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Recombinant Human KGF/FGF-7 Protein, CF

Catalog #: 11537-KG Datasheet
CHO expressed
Catalog # Availability Size / Price Qty
11537-KG-010
11537-KG-050
11537-KG-100
11537-KG-500
Recombinant Human KGF/FGF‑7 Protein Bioactivity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human KGF/FGF-7 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 4.00‑40.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human KGF/FGF-7 protein
Ser55-Thr194
Accession #
P21781.1
N-terminal Sequence
Analysis
Ser55
Predicted Molecular Mass
16 kDa
SDS-PAGE
15-18 kDa, under reducing conditions

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11537-KG

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11537-KG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute 10 μg size at 100 μg/mL and the other sizes at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 4.00-40.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # 11537-KG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-18 kDa, under reducing conditions.

Background: KGF/FGF-7

Fibroblast growth factor 7 (FGF7), also known as keratinocyte growth factor (KGF), is a member of the FGF family of secreted glycoproteins which are involved in key roles from development, morphogenesis, and angiogenesis to wound healing and tumorigenesis (1-4). FGF7, along with FGF3, FGF10, and FGF22, form a subfamily of the larger FGF family whose expression is restricted to cells of mesenchymal origin and function as paracrine growth factors for nearby epithelial cells (1). The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a conserved beta -trefoil structure (2). Mature human FGF7 shares 96% and 92% aa sequence identity with mouse and rat FGF7, respectively. FGF7 signals only through the IIIb splice form of the receptor FGFR2, whose expression is restricted primarily to epithelial cells (5). FGF7 is noticeably upregulated in response to damage to skin or internal structures and is proposed to help speed-up wound healing (6). Deletion of FGF7 affects kidney development, producing abnormally small ureteric buds and fewer nephrons (7). FGF7 has been proposed as a potential cancer therapeutic by targeting the intermolecular interaction site between FGF7 and FGFR2b (3). Additionally, the use of FGF7 is being explored as novel treatment options for cartilage diseases, such as Osteoarthritis and Rheumatoid arthritis (8). An N-terminally truncated version of FGF7 with improved thermal stability, named Palifermin (brand name Kepivance), is approved for treatment for reducing and/or healing damages caused by cancer chemotherapy and radiotherapy, such as severe oral mucositis (OM) (9,10).

References
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  3. Vadija, R. et al. (2016) J. Chem. Biol. 9:69.
  4. Itoh, N. and D.M. Ornitz (2004). Trends Genet. 20:563.
  5. Steiling, H. and S. Werner (2003) Curr. Opin. Biotechnol. 14:533.
  6. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  7. Qiao, J. et al. (1999) Development 126:547.
  8. Zhang, X. et al. (2023) Life Sci. 326:121804.
  9. Hsu, E. et al. (2006) PEDS 19:147.
  10. Sadeghi, S. et al. (2021)  Int. J. Biol. Macromol. 191:1175.
Long Name
Keratinocyte Growth Factor
Entrez Gene IDs
2252 (Human); 403915 (Canine)
Alternate Names
FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)

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