Mengsteab, CT Laurencin
Stem Cell Research, 2021-01-07;51(0):102155.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Zinc salicylate reduces airway smooth muscle cells remodelling by blocking mTOR and activating p21(Waf1/Cip1)
Authors: L Fang, M Roth, C Teck S'ng, M Tamm, B Han, BX Hoang
The Journal of nutritional biochemistry, 2020-12-14;0(0):108563.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pluripotent epigenetic regulator OBP-801 maintains filtering blebs in glaucoma filtration surgery model
Authors: Y Yamamoto, A Mukai, T Ikushima, Y Urata, S Kinoshita, J Hamuro, M Ueno, C Sotozono
Scientific Reports, 2020-12-01;10(1):20936.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Proto-oncogene Src links lipogenesis via lipin-1 to breast cancer malignancy
Authors: L Song, Z Liu, HH Hu, Y Yang, TY Li, ZZ Lin, J Ye, J Chen, X Huang, DT Liu, J Zhou, Y Shi, H Zhao, C Xie, L Chen, E Song, SY Lin, SC Lin
Nat Commun, 2020-11-17;11(1):5842.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease
Authors: K Song, Y Qing, Q Guo, EK Peden, C Chen, WE Mitch, L Truong, J Cheng
JCI Insight, 2020-11-05;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner
Authors: YY Liang, XB Deng, XT Lin, LL Jiang, XT Huang, ZW Mo, YW Yuan, MT Teh
Cell Death Dis, 2020-10-14;11(10):855.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differential regulation of Lipocalin 2 (LCN2) in doxorubicin-resistant 4T1 triple negative breast cancer cells
Authors: SK Meurer, O Tezcan, T Lammers, R Weiskirche
Cell. Signal., 2020-08-03;74(0):109731.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TGFb acts through PDGFRb to activate mTORC1 via the Akt/PRAS40 axis and causes glomerular mesangial cell hypertrophy and matrix protein expression
Authors: S Maity, F Das, BS Kasinath, N Ghosh-Chou, G Ghosh Chou
J. Biol. Chem., 2020-07-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Isolation and characterization of neural crest-like progenitor cells in human umbilical cord blood
Authors: Z Al-Bakri, M Ishige-Wad, N Fukuda, C Yoshida-No, N Nagoshi, H Okano, H Mugishima, T Matsumoto
Regenerative Therapy, 2020-07-18;15(0):53-63.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Byakangelicin protects against carbon tetrachloride-induced liver injury and fibrosis in mice
Authors: X Li, S Shao, H Li, Z Bi, S Zhang, Y Wei, J Bai, R Zhang, X Ma, B Ma, L Zhang, C Xie, W Ning, H Zhou, C Yang
J. Cell. Mol. Med., 2020-07-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Dedifferentiation of smooth muscle cells in intracranial aneurysms and its potential contribution to the pathogenesis
Authors: M Oka, S Shimo, N Ohno, H Imai, Y Abekura, H Koseki, H Miyata, K Shimizu, M Kushamae, I Ono, K Nozaki, A Kawashima, T Kawamata, T Aoki
Sci Rep, 2020-05-20;10(1):8330.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Notch signalling drives synovial fibroblast identity and arthritis pathology
Authors: K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner
Nature, 2020-04-22;582(7811):259-264.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Standing wave-assisted acoustic droplet vaporization for single and dual payload release in acoustically-responsive scaffolds
Authors: M Aliabouzar, A Jivani, X Lu, OD Kripfgans, JB Fowlkes, ML Fabiilli
Ultrason Sonochem, 2020-03-26;66(0):105109.
