Recombinant Human PDGF-AB Protein, CF Summary
Product Specifications
| Human PDGF-A (Ser87 - Thr211) Accession # P04085 |
| Human PDGF-B (Ser82 - Thr190) Accession # P01127 | ||
| N-terminus | C-terminus | |
Analysis
Product Datasheets
222-AB
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
222-AB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PDGF-AB
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
Citations for Recombinant Human PDGF-AB Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors
Authors: Madsen, SF;Sand, JMB;Juhl, P;Karsdal, M;Thudium, CS;Siebuhr, AS;Bay-Jensen, AC;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosis
Authors: MR Hadjichara, BT Roux, E Csomor, CA Feghali-Bo, LA Murray, DL Clarke, MA Lindsay
Sci Rep, 2019-04-15;9(1):6020.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
Sci Rep, 2017-11-27;7(1):16439.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
Sci Rep, 2017-03-02;7(0):43736.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis.
Authors: Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J
PLoS ONE, 2014-03-25;9(3):e92925.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
Authors: Ding W, Knox TR, Tschumper RC
Blood, 2010-07-06;116(16):2984-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
Biochem. J., 2010-03-15;427(1):29-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Authors: Li R, Maminishkis A, Wang FE, Miller SS
Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.
Authors: Gupta S, Ramjaun AR, Haiko P, Wang Y, Warne PH, Nicke B, Nye E, Stamp G, Alitalo K, Downward J
Cell, 2007-06-01;129(5):957-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
Authors: Rhodes KE, Raivich G, Fawcett JW
Neuroscience, 2006-04-21;140(1):87-100.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
J. Cell. Physiol., 2005-04-01;203(1):35-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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