Recombinant Human MMP-9 Protein, CF Summary
Product Specifications
Ala20-Asp707 (Gln279Arg)
Analysis
Product Datasheets
911-MP
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
911-MP
Formulation | Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5 (TCNB)
- Recombinant Human MMP-9 (rhMMP-9) (Catalog # 911-MP)
- p-aminophenylmercuric acetate (APMA), (Sigma, Catalog # A-9563), prepare a 100 mM stock in DMSO
- Substrate: MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhMMP-9 to 100 µg/mL in Assay Buffer.
- Activate rhMMP-9 by adding APMA to a final concentration of 1 mM.
- Incubate at 37 °C for 24 hours.
- Dilute activated rhMMP-9 to 0.4 ng/µL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- Load 50 µL of the 0.4 ng/µL rhMMP-9 into a plate and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:- rhMMP-9: 0.020 µg
- Substrate: 10 µM
Scientific Data
Recombinant Human MMP-9 (Catalog # 911-MP) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. In addition to the human enzyme, the recombinant mouse MMP-9 is also available (Catalog # 909-MM).
Citations for Recombinant Human MMP-9 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
30
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
The Role of Macrophage Inhibitory Factor in TAA-Induced Liver Fibrosis in Mice: Modulatory Effects of Betaine
Authors: Radosavljevic, T;Vukicevic, D;Djureti?, J;Gopcevic, K;Labudovic Borovic, M;Stankovic, S;Samardzic, J;Radosavljevic, M;Vucevic, D;Jakovljevic, V;
Biomedicines
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Zymography -
Semaphorin 7A promotes endothelial permeability and inflammation via plexin C1 and integrin ?1 in Kawasaki disease
Authors: Huang, J;Zhao, C;Zhang, S;
BMC pediatrics
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Metformin Directly Binds to MMP-9 to Improve Plaque Stability
Authors: X Chen, S Wang, W Xu, M Zhao, Y Zhang, H Xiao
Journal of cardiovascular development and disease, 2023-01-30;10(2):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Dual Functionalized Liposomes for Selective Delivery of Poorly Soluble Drugs to Inflamed Brain Regions
Authors: S Giofrè, A Renda, S Sesana, B Formicola, B Vergani, BE Leone, V Denti, G Paglia, S Groppuso, V Romeo, L Muzio, A Balboni, A Menegon, A Antoniou, A Amenta, D Passarella, P Seneci, S Pellegrino, F Re
Pharmaceutics, 2022-11-07;14(11):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Verteporfin-mediated on/off photoswitching functions synergistically to treat choroidal vascular diseases
Authors: Y Ju, X Dai, Z Tang, Z Ming, N Ni, D Zhu, J Zhang, B Ma, J Wang, R Huang, S Zhao, Y Pang, P Gu
Bioactive materials, 2022-02-01;14(0):402-415.
Species: Human
Sample Types: Small Molecule
Applications: Bioassay -
Loss of mutual protection between human osteoclasts and chondrocytes in damaged joints initiates osteoclast-mediated cartilage degradation by MMPs
Authors: QC Larrouture, AP Cribbs, SR Rao, M Philpott, SJ Snelling, HJ Knowles
Scientific Reports, 2021-11-22;11(1):22708.
Species: Human
Sample Types: Recombinant Proteins
Applications: Zymography Control -
Predicting Proteolysis in Complex Proteomes Using Deep Learning
Authors: M Ozols, A Eckersley, CI Platt, C Stewart-Mc, SA Hibbert, J Revote, F Li, CEM Griffiths, REB Watson, J Song, M Bell, MJ Sherratt
International Journal of Molecular Sciences, 2021-03-17;22(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Matrix metalloproteinase-2, -7, and -9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases
Authors: M Määttä, HP Laurila, S Holopainen, K Aaltonen, L Lilja-Maul, S Viitanen, MM Rajamäki
Journal of veterinary internal medicine, 2020-12-04;0(0):.
Species: Canine
Sample Types:
Applications: Zymography Control -
Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model
Authors: T Bluhmki, S Bitzer, JA Gindele, E Schruf, T Kiechle, M Webster, J Schymeinsk, R Ries, F Gantner, D Bischoff, J Garnett, R Heilker
Sci Rep, 2020-08-03;10(1):13022.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: Bioassay, ELISA Capture -
Analysis of the inhibiting activity of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) on matrix metalloproteinases
Authors: SR Mendes, LD Amo-Maestr, L Marino-Pue, I Diego, T Goulas, FX Gomis-Rüth
Sci Rep, 2020-04-14;10(1):6317.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Wogonin Suppresses the Activity of Matrix Metalloproteinase-9 and Inhibits Migration and Invasion in Human Hepatocellular Carcinoma
Authors: M Hong, H Cheng, L Song, W Wang, Q Wang, D Xu, W Xing
Molecules, 2018-02-11;23(2):.
Applications: Bioassay -
Development of matrix metalloproteinase-targeted probes for lung inflammation detection with positron emission tomography
Authors: N Kondo, T Temma, K Aita, S Shimochi, K Koshino, M Senda, H Iida
Sci Rep, 2018-01-22;8(1):1347.