Species: N/A
Sample Types: Complex Sample Type
Applications: Bioassay -
Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells
Authors: W Chen, J Lin, B Li, S Cao, H Li, J Zhao, K Liu, Y Li, Y Li, S Sun
J. Cell. Mol. Med., 2020-03-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Vascular Smooth Muscle Cell-Derived Exosomal MicroRNAs Regulate Endothelial Cell Migration Under PDGF Stimulation
Authors: J Heo, HC Yang, WJ Rhee, H Kang
Cells, 2020-03-06;9(3):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
PERK Inhibition Mitigates Restenosis and�Thrombosis: A Potential Low-Thrombogenic Antirestenotic Paradigm
Authors: B Wang, M Zhang, G Urabe, Y Huang, G Chen, D Wheeler, DJ Dornbos, A Huttinger, SM Nimjee, S Gong, LW Guo, KC Kent
JACC Basic Transl Sci, 2020-02-19;5(3):245-263.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Adult Human Dermal Progenitor Cell Transplantation Modulates the Functional Outcome of Split-Thickness Skin Xenografts
Authors: NA Agabalyan, HD Sparks, S Tarraf, NL Rosin, K Anker, G Yoon, LN Burnett, D Nickerson, ES Di Martino, VA Gabriel, J Biernaskie
Stem Cell Reports, 2019-11-14;13(6):1068-1082.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
miR-29a Regulates the Proliferation and Migration of Human Arterial Smooth Muscle Cells in Arteriosclerosis Obliterans of the Lower Extremities
Authors: K Wang, J Yu, B Wang, H Wang, Z Shi, G Li
Kidney Blood Press. Res., 2019-10-15;44(5):1219-1232.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-γ enhances the antifibrotic effects of pirfenidone by attenuating IPF lung fibroblast activation and differentiation
Authors: TN Vu, X Chen, HD Foda, GC Smaldone, NA Hasaneen
Respir. Res., 2019-09-11;20(1):206.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation
Authors: L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn
Mol. Vis., 2019-06-30;25(0):329-344.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NLRC5 inhibits neointima formation following vascular injury and directly interacts with PPARgamma
Authors: P Luan, W Jian, X Xu, W Kou, Q Yu, H Hu, D Li, W Wang, MW Feinberg, J Zhuang, Y Xu, W Peng
Nat Commun, 2019-06-28;10(1):2882.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
J. Dermatol. Sci., 2019-05-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Proximity hybridization-induced on particle DNA walker for ultrasensitive protein detection
Authors: H Gan, J Wu, H Ju
Anal. Chim. Acta, 2019-05-08;1074(0):142-149.
Species: N/A
Sample Types: DNA
Applications: Bioassay -
Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels
Authors: T Emi, K Michaud, E Orton, G Santilli, C Linh, M O'Connell, F Issa, S Kennedy
Molecules, 2019-03-16;24(6):.
Applications: Bioassay -
Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
Cell Rep, 2019-03-05;26(10):2566-2579.e10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells
Authors: MFM Gerli, LA Moyle, S Benedetti, G Ferrari, E Ucuncu, M Ragazzi, C Constantin, I Louca, H Sakai, P Ala, P De Coppi, S Tajbakhsh, G Cossu, FS Tedesco
Stem Cell Reports, 2019-02-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Minocycline inhibits PDGF-BB-induced human aortic smooth muscle cell proliferation and migration by reversing miR-221- and -222-mediated RECK suppression
Authors: Y Higashi, S Mummidi, S Sukhanov, T Yoshida, M Noda, P Delafontai, B Chandrasek
Cell. Signal., 2019-02-01;57(0):20-Oct.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Possible contribution of PDGF-BB-induced autophagy in dermatofibrosarcoma protuberans: Autophagy marker Atg5 could be a differential marker between dermatofibrosarcoma protuberans and dermatofibroma
Authors: SI Motegi, C Fujiwara, A Sekiguchi, S Yamazaki, Y Yokoyama, M Yasuda, O Ishikawa
