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Recombinant Human IL-17A (Human Cell-expressed) Protein

Catalog #: 7955-IL
16 Citations 3 Reviews Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
7955-IL-025/CF
7955-IL-010/CF
7955-IL-100/CF

With Carrier

Catalog # Availability Size / Price Qty
7955-IL-025
Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity
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Product Details
Citations (16)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A (Human Cell-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.12-1.2 ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155
Accession #
Q16552
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15.1 kDa (monomer)
SDS-PAGE
15-23 kDa, reducing conditions
28-38 kDa, non-reducing conditions

Product Datasheets

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7955-IL (with carrier)

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7955-IL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7955-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7955-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity View Larger

Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.12‑1.2 ng/mL.

Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17A shares 60% amino acid sequence identity with mouse and rat IL‑17A (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (16, 17).

References
  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  17. Kryczek, I. et al. (2009) Blood 114:357.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Alpha-synuclein fine-tunes neuronal response to pro-inflammatory cytokines
    Authors: Sigutova, V;Xiang, W;Regensburger, M;Winner, B;Prots, I;
    Brain, behavior, and immunity
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TNF-? and Poly(I:C) induction of A20 and activation of NF-?B signaling are independent of ABCF1 in human airway epithelial cells
    Authors: Cao, QT;Ishak, M;Shpilman, I;Hirota, JA;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease
    Authors: P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell
    Cell Reports, 2022-09-27;40(13):111439.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  4. Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L
    Authors: G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards
    BMC complementary medicine and therapies, 2022-05-12;22(1):131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway
    Authors: EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu
    Biomedicines, 2021-07-16;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Characterization of the interleukin-17 effect on articular cartilage in a translational model: an explorative study
    Authors: D Sinkeviciu, A Aspberg, Y He, AC Bay-Jensen, P Önnerfjord
    BMC Rheumatol, 2020-05-12;4(0):30.
    Species: Bovine
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network
    Authors: L Sun, M Girnary, L Wang, Y Jiao, E Zeng, K Mercer, J Zhang, JT Marchesan, N Yu, K Moss, YL Lei, S Offenbache, S Zhang
    J. Immunol., 2020-03-13;204(8):2177-2191.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. IL-36? regulates mediators of tissue homeostasis in epithelial cells
    Authors: JE Heath, GM Scholz, PD Veith, EC Reynolds
    Cytokine, 2019-03-08;119(0):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection
    Authors: S Muntaka, Y Almuhanna, D Jackson, S Singh, A Afryie-Asa, M Cámara, L Martínez-P
    Infect. Immun., 2019-01-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis
    Authors: J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston
    PLoS ONE, 2018-10-15;13(10):e0205340.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632
    Authors: ED Anderson, I Sastalla, NJ Earland, M Mahnaz, IN Moore, F Otaizo-Car, TG Myers, CA Myles, SK Datta, IA Myles
    PLoS ONE, 2018-09-12;13(9):e0198862.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Electrophilic properties of itaconate and derivatives regulate the�I?B?-ATF3 inflammatory axis
    Authors: M Bambouskov, L Gorvel, V Lampropoul, A Sergushich, E Loginichev, K Johnson, D Korenfeld, ME Mathyer, H Kim, LH Huang, D Duncan, H Bregman, A Keskin, A Santeford, RS Apte, R Sehgal, B Johnson, GK Amarasingh, MP Soares, T Satoh, S Akira, T Hai, C de Guzman, K Auclair, TP Roddy, SA Biller, M Jovanovic, E Klechevsky, KM Stewart, GJ Randolph, MN Artyomov
    Nature, 2018-04-18;556(7702):501-504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses
    Authors: BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas
    Vaccine, 2018-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  14. Population snapshots predict early haematopoietic and erythroid hierarchies
    Authors: BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky
    Nature, 2018-02-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature
    Authors: WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson
    Front Immunol, 2018-01-29;9(0):80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17
    PLoS ONE, 2016-09-02;11(9):e0161759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why? 

    • Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site  and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

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Reviews for Recombinant Human IL-17A (Human Cell-expressed) Protein

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Recombinant Human IL-17A (Human Cell-expressed) Protein
By Anonymous on 02/24/2021
Application: In vitro bioactivity in cell culture
Recombinant Human IL-17A (Human Cell-expressed) Protein
By Rachel Levantovsky on 11/18/2020
Application: In vitro bioactivity in cell culture
Recombinant Human IL-17A (Human Cell-expressed) Protein
By Anonymous on 06/04/2019
Application: CellProlif