Recombinant Human PDGF-BB, Biotinylated Protein

Catalog #: BT220
2 Citations Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
BT220-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
BT220-010

Recombinant Human PDGF-BB, Biotinylated Protein Bioactivity

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All PDGF-BB Proteins and Enzymes

Product Details

Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PDGF-BB, Biotinylated Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 1.5-6 ng/mL.

Source

E. coli-derived human PDGF-BB protein
Ser82-Thr190

Accession #

P01127.1

Structure / Form

Disulfide-linked homodimer, biotinylated protein via amines

Predicted Molecular Mass

12 kDa (unlabeled)

SDS-PAGE

12 kDa, reducing conditions

Product Datasheets

BT220 (with carrier)

Product Datasheet

BT220/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT220

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

BT220/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Both Biotinylated Recombinant Human PDGF-BB (Catalog # BT220) and unlabeled Recombinant Human PDGF-BB (Catalog # 220-BB) stimulate NR6R-3T3 mouse fibroblast cell proliferation. The ED₅₀ for this effect is 1.5-6 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Background: PDGF-BB

Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a 35 kDa, 241 amino acid (aa) prepro-precurser. It contains a signal peptide, an N-terminal prodomain, a mature region, and a C-terminal prodomain (1-4). The propercursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain. The resulting mature region is 16-17 kDa in size (or 29-32 kDa as a homodimer) (4). Mature human PDGF-B shares 89% aa sequence identity with mouse mature PDGF-B. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10).

References

Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392.
Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250.
Ostman, A. et al. (1992) J. Cell Biol. 118:509.
Siegfried, G. et al. (2005) Oncogene 24:6925.
Kreja, L. et al. (2010) J. Cell. Biochem. 109:347.
van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400.
Barrientos, S. et al. (2008) Wound Repair Regen. 16:585.
Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91.

Long Name

Platelet-derived Growth Factor BB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Alternate Names

c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

Related Research Areas

Citations for Recombinant Human PDGF-BB, Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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