J. Dermatol. Sci., 2019-01-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tyrosyl phosphorylation of KRAS stalls GTPase cycle via alteration of switch I and II conformation
Authors: Y Kano, T Gebregiwor, CB Marshall, N Radulovich, BPK Poon, J St-Germain, JD Cook, I Valencia-S, BMM Grant, SG Herrera, J Miao, B Raught, MS Irwin, JE Lee, JJ Yeh, ZY Zhang, MS Tsao, M Ikura, M Ohh
Nat Commun, 2019-01-15;10(1):224.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
MicroRNA-320 targeting neuropilin 1 inhibits proliferation and migration of vascular smooth muscle cells and neointimal formation
Authors: H Li, J Zhao, B Liu, J Luo, Z Li, X Qin, Y Wei
Int J Med Sci, 2019-01-01;16(1):106-114.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity
Authors: M Zhang, G Urabe, C Little, B Wang, AM Kent, Y Huang, KC Kent, LW Guo
JACC Basic Transl Sci, 2018-12-31;3(6):782-795.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Barrier properties and transcriptome expression in human iPSC-derived models of the blood-brain barrier
Authors: L Delsing, P Dönnes, J Sánchez, M Clausen, D Voulgaris, A Falk, A Herland, G Brolén, H Zetterberg, R Hicks, J Synnergren
Stem Cells, 2018-11-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mechanism of anti-remodelling action of treprostinil in human pulmonary arterial smooth muscle cells
Authors: C Lambers, C Kornauth, F Oberndorfe, PM Boehm, M Tamm, W Klepetko, M Roth
PLoS ONE, 2018-11-01;13(11):e0205195.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy
Authors: A Dubrac, SE Künzel, SH Künzel, J Li, RR Chandran, K Martin, DM Greif, RH Adams, A Eichmann
Nat Commun, 2018-08-27;9(1):3463.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Telomere shortening is a hallmark of genetic cardiomyopathies
Authors: ACY Chang, ACH Chang, A Kirillova, K Sasagawa, W Su, G Weber, J Lin, V Termglinch, I Karakikes, T Seeger, AM Dainis, JT Hinson, J Seidman, CE Seidman, JW Day, E Ashley, JC Wu, HM Blau
Proc. Natl. Acad. Sci. U.S.A., 2018-08-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells
Authors: R Sambathkum, R Akkerman, S Dastidar, P Roelandt, M Kumar, M Bajaj, AR Mestre Ros, N Helsen, V Vanslembro, E Kalo, S Khurana, J Laureys, C Gysemans, MM Faas, P de Vos, CM Verfaillie
PLoS ONE, 2018-05-11;13(5):e0197046.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
FOXM1 is a critical driver of lung fibroblast activation and fibrogenesis
Authors: LR Penke, JM Speth, VL Dommeti, ES White, IL Bergin, M Peters-Gol
J. Clin. Invest., 2018-05-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Protective Effects of Fucoidan on Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells and Progression of Proliferative Vitreoretinopathy
Authors: Y Zhang, D Zhao, S Yang, H Yao, M Li, C Zhao, J Zhang, GT Xu, H Li, F Wang
Cell. Physiol. Biochem., 2018-04-23;46(4):1704-1715.
Species: Rabbit
Sample Types: In Vivo
Applications: In Vivo -
Methotrexate inhibits effects of platelet-derived growth factor and interleukin-1? on rheumatoid arthritis fibroblast-like synoviocytes
Authors: B Bergström, H Carlsten, AH Ekwall
Arthritis Res. Ther., 2018-03-20;20(1):49.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain
Authors: JM Dave, T Mirabella, SD Weatherbee, DM Greif
Dev. Cell, 2018-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The chondroitin sulfate moiety mediates thrombomodulin-enhanced adhesion and migration of vascular smooth muscle cells
Authors: VC Pai, IC Lo, YW Huang, IC Tsai, HP Cheng, GY Shi, HL Wu, MJ Jiang
J. Biomed. Sci., 2018-02-13;25(1):14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Diverse functions of clusterin promote and protect against the development of pulmonary fibrosis
Authors: L Peix, IC Evans, DR Pearce, JK Simpson, TM Maher, RJ McAnulty
Sci Rep, 2018-01-30;8(1):1906.
