Recombinant Human Osteopontin (OPN) Protein
Recombinant Human Osteopontin (OPN) Protein Summary
Product Specifications
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.100-0.600 µg/mL.
Ile17-Asn300, with a C-terminal 6-His tag
Analysis
Product Datasheets
1433-OP (with carrier)
1433-OP/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1433-OP
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1433-OP/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
Recombinant Human Osteopontin/OPN (Catalog # 1433‑OP), when immobilized, supports the adhesion of the HEK293 human embryonic kidney cells.
1 μg/lane of Recombinant Human Osteopontin/OPN was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a bands at 60-65 kDa.
Background: Osteopontin/OPN
Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Human OPN is synthesized as a 317 amino acid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C-terminal CD44 binding site (1-4). Depending on
tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury (6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SVVYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).
- Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
- Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
- Young, M.F. et al. (1990) Genomics 7:491.
- Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
- Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
- Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
- Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
- Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
- Takafuji, V. et al. (2007) Oncogene 26:6361.
- Kiefer, F.W. et al. (2010) Diabetes 59:935.
Citations for Recombinant Human Osteopontin (OPN) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Single-cell RNA sequencing reveals the CRTAC1+ population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1+ macrophage
Authors: Tang, Y;Zhuo, D;Yu, Y;Pu, W;Ma, Y;Zhang, Y;Huang, Y;Zhang, Q;Tang, K;Meng, C;Yang, D;Bai, L;He, D;Jin, L;Zou, H;Xu, H;Zhu, Q;Wang, J;Chen, Y;Liu, J;
Aging cell
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The CEBPB+ glioblastoma subcluster specifically drives the formation of M2 tumor-associated macrophages to promote malignancy growth
Authors: Yang, Y;Jin, X;Xie, Y;Ning, C;Ai, Y;Wei, H;Xu, X;Ge, X;Yi, T;Huang, Q;Yang, X;Jiang, T;Wang, X;Piao, Y;Jin, X;
Theranostics
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An immunosuppressive vascular niche drives macrophage polarization and immunotherapy resistance in glioblastoma
Authors: Yang, F;Akhtar, MN;Zhang, D;El-Mayta, R;Shin, J;Dorsey, JF;Zhang, L;Xu, X;Guo, W;Bagley, SJ;Fuchs, SY;Koumenis, C;Lathia, JD;Mitchell, MJ;Gong, Y;Fan, Y;
Science advances
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Increased expression of OPN contributes to idiopathic pulmonary fibrosis and indicates a poor prognosis
Authors: Ji, J;Zheng, S;Liu, Y;Xie, T;Zhu, X;Nie, Y;Shen, Y;Han, X;
Journal of translational medicine
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A Dual Role of Osteopontin in Modifying B Cell Responses
Authors: Chunder, R;Schropp, V;Marzin, M;Amor, S;Kuerten, S;
Biomedicines
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin (OPN) alleviates the progression of osteoarthritis by promoting the anabolism of chondrocytes
Authors: Luo, W;Lin, Z;Yuan, Y;Wu, Z;Zhong, W;Liu, Q;
Genes & diseases
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis
Authors: A Umemoto, T Kuwada, K Murata, M Shiokawa, S Ota, Y Murotani, A Itamoto, K Nishitani, H Yoshitomi, T Fujii, A Onishi, H Onizawa, K Murakami, M Tanaka, H Ito, H Seno, A Morinobu, S Matsuda
Arthritis Research & Therapy, 2023-02-17;25(1):25.
Species: Human, N/A
Sample Types: Antibody, Protein, Whole Cells
Applications: Bioassay, ELISA Capture -
Differential Modulation of Human M1 and M2 Macrophage Activity by ICOS-Mediated ICOSL Triggering
Authors: CL Gigliotti, C Dianzani, I Stoppa, C Monge, S Sutti, D Sblattero, C Puricelli, R Rolla, U Dianzani, E Boggio
International Journal of Molecular Sciences, 2023-02-02;24(3):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin Exacerbates High-Fat Diet-Induced Metabolic Disorders in a Microbiome-Dependent Manner
Authors: J Chen, P Zeng, L Gong, X Zhang, Z Ling, K Bi, F Shi, K Wang, Q Zhang, J Jiang, Y Zhang, T Uede, EM El-Omar, H Diao
MBio, 2022-10-27;0(0):e0253122.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Paper-based aptasensor for colorimetric detection of osteopontin
Authors: AC Pereira, FTC Moreira, LR Rodrigues, MGF Sales
Analytica chimica acta, 2022-01-29;1198(0):339557.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
Smooth muscle-specific MMP17 (MT4-MMP) regulates the intestinal stem cell niche and regeneration after damage
Authors: M Martín-Alo, S Iqbal, PM Vornewald, HT Lindholm, MJ Damen, F Martínez, S Hoel, A Díez-Sánch, M Altelaar, P Katajisto, AG Arroyo, MJ Oudhoff
Nature Communications, 2021-11-18;12(1):6741.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8
Authors: S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström
The Journal of Biological Chemistry, 2021-10-21;0(0):101323.
