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Recombinant Human Jagged 1 Fc Chimera Protein, CF

Catalog #: 1277-JG
45 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
1277-JG-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (45)
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Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P78504
N-terminal Sequence
Analysis
Ser32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
137 kDa
SDS-PAGE
170-180 kDa, under reducing conditions

Product Datasheets

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1277-JG

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1277-JG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Citations for Recombinant Human Jagged 1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel
    Authors: Carolina, E;Kuse, Y;Okumura, A;Aoshima, K;Tadokoro, T;Matsumoto, S;Kanai, E;Okumura, T;Kasai, T;Yamabe, S;Nishikawa, Y;Yamaguchi, K;Furukawa, Y;Tanimizu, N;Taniguchi, H;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1
    Authors: Herrera, JL;Komatsu, M;
    Cell reports
    Species: Human
    Sample Types: Transduced Whole Cells, Transfected Whole Cells
    Applications: Bioassay
  3. A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells
    Authors: Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I;
    Stem cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Indirect Immobilised Jagged-1 Enhances Matrisome Proteins Associated with Osteogenic Differentiation of Human Dental Pulp Stem Cells: A Proteomic Study
    Authors: A Chansaenro, C Kornsuthis, S Roytrakul, S Phothichai, S Rochanavib, BPJ Fournier, SS Srithanyar, N Nowwarote, T Osathanon
    International Journal of Molecular Sciences, 2022-11-11;23(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Elevated ITGA5 facilitates hyperactivated mTORC1-mediated progression of laryngeal squamous cell carcinoma via upregulation of EFNB2
    Authors: D Li, A Sun, L Zhang, Z Ding, F Yi, X Yang, Z Wang, X Chen, W Liu, S Liu, H Shen, M Miao, L Zhang, P Liu, Y Liu, S Su, H Huang, C Huang, Z Hu, H Zhang, X Zha, Y Liu
    Theranostics, 2022-10-24;12(17):7431-7449.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells
    Authors: C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon
    Scientific Reports, 2022-05-09;12(1):7583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Human branching cholangiocyte organoids recapitulate functional bile duct formation
    Authors: FJM Roos, GS van Tiende, H Wu, I Bordeu, D Vinke, LM Albarinos, K Monfils, S Niesten, R Smits, J Willemse, O Rosmark, G Westergren, DJ Kunz, M de Wit, PJ French, L Vallier, JNM IJzermans, R Bartfai, H Marks, BD Simons, ME van Royen, MMA Verstegen, LJW van der La
    Cell Stem Cell, 2022-05-05;29(5):776-794.e13.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  8. Immobilization of Jagged1 Enhances Vascular Smooth Muscle Cells Maturation by Activating the Notch Pathway
    Authors: K Zohorsky, S Lin, K Mequanint
    Cells, 2021-08-14;10(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Sirt3 promotes the autophagy of HK?2 human proximal tubular epithelial cells via the inhibition of Notch?1/Hes?1 signaling
    Authors: Y Wang, J Chang, ZQ Wang, Y Li
    Molecular Medicine Reports, 2021-07-19;24(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Class II phosphatidylinositol 3-kinase-C2&alpha is essential for Notch signaling by regulating the endocytosis of &gamma-secretase in endothelial cells
    Authors: S Shimizu, K Yoshioka, S Aki, Y Takuwa
    Scientific Reports, 2021-03-04;11(1):5199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. miR-153 enhances the therapeutic effect of radiotherapy by targeting JAG1 in pancreatic cancer cells
    Authors: Z Zhao, X Shen, D Zhang, H Xiao, H Kong, B Yang, L Yang
    Oncology Letters, 2021-02-17;21(4):300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
    Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
    Cell Reports, 2020-12-15;33(11):108514.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  13. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells
    Authors: J Manokawinc, P Sumrejkanc, L Boonprakon, P Pavasant, H Egusa, T Osathanon
    Sci Rep, 2020-08-07;10(1):13329.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  15. Targeting the Notch and TGF-&beta signaling pathways to prevent retinal fibrosis in vitro and in vivo
    Authors: J Fan, W Shen, SR Lee, AE Mathai, R Zhang, G Xu, MC Gillies
    Theranostics, 2020-06-29;10(18):7956-7973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Notch signaling in mouse blastocyst development and hatching
    Authors: MR Batista, P Diniz, A Torres, D Murta, L Lopes-da-C, E Silva
    BMC Dev. Biol., 2020-06-02;20(1):9.
    Species: Mouse
    Sample Types: Embryo
    Applications: Bioassay
  17. Notch signalling drives synovial fibroblast identity and arthritis pathology
    Authors: K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner
    Nature, 2020-04-22;582(7811):259-264.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway
    Authors: SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies
    Front Cell Neurosci, 2019-12-03;13(0):527.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells
    Authors: M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer
    Oncogene, 2019-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Anti-Leukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia
    Authors: S Kannan, MJL Aitken, SM Herbrich, LS Golfman, MG Hall, DH Mak, JK Burks, G Song, M Konopleva, CG Mullighan, J Chandra, PA Zweidler-M