Applications: Bioassay -
Downregulation of monocytic differentiation via modulation of CD147 by 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors
Authors: MV Sasidhar, SK Chevooru, O Eickelberg, HP Hartung, O Neuhaus
PLoS ONE, 2017-12-18;12(12):e0189701.
Species: Human
Sample Types: Recombinant Protein
Applications: Zymography -
Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer
Authors: N Willumsen, CL Bager, DJ Leeming, AC Bay-Jensen, MA Karsdal
Neoplasia, 2017-03-07;19(4):271-278.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
Gelatinases A and B and Antioxidant Enzyme Activity in the Early Phase of Acute Myocardial Infarction
Authors: K Gopcevic, B Rovcanin, D Kekic, D Milasinovi, G Kocic, I Stojanovic
Folia Biol. (Praha), 2017-01-01;63(1):20-26.
Applications: Zymography -
IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
Authors: Matthew N Svalina
Sci Rep, 2016-06-03;6(0):27012.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pathogenic Upregulation of Glial Lipocalin-2 in the Parkinsonian Dopaminergic System
J Neurosci, 2016-05-18;36(20):5608-22.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
GDF15/MIC1 and MMP9 Cerebrospinal Fluid Levels in Parkinson's Disease and Lewy Body Dementia.
Authors: Walter Maetzler, Willy Deleersni, Valérie Hanssens, Alice Bernard, Kathrin Brockmann, Justus Marquetan, Isabel Wurster, Tim W Rattay, Lorenzo Roncoroni, Eva Schaeffer, Stefanie Lerche, Anja Apel, Christian Deuschle, Daniela Berg
PLoS ONE, 2016-03-03;0(0):1932-6203.
Species: Human
Sample Types: Beads
-
Plantamajoside, a potential anti-tumor herbal medicine inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of matrix metalloproteinase-9 and -2.
Authors: Pei S, Yang X, Wang H, Zhang H, Zhou B, Zhang D, Lin D
BMC Cancer, 2015-12-16;15(0):965.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
BMC Cancer, 2013-12-13;13(0):598.
-
Astrocytes directly influence tumor cell invasion and metastasis in vivo.
Authors: Wang, Ling, Cossette, Stephani, Rarick, Kevin R, Gershan, Jill, Dwinell, Michael, Harder, David R, Ramchandran, Ramani
PLoS ONE, 2013-12-04;8(12):e80933.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers.
Authors: Djokic J, Fagotto-Kaufmann C, Bartels R, Nelea V, Reinhardt D
J Biol Chem, 2013-06-19;288(31):22821-35.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
Simple pseudo-dipeptides with a P2' glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins.
Authors: Devel L, Beau F, Amoura M, Vera L, Cassar-Lajeunesse E, Garcia S, Czarny B, Stura E, Dive V
J Biol Chem, 2012-06-11;287(32):26647-56.
Applications: Enzyme Assay -
Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp).
Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y
J. Bone Miner. Res., 2011-01-01;26(0):220.
Species: Human
Sample Types:
Applications: Bioassay -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Applications: ELISA (Standard) -
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
Applications: Zymography -
Matrix metalloprotease-9 dysregulation in lower airway secretions of cystic fibrosis patients.
Authors: Gaggar A, Li Y, Weathington N, Winkler M, Kong M, Jackson P, Blalock JE, Clancy JP
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L96-L104.
Applications: Western Blot -
Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
Surgery, 2006-09-01;140(3):454-63.
Applications: Western Blot -
Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006-07-01;10(1):39-50.
Species: Human
Sample Types:
Applications: Enzyme Assay -
Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cells.
Authors: Levesque JP, Hendy J, Winkler IG, Takamatsu Y, Simmons PJ
Exp. Hematol., 2003-02-01;31(2):109-17.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay
FAQs
-
Can the enzyme be stored after activation, or do I need to use it immediately after activation?
We recommend only activating the amount of enzyme needed for your assay, and recommend activating the enzyme immediately prior to use. Any unactivated enzyme should be stored in aliquots at either the stock concentration at which the enzyme was supplied, or the reconstitution concentration, according to the product datasheet.
-
Does this enzyme have a tag?
No, this enzyme does not have a tag. Please refer to the Source section on the product-specific page or product datasheet for sequence information.
-
If I use this enzyme at a higher concentration, do I need to change the concentration of APMA to activate it?
We have only optimized activation conditions for one particular concentration of this MMP enzyme as part of our regular QC testing for enzymatic activity. Activating the enzyme at any different concentration would have to be optimized by the end user.
-
Does this MMP enzyme need to be activated to work?
Yes, this enzyme requires activation prior to use.
-
What is the activity of this enzyme in units/µg?
We supply this enzyme as a mass and calculate its activity relative to mass (pmol/min/µg). We have not calibrated this enzyme to an international standard unit, so we are unable to provide a conversion to units/µg.
Reviews for Recombinant Human MMP-9 Protein, CF
Average Rating: 4.6 (Based on 8 Reviews)
Have you used Recombinant Human MMP-9 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Reason for Rating: This enzyme was used to cleave a selection of FRET substrates for different MMPs and clearly cleaved the MMP9-specific peptide at the highest rate.
Reason for Rating: The product was easy to dissolve and use in treating human chondrocytes, exhibiting proper enzymatic activity in vitro