Species: Human
Sample Types: Whole Cells
-
Postnatal Tendon Growth and Remodeling Requires Platelet-Derived Growth Factor Receptor Signaling
Authors: KB Sugg, JF Markworth, NP Disser, AM Rizzi, JR Talarek, DC Sarver, SV Brooks, CL Mendias
Am. J. Physiol., Cell Physiol., 2017-12-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
Sci Rep, 2017-11-27;7(1):16439.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Combination therapy using molecular-targeted drugs modulates tumor microenvironment and impairs tumor growth in renal cell carcinoma
Authors: H Kitano, Y Kitadai, J Teishima, R Yuge, S Shinmei, K Goto, S Inoue, T Hayashi, K Sentani, W Yasui, A Matsubara
Cancer Med, 2017-08-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the synthetic phenotype
Authors: TM Wang, KC Chen, PY Hsu, HF Lin, YS Wang, CY Chen, YC Liao, SH Juo
J. Cell. Mol. Med., 2017-07-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H2O2 exposure
Authors: M Dagnell, PE Pace, Q Cheng, J Frijhoff, A Östman, ESJ Arnér, MB Hampton, CC Winterbour
J. Biol. Chem., 2017-07-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Platelet-Derived Growth Factor BB Influences Muscle Regeneration in Duchenne Muscle Dystrophy
Authors: P Piñol-Jura, E Gallardo, N de Luna, X Suárez-Cal, C Sánchez-Ri, E Fernández-, C Gomis, I Illa, J Díaz-Maner
Am. J. Pathol., 2017-06-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Src-homology protein tyrosine phosphatase-1 agonist, SC-43, reduces liver fibrosis
Authors: TH Su, CW Shiau, P Jao, NJ Yang, WT Tai, CJ Liu, TC Tseng, HC Yang, CH Liu, KW Huang, TC Hu, YJ Huang, YM Wu, LJ Chen, PJ Chen, DS Chen, KF Chen, JH Kao
Sci Rep, 2017-05-11;7(1):1728.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
BMC Cell Biol., 2017-04-21;18(1):21.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
PLoS Pathog., 2017-04-12;13(4):e1006281.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Folic acid inhibits dedifferentiation of PDGF-BB-induced vascular smooth muscle cells by suppressing mTOR/P70S6K signaling
Authors: S Pan, H Lin, H Luo, F Gao, L Meng, C Zhou, C Jiang, Y Guo, Z Ji, J Chi, H Guo
Am J Transl Res, 2017-03-15;9(3):1307-1316.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Urethral reconstruction with autologous urine-derived stem cells seeded in three-dimensional porous small intestinal submucosa in a rabbit model
Authors: Y Liu, W Ma, B Liu, Y Wang, J Chu, G Xiong, L Shen, C Long, T Lin, D He, D Butnaru, L Alexey, Y Zhang, D Zhang, G Wei
Stem Cell Res Ther, 2017-03-09;8(1):63.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Isoquercitrin protects against pulmonary hypertension via inhibiting PASMCs proliferation
Authors: Haipeng Guo
Clin. Exp. Pharmacol. Physiol., 2017-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling
Authors: D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó
Kidney Blood Press. Res, 2017-02-28;42(1):16-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
c-Jun-N-terminal phosphorylation regulates DNMT1 expression and genome wide methylation in gliomas
Authors: DH Heiland, R Ferrarese, R Claus, F Dai, AP Masilamani, E Kling, A Weyerbrock, T Kling, S Nelander, MS Carro
Oncotarget, 2017-01-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Zedoarondiol Inhibits Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells Proliferation via Regulating AMP-Activated Protein Kinase Signaling Pathway
Authors: Huimin Mao
Cell. Physiol. Biochem, 2016-12-21;40(6):1506-1520.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Biological characteristics of embryonic epicardial cells in vitro correlate with embryonic day
Authors: Xiaoming Wei
Acta Biochim. Biophys. Sin. (Shanghai), 2016-12-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
miR-184 exhibits angiostatic properties via regulation of Akt and VEGF signaling pathways
Authors: Robert M Lavker
FASEB J., 2016-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-? blocks signalling through PDGFR? in human brain pericytes
J Neuroinflammation, 2016-09-21;13(1):249.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pericyte-fibroblast transition promotes tumor growth and metastasis
Proc Natl Acad Sci USA, 2016-09-07;113(38):E5618-E5627.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchymal Stem Cells Reshape and Provoke Proliferation of Articular Chondrocytes by Paracrine Secretion
Sci Rep, 2016-09-06;6(0):32705.
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchymal stem cells from cortical bone demonstrate increased clonal incidence, potency, and developmental capacity compared to their bone marrow-derived counterparts
J Tissue Eng, 2016-08-16;7(0):2041731416661.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Integrative functional genomics identifies regulatory mechanisms at coronary artery disease loci
Nat Commun, 2016-07-08;7(0):12092.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Platelet-Derived Growth Factor in the Ovarian Follicle Attracts the Stromal Cells of the Fallopian Tube Fimbriae
PLoS ONE, 2016-07-05;11(7):e0158266.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
Authors: Bogershausen N, Tsai I, Pohl E, Kiper P, Beleggia F, Percin E, Keupp K, Matchan A, Milz E, Alanay Y, Kayserili H, Liu Y, Banka S, Kranz A, Zenker M, Wieczorek D, Elcioglu N, Prontera P, Lyonnet S, Meitinger T, Stewart A, Donnai D, Strom T, Boduroglu K, Yigit G, Li Y, Katsanis N, Wollnik B
J Clin Invest, 2015-08-17;125(9):3585-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Fanconi Anemia Mesenchymal Stromal Cells-Derived Glycerophospholipids Skew Hematopoietic Stem Cell Differentiation Through Toll-Like Receptor Signaling.
