Recombinant Human MMP-7 Protein, CF

Catalog #: 907-MP
10 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
907-MP-010

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Citations (10)

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Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MMP-7 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂ (Catalog # ES001). The specific activity is >600 pmol/min/µg, as measured under the described conditions.

Source

Mouse myeloma cell line, NS0-derived human MMP-7 protein
Leu18-Lys267

Accession #

P09237

N-terminal Sequence
Analysis

Leu18

Structure / Form

Pro form

Predicted Molecular Mass

28 kDa & 20 kDa

SDS-PAGE

28 kDa (major) & 20 kDa (minor), reducing conditions

Product Datasheets

907-MP

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

907-MP

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl and CaCl₂.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

Materials

Assay Buffer: 50 mM Tris, 10 mM CaCl₂, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
Recombinant Human MMP-7 (rhMMP-7) (Catalog # 907-MP)
p-aminophenylmercuric acetate (APMA) (Sigma, Catalog # A-9563), 100 mM stock in DMSO
Substrate: MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH₂ (Catalog # ES001), 2 mM stock in DMSO
F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

Activate rhMMP-7 at 100 µg/mL with 1 mM APMA in Assay Buffer.
Incubate activation reaction at 37 °C for 1 hour.
Dilute activated rhMMP-7 to 0.4 ng/µL in Assay Buffer.
Dilute Substrate to 20 µM in Assay Buffer.
In a plate load 50 µL of 0.4 ng/µL rhMMP-7, and start the reaction by adding 50 µL of 20 µM Substrate to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate.
Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
Calculate specific activity:

Specific Activity (pmol/min/µg) =	Adjusted V_max* (RFU/min) x Conversion Factor** (pmol/RFU)
	amount of enzyme (µg)

*Adjusted for Substrate Blank

**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:

rhMMP-7: 0.02 µg
Substrate: 10 µM

Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Long Name

Matrix Metalloproteinase 7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Alternate Names

EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase

Related Research Areas

Citations for Recombinant Human MMP-7 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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A novel assay for improved detection of sputum periostin in patients with asthma
Authors: J Ono, M Takai, A Kamei, S Ohta, P Nair, K Izuhara, SE Dahlén, A James, BIOAIR con
PLoS ONE, 2023-02-10;18(2):e0281356.
Species: Human
Sample Types: Protein
Applications: Bioassay
Analysis of the inhibiting activity of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) on matrix metalloproteinases
Authors: SR Mendes, LD Amo-Maestr, L Marino-Pue, I Diego, T Goulas, FX Gomis-Rüth
Sci Rep, 2020-04-14;10(1):6317.
Species: Human
Sample Types: Protein
Applications: Bioassay
Novel Arginine-containing Macrocyclic MMP Inhibitors: Synthesis, 99mTc-labeling, and Evaluation
Authors: Y Ye, J Toczek, K Gona, HY Kim, J Han, M Razavian, R Golestani, J Zhang, TL Wu, M Ghosh, JJ Jung, MM Sadeghi
Sci Rep, 2018-08-03;8(1):11647.
Species: Human
Sample Types: Peptide
Applications: Enzyme Assay
Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer
Authors: N Willumsen, CL Bager, DJ Leeming, AC Bay-Jensen, MA Karsdal
Neoplasia, 2017-03-07;19(4):271-278.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay
Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Authors: SL King, HJ Joshi, KT Schjoldage, A Halim, TD Madsen, MH Dziegiel, A Woetmann, SY Vakhrushev, HH Wandall
Blood Adv, 2017-02-23;1(7):429-442.
Species: Human
Sample Types:
Applications: Bioassay
Serological assessment of neutrophil elastase activity on elastin during lung ECM remodeling.
Authors: Kristensen J, Karsdal M, Sand J, Willumsen N, Diefenbach C, Svensson B, Hagglund P, Oersnes-Leeming D
BMC Pulm Med, 2015-05-03;15(0):53.
Species: Human
Sample Types: Protein
Applications: Bioassay
Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers.
Authors: Djokic J, Fagotto-Kaufmann C, Bartels R, Nelea V, Reinhardt D
J Biol Chem, 2013-06-19;288(31):22821-35.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay
Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S
BJU Int., 2010-09-03;107(7):1069-73.
Applications: Western Blot
Determination of matrilysin activity in gastrointestinal neoplasia.
Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF
Eur. J. Clin. Invest., 2007-07-01;37(7):598-9.
Applications: ELISA (Standard)
Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006-07-01;10(1):39-50.
Species: Human
Sample Types:
Applications: Enzyme Assay

FAQs

Can the enzyme be stored after activation, or do I need to use it immediately after activation?
- We recommend only activating the amount of enzyme needed for your assay, and recommend activating the enzyme immediately prior to use. Any unactivated enzyme should be stored in aliquots at either the stock concentration at which the enzyme was supplied, or the reconstitution concentration, according to the product datasheet.
If I use this enzyme at a higher concentration, do I need to change the concentration of APMA to activate it?
- We have only optimized activation conditions for one particular concentration of this MMP enzyme as part of our regular QC testing for enzymatic activity. Activating the enzyme at any different concentration would have to be optimized by the end user.
Does this MMP enzyme need to be activated to work?
- Yes, this enzyme requires activation prior to use.
What is the activity of this enzyme in units/µg?
- We supply this enzyme as a mass and calculate its activity relative to mass (pmol/min/µg). We have not calibrated this enzyme to an international standard unit, so we are unable to provide a conversion to units/µg.