Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI1277 Datasheet

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI1277-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Notch‑1 Fc Chimera (Catalog # 3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein binds with an ED₅₀ of 0.500-2.50 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein

Human Jagged 1 (Ser32-Ser1046) Accession # P78504.3	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P78504.3

N-terminal Sequence
Analysis

Ser32

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

139 kDa

SDS-PAGE

150-170 kDa, under reducing conditions.

Product Datasheets

AVI1277

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1277

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Notch‑1 Fc Chimera (3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) binds with an ED50 of 0.500-2.50 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 150-170 kDa and 300-340 kDa, respectively.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10). Our Avi-tag Biotinylated Human Jagged 1 Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References