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Recombinant Human MIF Protein

Catalog #: 289-MF
31 Citations Datasheet
Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
289-MF-01M/CF
289-MF-002/CF

With Carrier

Catalog # Availability Size / Price Qty
289-MF-002
Recombinant Human MIF Protein SEC-MALS.
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Citations (31)
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Recombinant Human MIF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
E. coli-derived human MIF protein
Pro2-Ala115, with an N-terminal Met
Accession #
AAA36315
N-terminal Sequence
Analysis
Met1 & Pro2
Predicted Molecular Mass
12.4 kDa & 12.3 kDa

Product Datasheets

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289-MF (with carrier)

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289-MF/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

289-MF

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

289-MF/CF

Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

SEC-MALS View Larger

Recombinant Human MIF (Catalog # 289-MF) has a molecular weight (MW) of 32.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.

References
  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
  4. Kozak, C.A. et al. (1995) Genomics 27:405.
  5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
  6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
  7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
  8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
  9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
  11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
  12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
  13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Long Name
Macrophage Migration Inhibitory Factor
Entrez Gene IDs
4282 (Human); 17319 (Mouse)
Alternate Names
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

Citations for Recombinant Human MIF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Phosphorylation of MIF by PIP4K2a is necessary for cilia biogenesis
    Authors: Zhang, L;Zhang, H;Agborbesong, E;Zhou, JX;Li, X;
    Cell death & disease
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Involvement of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor in proinflammatory mechanisms promoting cardiovascular injury mechanisms promoting cardiovascular inflammation tThe combined action of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor increases proinflammatory mediator production by cardiomyocytes and vascular endothelial cells
    Authors: CS Rigazio, N Mariz-Pont, EP Caballero, FN Penas, NB Goren, MH Santamaría, RS Corral
    Microbial pathogenesis, 2022-11-12;0(0):105881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
    Authors: HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um
    Cells, 2020-05-28;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway
    Authors: H Chen, W Xia, M Hou
    Stem Cell Res Ther, 2020-01-21;11(1):31.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay
  5. Functional MIF promoter haplotypes modulate Th17-related cytokine expression in peripheral blood mononuclear cells from control subjects and rheumatoid arthritis patients
    Authors: LA Hernández-, S García-Are, R Bucala, MA Llamas-Cov, U De la Cruz, E Oregon-Rom, S Cerpa-Cruz, I Parra-Roja, A Plascencia, JF Muñoz-Vall
    Cytokine, 2018-11-19;115(0):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The macrophage migration inhibitory factor pathway in human B cells is tightly controlled and dysregulated in multiple sclerosis
    Authors: L Rijvers, MJ Melief, RM van der Vu, M Stéphant, J van Langel, AF Wierenga-W, JM Hogervorst, AJ Geurts-Moe, FCGJ Sweep, RQ Hintzen, MM van Luijn
    Eur. J. Immunol., 2018-09-25;0(0):.
    Species: Human
    Sample Types: Plasma
  7. Role of the Macrophage Migration Inhibitory Factor (MIF) in the survival of first trimester human placenta under induced stress conditions
    Authors: F Ietta, EAV Ferro, E Bevilacqua, L Benincasa, E Maioli, L Paulesu
    Sci Rep, 2018-08-14;8(1):12150.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection
    Authors: J Lang, Y Cheng, A Rolfe, C Hammack, D Vera, K Kyle, J Wang, TB Meissner, Y Ren, C Cowan, H Tang
    Stem Cell Reports, 2018-07-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
    Authors: JA Fraser, S Kemp, L Young, M Ross, M Prach, GR Hutchison, E Malone
    Sci Rep, 2018-05-14;8(1):7506.
    Applications: ELISA (Standard)
  10. Expression of Macrophage Migration Inhibitory Factor in Footpad Skin Lesions with Diabetic Neuropathy
    Authors: SU Noh, WY Lee, WS Kim, YT Lee, KJ Yoon
    Mol Pain, 2018-04-24;0(0):1744806918775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells
    Authors: S Sugita, K Makabe, Y Iwasaki, S Fujii, M Takahashi
    Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):1719-1731.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Novel half-life extended anti-MIF nanobodies protect against endotoxic shock
    Authors: A Sparkes, P De Baetsel, L Brys, I Cabrito, YG Sterckx, S Schoonoogh, S Muylderman, G Raes, R Bucala, P Vanlandsch, JA Van Ginder, B Stijlemans
    FASEB J., 2018-01-25;0(0):fj201701189R.
    Species: Human, Llama
    Sample Types: In Vivo, Recombinant Protein
    Applications: In Vivo, Surface Plasmon Resonance (SPR
  13. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
    Applications: Bioassay
  14. Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane
    Authors: R Kumar, T de Mooij, TE Peterson, T Kaptzan, AJ Johnson, DJ Daniels, IF Parney
    PLoS ONE, 2017-06-30;12(6):e0179012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. CYP19A1, MIF and ABCA1 genes are targets of the ROR? in monocyte and endothelial cells
    Authors: Neslihan Coban
    Cell Biol. Int, 2017-02-01;0(0):.
    Applications: ELISA (Standard)
  16. HIF1alpha-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN5.
    Authors: No Y, Lee S, Kumar A, Yun C
    PLoS ONE, 2015-09-09;10(9):e0137513.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. The expression of HIV-1 Vpu in monocytes causes increased secretion of TGF-beta that activates profibrogenic genes in hepatic stellate cells.
    Authors: Patel P, Khan N, Rani M, Gupta D, Jameel S
    PLoS ONE, 2014-02-13;9(2):e88934.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Tumor Microenvironment Macrophage Inhibitory Factor Directs the Accumulation of Interleukin-17-producing Tumor-infiltrating Lymphocytes and Predicts Favorable Survival in Nasopharyngeal Carcinoma Patients.
    Authors: Li J, Mo H, Xiong G, Zhang L, He J, Huang Z, Liu Z, Chen Q, Du Z, Zheng L, Qian C, Zeng Y
    J Biol Chem, 2012-08-14;287(42):35484-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
    Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
    Proc. Natl. Acad. Sci. U.S.A., 2011-07-05;108(29):12048-53.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Applications: ELISA (Standard)
  22. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
    Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
    FASEB J, 2010-02-12;24(7):2558-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Thrombin induced secretion of macrophage migration inhibitory factor (MIF) and its effect on nuclear signaling in endothelium.
    Authors: Wadgaonkar R, Somnay K, Garcia JG
    J. Cell. Biochem., 2008-12-01;105(5):1279-88.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor.
    Authors: Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS
    Endocrinology, 2008-07-24;149(11):5747-57.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2005-11-29;107(6):2252-61.
    Species: Human
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, ELISA (Standard)
  26. Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis.
    Authors: Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T
    Clin. Exp. Immunol., 2005-02-01;139(2):338-47.
    Applications: ELISA (Standard)
  27. Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
    Authors: Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V
    Biol. Reprod., 2004-11-24;72(4):949-53.
    Applications: Western Blot
  28. High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
    Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
    Crit. Care Med., 2004-03-01;32(3):734-9.
    Applications: ELISA (Standard)
  29. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor.
    Authors: Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T
    J. Clin. Invest., 2003-05-01;111(9):1309-18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy.
    Authors: Matsumoto K, 2019, Kanmatsuse K
    7587, 2002-10-01;92(2):309-15.
    Applications: ELISA (Standard)
  31. Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
    Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF
    J. Immunol., 2002-05-15;168(10):4889-96.
    Applications: ELISA (Standard)

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