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Recombinant Human LRP-1 Cluster II Fc Chimera Protein, CF

Catalog #: 2368-L2
7 Citations Datasheet
Catalog # Availability Size / Price Qty
2368-L2-050
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Citations (7)
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Recombinant Human LRP-1 Cluster II Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhLRP-1C2/Fc Chimera is immobilized at 50 ng/mL (100 µL/well), the concentration of rhLRPAP (Catalog # 4296-LR) that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LRP-1 Cluster II protein
Human LRP-1C2
(Arg786 - Leu1165)
Accession # Q07954		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
Q07954
N-terminal Sequence
Analysis
Arg786
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
68.1 kDa
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

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2368-L2

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2368-L2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1 Cluster II

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I transmembrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and the inhibition of serine proteases (1 - 4). LRP-1 also associates, or through intracellular scaffold proteins, with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled-1, various integrins, and the prion protein PrPC (1, 5 - 10). Human LRP 1 is N glycosylated and sialylated, and cleaved in the Golgi to produce an 85 kDa transmembrane beta chain, and a 515 kDa alpha chain. Both associate noncovalently, with the beta chain remaining completely extracellular (11, 12). The alpha chain of LRP 1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF-like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). Cluster II (aa 786 - 1165) contains one EGF-like and eight LDLR class A repeats (14, 15). Cluster II contains binding sites for Apolipoprotein E, LPL, LRPAP/RAP, alpha 2 Macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI-1, tPA-PAI-1 complexes, Pro-uPA, and TFPI (14, 15). Within this region, human LRP-1 shares 99% aa sequence identity with mouse and rat LRP-1. A shed soluble form of LRP-1 circulates in the serum and retains ligand binding properties (16).

References
  1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
  2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
  3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
  4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
  5. Takayama, Y. et al. (2005) J. Biol. Chem. 280:18504.
  6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
  7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
  10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
  11. Herz, J. et al. (1990) EMBO J. 9:1769.
  12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
  13. Herz, J. et al. (1988) EMBO J. 7:4119.
  14. Horn, I.R. et al. (1997) J. Biol. Chem. 272:13608.
  15. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
  16. Quinn, K.A. et al. (1999) Exp. Cell Res. 251:433.
Long Name
LDL Receptor-related Protein 1, Cluster II
Entrez Gene IDs
4035 (Human); 16971 (Mouse); 299858 (Rat)
Alternate Names
A2MR; A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5; Alpha-2-Macroglobulin Receptor; APOER; APR; CD91; LRP1 Cluster II; LRP-1 Cluster II; LRP1; TGFBR5

Citations for Recombinant Human LRP-1 Cluster II Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Generation of KS-487 as a novel LRP1-binding cyclic peptide with higher affinity, higher stability and BBB permeability
    Authors: K Sakamoto
    Biochemistry and Biophysics Reports, 2022-10-08;32(0):101367.
    Species: N/A
    Sample Types: Peptide
    Applications: ELISA Capture
  2. A novel peptide ligand RAP12 of LRP1 for glioma targeted drug delivery
    Authors: H Ruan, Z Chai, Q Shen, X Chen, B Su, C Xie, C Zhan, S Yao, H Wang, M Zhang, M Ying, W Lu
    J Control Release, 2018-04-19;0(0):.
    Species: Human
    Sample Types: Peptides
    Applications: Surface Plasmon Resonance (SPR
  3. Low-Density Lipoprotein Receptor-Related Protein-1 Is a Therapeutic Target in Acute�Myocardial Infarction
    Authors: S Toldo, D Austin, AG Mauro, E Mezzaroma, BW Van Tassel, C Marchetti, S Carbone, S Mogelsvang, C Gelber, A Abbate
    JACC Basic Transl Sci, 2017-10-30;2(5):561-574.
    Applications: Bioassay
  4. A novel LRP1-binding peptide L57 that crosses the blood brain barrier
    Authors: K Sakamoto, T Shinohara, Y Adachi, T Asami, T Ohtaki
    Biochem Biophys Rep, 2017-08-12;12(0):135-139.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Evidence that factor VIII forms a bivalent complex with the LDL receptor-related protein 1 (LRP1): Identification of cluster IV on LRP1 as the major binding site
    Authors: Dudley K Strickland
    J. Biol. Chem., 2016-10-29;0(0):.
    Applications: Bioassay
  6. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
    Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
    PLoS ONE, 2014-07-30;9(7):e103839.
    Applications: Bioassay
  7. A small molecule inhibitor to plasminogen activator inhibitor 1 inhibits macrophage migration.
    Authors: Ichimura A, Matsumoto S, Suzuki S, Dan T, Yamaki S, Sato Y, Kiyomoto H, Ishii N, Okada K, Matsuo O, Hou F, Vaughan D, van Ypersele de Strihou C, Miyata T
    Arterioscler Thromb Vasc Biol, 2013-03-07;33(5):935-42.
    Applications: Bioassay

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