Species: Human
Sample Types: Protein
Applications: Cleavage -
Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling
Authors: X Jiang, F Zhang, X Ji, F Dong, H Yu, M Xue, Y Qiu, F Yang, X Hu, Z Bao
Cytokine, 2021-02-27;142(0):155474.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The interaction between cancer associated fibroblasts and tumor associated macrophages via the osteopontin pathway in the tumor microenvironment of hepatocellular carcinoma
Authors: K Tokuda, Y Morine, K Miyazaki, S Yamada, Y Saito, M Nishi, T Tokunaga, T Ikemoto, S Imura, M Shimada
Oncotarget, 2021-02-16;12(4):333-343.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Absence of nuclear receptors LXRs impairs immune response to androgen deprivation and leads to prostate neoplasia
Authors: L Bousset, A Septier, J Bunay, A Voisin, R Guiton, C Damon-Soub, Y Renaud, A De Haze, V Sapin, A Fogli, A Rambur, C De Joussin, A Kocer, A Trousson, J Henry-Berg, M Höring, G Liebisch, S Matysik, JA Lobaccaro, L Morel, S Baron
PloS Biology, 2020-12-07;18(12):e3000948.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
Authors: T Haraguchi, Y Ayukawa, Y Shibata, T Takeshita, I Atsuta, Y Ogino, N Yasunami, Y Yamashita, K Koyano
J Clin Med, 2020-08-13;9(8):.
Species: Human, N/A
Sample Types: Protein, Substrate
Applications: Bioassay, ELISA Capture -
Co-culture of human fibroblasts, smooth muscle and endothelial cells promotes osteopontin induction in hypoxia
Authors: N Sadaghianl, J Contenti, M Dufies, J Parola, M Rouleau, S Lee, JF Peyron, L Fabbri, R Hassen-Kho, J Pouysségur, F Bost, E Jean-Bapti, A Dardik, NM Mazure
J. Cell. Mol. Med., 2020-02-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
NK cells induce hepatic ER stress to promote insulin resistance in obesity through osteopontin production
Authors: J Wu, D Wu, L Zhang, C Lin, J Liao, R Xie, Z Li, S Wu, A Liu, W Hu, Y Xi, S Bu, F Wang
J. Leukoc. Biol., 2019-12-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Development of a novel and rapid measurement system for growth differentiation factor-15, progranulin, and osteopontin in uterine sarcoma
Authors: M Maeno, T Mizutani, H Tsuyoshi, S Yamada, S Ishikane, S Kawabe, K Nishimura, M Yamada, K Miyamoto, Y Yoshida
Endocr. J., 2019-09-18;0(0):.
Species: Human
Sample Types: Serum
Applications: Standard -
A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
Authors: J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang
Clin Proteomics, 2019-03-05;16(0):10.
Species: Human
Sample Types: Serum
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Osteopontin mediates glioblastoma-associated macrophage infiltration and is a therapeutic target
Authors: J Wei, A Marisetty, B Schrand, K Gabrusiewi, Y Hashimoto, M Ott, Z Grami, LY Kong, X Ling, HG Caruso, S Zhou, YA Wang, GN Fuller, JT Huse, E Gilboa, N Kang, X Huang, R Verhaak, S Li, AB Heimberger
J. Clin. Invest., 2018-11-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion
Authors: JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu
J. Clin. Invest., 2018-11-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The osteopontin-CD44 axis in hepatic cancer stem cells regulates IFN signaling and HCV replication
Authors: T Shirasaki, M Honda, T Yamashita, K Nio, T Shimakami, R Shimizu, S Nakasyo, K Murai, N Shirasaki, H Okada, Y Sakai, T Sato, T Suzuki, K Yoshioka, S Kaneko
Sci Rep, 2018-09-03;8(1):13143.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay -
Osteopontin regulates HCV replication and assembly via interaction with HCV proteins and lipid droplets and by binding to receptors, αVβ3 and CD44
Authors: J Iqbal, M Sarkar-Dut, S McRae, A Ramachandr, B Kumar, G Waris
J. Virol., 2018-06-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
Immunol. Cell Biol., 2018-02-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Psoriatic cutaneous inflammation promotes human monocyte differentiation into active osteoclasts, facilitating bone damage
Authors: A Raimondo, S Lembo, RD Caprio, G Donnarumma, G Monfrecola, N Balato, F Ayala, A Balato
Eur. J. Immunol., 2017-05-02;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Cleaved Form of Osteopontin in Urine as a Clinical Marker of Lupus Nephritis
PLoS ONE, 2016-12-19;11(12):e0167141.