Authors: Amarachintha S, Sertorio M, Wilson A, Li X, Pang Q
Stem Cells, 2015-07-24;33(11):3382-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Hyperplastic Growth of Pulmonary Artery Smooth Muscle Cells from Subjects with Pulmonary Arterial Hypertension Is Activated through JNK and p38 MAPK.
Authors: Wilson J, Yu J, Taylor L, Polgar P
2015-04-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pyrroloquinoline-quinone suppresses liver fibrogenesis in mice.
Authors: Jia D, Duan F, Peng P, Sun L, Ruan Y, Gu J
PLoS ONE, 2015-03-30;10(3):e0121939.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Thrombomodulin promotes corneal epithelial wound healing.
Authors: Huang, Yi-Hsun, I, Ching-Ch, Kuo, Cheng-Hs, Hsu, Yun-Yan, Lee, Fang-Tzu, Shi, Guey-Yue, Tseng, Sung-Hue, Wu, Hua-Lin
PLoS ONE, 2015-03-27;10(3):e0122491.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Hsp90alpha and Hsp90beta together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.
Authors: Jayaprakash P, Dong H, Zou M, Bhatia A, O'Brien K, Chen M, Woodley D, Li W
J Cell Sci, 2015-03-03;128(8):1475-80.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The matricellular protein Cyr61 is a key mediator of platelet-derived growth factor-induced cell migration.
Authors: Zhang F, Hao F, An D, Zeng L, Wang Y, Xu X, Cui M
J Biol Chem, 2015-01-26;290(13):8232-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Anti-fibrotic effects of nintedanib in lung fibroblasts derived from patients with idiopathic pulmonary fibrosis.
Authors: Hostettler K, Zhong J, Papakonstantinou E, Karakiulakis G, Tamm M, Seidel P, Sun Q, Mandal J, Lardinois D, Lambers C, Roth M
Respir Res, 2014-12-12;15(1):157.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human corneal stromal stem cells exhibit survival capacity following isolation from stored organ-culture corneas.
Authors: Kureshi A, Funderburgh J, Daniels J
Invest Ophthalmol Vis Sci, 2014-10-21;55(11):7583-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders.
Authors: Niu F, Yao H, Zhang W, Sutliff R, Buch S
J Neurosci, 2014-08-27;34(35):11812-25.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Lutein inhibits the migration of retinal pigment epithelial cells via cytosolic and mitochondrial Akt pathways (lutein inhibits RPE cells migration).
Authors: Su C, Chan C, Chen H, Wu C, Hsiao C, Lee P, Lin V, Hung C
Int J Mol Sci, 2014-08-08;15(8):13755-67.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rho GTPases RhoA and Rac1 mediate effects of dietary folate on metastatic potential of A549 cancer cells through the control of cofilin phosphorylation.
Authors: Oleinik N, Helke K, Kistner-Griffin E, Krupenko N, Krupenko S
J Biol Chem, 2014-08-01;289(38):26383-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells.
Authors: Yang, Wei, Ramachandran, Aruna, You, Sungyong, Jeong, HyoBin, Morley, Samantha, Mulone, Michelle, Logvinenko, Tanya, Kim, Jayoung, Hwang, Daehee, Freeman, Michael, Adam, Rosalyn
Cell Commun Signal, 2014-08-01;12(1):44.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cellular stress amplifies TLR3/4-induced CXCL1/2 gene transcription in mononuclear phagocytes via RIPK1.
Authors: Zhao C, Pavicic P, Datta S, Sun D, Novotny M, Hamilton T
J Immunol, 2014-06-11;193(2):879-88.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor.
