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Recombinant Human P-Cadherin Fc Chimera Avi-tag Protein, CF

Catalog #: AVI11651 Datasheet
Catalog # Availability Size / Price Qty
AVI11651-050
Biotinylated Recombinant Human P‑Cadherin Fc Chimera Avi-tag Protein Binding Activity.
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Recombinant Human P-Cadherin Fc Chimera Avi-tag Protein, CF Summary

  • R&D Systems HEK293-derived Recombinant Human P-Cadherin Fc Chimera Avi-tag Protein (AVI11651)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human P-Cadherin Fc Chimera Avi-tag (Catalog # AVI11651) binds Recombinant Human CDCP1 Fc Chimera (Catalog # 10402-CU) with an ED50 of 0.500-7.00 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human P-Cadherin protein
Human PCAD
(Asp108-Gly654)
Accession # CAA45177.1		IEGRMDGGHuman IgG1
(Pro100-Lys330)		Avi-tag
N-terminusC-terminus
N-terminal Sequence
Analysis
Asp108
Structure / Form
Disulfide linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
88 kDa
SDS-PAGE
103-130 kDa, under reducing conditions.

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AVI11651

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11651

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Biotinylated Recombinant Human P-Cadherin Fc Chimera Avi-tag Protein (Catalog # AVI11651) binds Recombinant Human CDCP1 Fc Chimera (10402-CU) with an ED50 of 0.500-7.00 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human P‑Cadherin Fc Chimera Avi-tag Protein (Catalog # AVI11651) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 103-130 kDa.

Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology. Our Avi-tag Biotinylated human P-Cadherin Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

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