Species: Human
Sample Types: Urine
Applications: ELISA (Standard) -
Trametinib Drives T-cell-Dependent Control of KRAS-Mutated Tumors by Inhibiting Pathological Myelopoiesis
Cancer Res., 2016-11-01;76(21):6253-6265.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes
Authors: Guang-Hua Lei
Exp Ther Med, 2016-10-05;12(5):3488-3494.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Alcohol inhibits osteopontin-dependent transforming growth factor-beta1 expression in human mesenchymal stem cells.
Authors: Driver J, Weber C, Callaci J, Kothari A, Zapf M, Roper P, Borys D, Franzen C, Gupta G, Wai P, Zhang J, Denning M, Kuo P, Mi Z
J Biol Chem, 2015-02-24;290(16):9959-73.
Species: Human
Sample Types: Protein, Whole Cells
Applications: Bioassay -
Osteopontin mediates an MZF1-TGF-beta1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.
Authors: Weber C, Kothari A, Wai P, Li N, Driver J, Zapf M, Franzen C, Gupta G, Osipo C, Zlobin A, Syn W, Zhang J, Kuo P, Mi Z
Oncogene, 2014-12-22;34(37):4821-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
OPN induces FoxM1 expression and localization through ERK 1/2, AKT, and p38 signaling pathway in HEC-1A cells.
Authors: Xie, Yunpeng, Li, Yinghua, Kong, Ying
Int J Mol Sci, 2014-12-16;15(12):23345-58.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
Authors: Kato, Aki, Okura, Takafumi, Hamada, Chizuru, Miyoshi, Seigo, Katayama, Hitoshi, Higaki, Jitsuo, Ito, Ryoji
PLoS ONE, 2014-06-25;9(6):e100106.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin is induced by TGF-beta2 and regulates metabolic cell activity in cultured human optic nerve head astrocytes.
Authors: Neumann C, Garreis F, Paulsen F, Hammer C, Birke M, Scholz M
PLoS ONE, 2014-04-09;9(4):e92762.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Role of hepatitis C virus induced osteopontin in epithelial to mesenchymal transition, migration and invasion of hepatocytes.
Authors: Iqbal J, McRae S, Mai T, Banaudha K, Sarkar-Dutta M, Waris G
PLoS ONE, 2014-01-31;9(1):e87464.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dual regulation of osteopontin production by TLR stimulation in dendritic cells.
Authors: Salvi V, Scutera S, Rossi S, Zucca M, Alessandria M, Greco D, Bosisio D, Sozzani S, Musso T
J Leukoc Biol, 2013-04-22;94(1):147-58.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia.
Authors: Barros N, Hoac B, Neves R, Addison W, Assis D, Murshed M, Carmona A, McKee M
J Bone Miner Res, 2013-03-01;28(3):688-99.
Applications: Bioassay -
Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Human
Sample Types: Antibody, Recombinant Protein
Applications: Binding Assay, Surface Plasmon Resonance -
Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.
Authors: Arjomandi M, Frelinger J, Donde A
PLoS ONE, 2011-10-21;6(10):e25678.
Applications: Bioassay -
Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
Invest. Ophthalmol. Vis. Sci., 2011-09-09;52(10):7084-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
J. Hepatol., 2009-07-30;51(4):734-49.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
Authors: Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C
Am. J. Respir. Crit. Care Med., 2009-02-20;179(10):894-902.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
Authors: Shao J, Washington MK, Saxena R, Sheng H
Carcinogenesis, 2007-08-11;28(12):2476-83.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 4.5 (Based on 2 Reviews)
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Reason for Rating: Worked well with the corresponding Quantikine kit.