Authors: Tsai T, Shih S, Ho T, Ma H, Liu M, Chen S, Tsao Y
PLoS ONE, 2014-04-24;9(4):e95443.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
PLoS ONE, 2014-04-08;9(4):e94114.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Coronary heart disease-associated variation in TCF21 disrupts a miR-224 binding site and miRNA-mediated regulation.
Authors: Miller C, Haas U, Diaz R, Leeper N, Kundu R, Patlolla B, Assimes T, Kaiser F, Perisic L, Hedin U, Maegdefessel L, Schunkert H, Erdmann J, Quertermous T, Sczakiel G
PLoS Genet, 2014-03-27;10(3):e1004263.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis.
Authors: Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J
PLoS ONE, 2014-03-25;9(3):e92925.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Imatinib mesylate exerts anti-proliferative effects on osteosarcoma cells and inhibits the tumour growth in immunocompetent murine models.
Authors: Gobin B, Moriceau G, Ory B, Charrier C, Brion R, Blanchard F, Redini F, Heymann D
PLoS ONE, 2014-03-05;9(3):e90795.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Bioassay -
Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis.
Authors: Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B
J Pharmacol Exp Ther, 2014-02-20;349(2):209-20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
Authors: Lo H, Lai T, Li C, Wu W
Acta Pharmacol Sin, 2014-02-03;35(3):339-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma.
Authors: Zhang, Y, Kim, J, Mueller, A C, Dey, B, Yang, Y, Lee, D-h, Hachmann, J, Finderle, S, Park, D M, Christensen, J, Schiff, D, Purow, B, Dutta, A, Abounader, R
Cell Death Differ, 2014-01-17;21(5):720-34.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer.
Authors: Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L
Mol Ther, 2014-01-02;22(4):828-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling.
Authors: Bastiaans J, van Meurs J, van Holten-Neelen C, Nagtzaam N, van Hagen P, Chambers R, Hooijkaas H, Dik W
Invest Ophthalmol Vis Sci, 2013-12-19;54(13):8306-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Src-mediated caveolin-1 phosphorylation affects the targeting of active Src to specific membrane sites.
Authors: Gottlieb-Abraham, Efrat, Shvartsman, Dmitry E, Donaldson, John C, Ehrlich, Marcelo, Gutman, Orit, Martin, G Steven, Henis, Yoav I
Mol Biol Cell, 2013-10-16;24(24):3881-95.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Bioassay -
Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration.
Authors: Gadepalli R, Kotla S, Heckle M, Verma S, Singh N, Rao G
J Biol Chem, 2013-09-11;288(43):30815-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Magnolol inhibits migration of vascular smooth muscle cells via cytoskeletal remodeling pathway to attenuate neointima formation.
Authors: Karki R, Kim S, Kim D
Exp Cell Res, 2013-07-29;319(20):3238-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cytotoxic effects of curcumin in human retinal pigment epithelial cells.
Authors: Hollborn M, Chen R, Wiedemann P, Reichenbach A, Bringmann A, Kohen L
PLoS ONE, 2013-03-26;8(3):e59603.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rapamycin inhibits smooth muscle cell proliferation and obstructive arteriopathy attributable to elastin deficiency.
Authors: Li, Wei, Li, Qingle, Qin, Lingfeng, Ali, Rahmat, Qyang, Yibing, Tassabehji, May, Pober, Barbara, Sessa, William, Giordano, Frank J, Tellides, George
Arterioscler Thromb Vasc Biol, 2013-03-14;33(5):1028-35.
Species: Human
Sample Types: Serum
Applications: Bioassay -
Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
Exp Cell Res, 2013-02-28;319(8):1136-45.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inactivation of nuclear factor-Y inhibits vascular smooth muscle cell proliferation and neointima formation.
Authors: Silvestre-Roig C, Fernandez P, Esteban V, Pello O, Indolfi C, Rodriguez C, Rodriguez-Calvo R, Lopez-Maderuelo M, Bauriedel G, Hutter R, Fuster V, Ibanez B, Redondo J, Martinez-Gonzalez J, Andres V
Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):1036-45.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Bioassay -
ROS-mediated downregulation of MYPT1 in smooth muscle cells: a potential mechanism for the aberrant contractility in atherosclerosis.
Authors: Cheng J, Cheng H, Tsai I, Jiang M
Lab Invest, 2013-02-18;93(4):422-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem Cells, 2012-07-01;30(7):1455-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
J. Biol. Chem., 2011-06-13;286(32):28097-110.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differentiation potential of human postnatal mesenchymal stem cells, mesoangioblasts, and multipotent adult progenitor cells reflected in their transcriptome and partially influenced by the culture conditions.