    Mol. Cancer Ther., 2019-06-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line
    Authors: S Matsui, M Ochiai, K Yasuda, SI Mae, M Kotaka, T Toyoda, T Yamamoto, K Osafune
    Stem Cell Res, 2019-01-31;35(0):101400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells
    Authors: S Stephenson, MA Care, I Fan, A Zougman, DR Westhead, GM Doody, RM Tooze
    J. Immunol., 2019-01-14;202(4):1287-1300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Notch signaling suppresses glucose metabolism in mesenchymal progenitors to restrict osteoblast differentiation
    Authors: SY Lee, F Long
    J. Clin. Invest., 2018-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells
    Authors: R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter
    J. Leukoc. Biol., 2018-10-08;105(1):101-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  25. RNA sequencing data of Notch ligand treated human dental pulp cells
    Authors: J Manokawinc, P Nattasit, T Thongngam, P Pavasant, KA Tompkins, H Egusa, T Osathanon
    Data Brief, 2018-01-31;17(0):407-413.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Sumoylation of Notch1 represses its target gene expression during cell stress
    Authors: CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren
    Cell Death Differ., 2018-01-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects
    Authors: DW Youngstrom, R Senos, RL Zondervan, JD Brodeur, AR Lints, DR Young, TL Mitchell, ME Moore, MH Myers, WJ Tseng, KM Loomes, KD Hankenson
    NPJ Regen Med, 2017-12-15;2(0):32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
    Authors: T Gocha, BM Rao, R DasGupta
    Sci Rep, 2017-09-20;7(1):12021.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration
    Authors: X Hong, A Margariti, A Le Bras, L Jacquet, W Kong, Y Hu, Q Xu
    Sci Rep, 2017-07-17;7(1):5590.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1? Upstream of Notch and SUMO Pathways
    Authors: M Ciria, NA García, I Ontoria-Ov, H González-K, R Carrero, JL De La Pomp, JA Montero, P Sepúlveda
    Stem Cells Dev., 2017-05-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration
    Authors: Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang
    Biochem. Biophys. Res. Commun., 2017-05-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Jagged1 Instructs Macrophage Differentiation in Leprosy
    PLoS Pathog, 2016-08-17;12(8):e1005808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.
    Authors: Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M
    PLoS ONE, 2016-01-28;11(1):e0148216.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015-08-24;17(9):1193-204.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Notch1 signaling regulates the epithelial-mesenchymal transition and invasion of breast cancer in a Slug-dependent manner.
    Authors: Shao, Shan, Zhao, Xiaoai, Zhang, Xiaojin, Luo, Minna, Zuo, Xiaoxiao, Huang, Shangke, Wang, Ying, Gu, Shanzhi, Zhao, Xinhan
    Mol Cancer, 2015-02-03;14(1):28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche.
    Authors: Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A
    J Transl Med, 2015-01-27;13(0):27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Notch1 stimulation induces a vascularization switch with pericyte-like cell differentiation of glioblastoma stem cells.
    Authors: Guichet P, Guelfi S, Teigell M, Hoppe L, Bakalara N, Bauchet L, Duffau H, Lamszus K, Rothhut B, Hugnot J
    Stem Cells, 2015-01-01;33(1):21-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
    Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
    Nat Commun, 2014-09-25;5(0):4806.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. 3D niche microarrays for systems-level analyses of cell fate.
    Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
    Nat Commun, 2014-07-14;5(0):4324.
    Applications: Bioassay
  40. Augmented osteogenic responses in human aortic valve cells exposed to oxLDL and TLR4 agonist: a mechanistic role of Notch1 and NF-kappaB interaction.
    Authors: Zeng Q, Song R, Ao L, Xu D, Venardos N, Fullerton D, Meng X
    PLoS ONE, 2014-05-08;9(5):e95400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Notch signaling is involved in neurogenic commitment of human periodontal ligament-derived mesenchymal stem cells.
    Authors: Osathanon T, Manokawinchoke J, Nowwarote N, Aguilar P, Palaga T, Pavasant P
    Stem Cells Dev, 2013-02-12;22(8):1220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012-07-16;181(3):1099-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012-07-09;7(7):e40490.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2011-07-26;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis.
    Authors: Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM
    Circulation, 2011-01-10;123(3):309-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human Jagged 1 Fc Chimera Protein, CF

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Recombinant Human Jagged 1 Fc Chimera Protein, CF
By Anonymous on 08/06/2021
Application: In vitro bioactivity in cell culture
Reason for Rating: It worked very good. The expression of p63 alpha in limbal stem cells was significantly reduced in the presence of Jag1 (5 µg/mL) in the cell culture medium.
In vitro bioactivity in cell culture Recombinant Human Jagged 1 Fc Chimera Protein, CF 1277-JG
Recombinant Human Jagged 1 Fc Chimera Protein, CF
By Anonymous on 04/04/2018
Application: In vitro bioactivity in cell culture
Reason for Rating: Good performance in Notch-reporter assay in cell lines
In vitro bioactivity in cell culture Recombinant Human Jagged 1 Fc Chimera Protein, CF 1277-JG