Authors: Roobrouck VD, Clavel C, Jacobs SA, Ulloa-Montoya F, Crippa S, Sohni A, Roberts SJ, Luyten FP, Van Gool SW, Sampaolesi M, Delforge M, Luttun A, Verfaillie CM
Stem Cells, 2011-05-01;29(5):871-82.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Neuropilin-1 signaling through p130Cas tyrosine phosphorylation is essential for growth factor-dependent migration of glioma and endothelial cells.
Authors: Evans IM, Yamaji M, Britton G, Pellet-Many C, Lockie C, Zachary IC, Frankel P
Mol. Cell. Biol., 2011-01-18;31(6):1174-85.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
PLoS ONE, 2010-08-11;5(8):e12101.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
FASEB J., 2010-05-19;24(10):3782-95.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymic stromal lymphopoietin receptor-mediated IL-6 and CC/CXC chemokines expression in human airway smooth muscle cells: role of MAPKs (ERK1/2, p38, and JNK) and STAT3 pathways.
Authors: Shan L, Redhu NS, Saleh A
J. Immunol., 2010-05-14;184(12):7134-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
Biochem. J., 2010-03-15;427(1):29-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
Oncogene, 2010-03-08;29(20):2938-49.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
KSHV-induced notch components render endothelial and mural cell characteristics and cell survival.
Authors: Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS
Blood, 2009-11-24;115(4):887-95.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Transactivation of RON receptor tyrosine kinase by interaction with PDGF receptor beta during steady-state growth of human mesangial cells.
Authors: Kobayashi T, Furukawa Y, Kikuchi J, Ito C, Miyata Y, Muto S, Tanaka A, Kusano E
Kidney Int., 2009-02-25;75(11):1173-83.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Imatinib mesylate and AMN107 inhibit PDGF-signaling in orbital fibroblasts: a potential treatment for Graves' ophthalmopathy.
Authors: van Steensel L, Paridaens D, Schrijver B, Dingjan GM, van Daele PL, van Hagen PM, van den Bosch WA, Drexhage HA, Hooijkaas H, Dik WA
Invest. Ophthalmol. Vis. Sci., 2009-02-21;50(7):3091-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Small molecule inhibitors of Hsp90 potently affect inflammatory disease pathways and exhibit activity in models of rheumatoid arthritis.
Authors: Rice JW, Veal JM, Fadden RP, Barabasz AF, Partridge JM, Barta TE, Dubois LG, Huang KH, Mabbett SR, Silinski MA, Steed PM, Hall SE
Arthritis Rheum., 2008-12-01;58(12):3765-75.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ang II-stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice.
Authors: Jiang M, Bujo H, Ohwaki K, Unoki H, Yamazaki H, Kanaki T, Shibasaki M, Azuma K, Harigaya K, Schneider WJ, Saito Y
J. Clin. Invest., 2008-08-01;118(8):2733-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
BIBF 1120: triple angiokinase inhibitor with sustained receptor blockade and good antitumor efficacy.
Authors: Hilberg F, Roth GJ, Krssak M, Kautschitsch S, Sommergruber W, Tontsch-Grunt U, Garin-Chesa P, Bader G, Zoephel A, Quant J, Heckel A, Rettig WJ
Cancer Res., 2008-06-15;68(12):4774-82.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
Atherosclerosis, 2008-05-22;202(2):415-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Connective tissue growth factor as a mediator of intraocular fibrosis.
Authors: He S, Chen Y, Khankan R, Barron E, Burton R, Zhu D, Ryan SJ, Oliver N, Hinton DR
Invest. Ophthalmol. Vis. Sci., 2008-04-30;49(9):4078-88.
Species: Rabbit
Sample Types: In Vivo
Applications: In Vivo -
A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
Exp. Cell Res., 2008-04-25;314(11):2176-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
Authors: Tomanek RJ, Hansen HK, Christensen LP
Arterioscler. Thromb. Vasc. Biol., 2008-04-17;28(7):1237-43.
Species: Avian
Sample Types: Whole Tissue
Applications: Bioassay -
Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
Authors: Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC
Exp. Cell Res., 2008-03-05;314(8):1721-33.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Authors: Li R, Maminishkis A, Wang FE, Miller SS
Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells.
Authors: Schweizer PA, Krause U, Becker R, Seckinger A, Bauer A, Hardt C, Eckstein V, Ho AD, Koenen M, Katus HA, Zehelein J
Stem Cells, 2007-06-01;25(6):1546-51.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007-04-30;177(3):489-500.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells.
Authors: Tacke F, Gabele E, Bataille F, Schwabe RF, Hellerbrand C, Klebl F, Straub RH, Luedde T, Manns MP, Trautwein C, Brenner DA, Scholmerich J, Schnabl B
Dig. Dis. Sci., 2007-04-06;52(12):3404-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
Blood, 2007-02-08;109(10):4264-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
Authors: Hunter I, Nixon GF
J. Biol. Chem., 2006-09-18;281(45):34705-15.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Multipotent adult progenitor cells from swine bone marrow.
Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
Stem Cells, 2006-08-24;24(11):2355-66.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Pitavastatin attenuates the PDGF-induced LR11/uPA receptor-mediated migration of smooth muscle cells.
Authors: Jiang M, Bujo H, Zhu Y, Yamazaki H, Hirayama S, Kanaki T, Shibasaki M, Takahashi K, Schneider WJ, Saito Y
Biochem. Biophys. Res. Commun., 2006-08-10;348(4):1367-77.
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay -
Expression of the FUS-CHOP fusion protein in primary mesenchymal progenitor cells gives rise to a model of myxoid liposarcoma.
Authors: Riggi N, Cironi L, Provero P, Suva ML, Stehle JC, Baumer K, Guillou L, Stamenkovic I
Cancer Res., 2006-07-15;66(14):7016-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
Blood, 2005-12-01;107(5):1896-902.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
J. Cell. Physiol., 2005-04-01;203(1):35-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
Authors: Wong AP, Nili N, Strauss BH
Cardiovasc. Res., 2005-02-15;65(3):702-10.
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay -
Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.
Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
Arthritis Rheum., 2004-08-01;50(8):2521-30.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
Am. J. Respir. Cell Mol. Biol., 2004-01-12;30(6):816-22.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
Authors: Lacoste E, Martineau I, Gagnon G
J. Periodontol., 2003-10-01;74(10):1498-507.
Applications: ELISA (Standard) -
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
Am. J. Pathol., 2003-09-01;163(3):869-78.
Species: Rabbit
Sample Types: Whole Cells
Applications: Binding Assay, Bioassay -
Sef inhibits fibroblast growth factor signaling by inhibiting FGFR1 tyrosine phosphorylation and subsequent ERK activation.
Authors: Kovalenko D, Yang X, Nadeau RJ, Harkins LK, Friesel R
J. Biol. Chem., 2003-02-24;278(16):14087-91.
Species: Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Bioassay -
Dual roles of IL-4 in lung injury and fibrosis.
Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
Proteins containing BRCA1 C-terminal (BRCT) domains play crucial roles in response to and repair of DNA damage. Epithelial cell transforming factor ECT2 is a member of the BRCT protein family, but it, 2003-02-15;170(4):2083-92.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
Authors: Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Growth factors stimulate neointimal cells in vitro and increase the thickness of the neointima formed at the neck of porcine aneurysms treated by embolization.
Authors: Desfaits AC, 48971, Raymond J, Muizelaar JP
Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions, 2000-02-01;31(0):498-507.
Species: Canine, Porcine
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
Cancer Res., 1998-09-01;58(17):4008-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Can Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB) be reconstituted in a buffer containing BSA?
BSA preparations may contain minor impurities that can impact assay results, and assays involving Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB) can be influenced by these minor impurities. For this reason, we recommend reconstituting 220-BB to 100 ug/mL in 4 mM HCl. Including BSA in a buffer may be beneficial when diluting proteins to working concentrations less than 10 ug/mL. If BSA is included in an experiment involving 220-BB, we would recommend running a BSA-only control.
Reviews for Recombinant Human PDGF-BB Protein, CF
Average Rating: 4.5 (Based on 11 Reviews)
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Reason for Rating: This was a great control for my cell assay. What a reliable product.
Reason for Rating: Great for proof of concept, will see how it performs in a biological assay