Recombinant Human IL-17A/F Heterodimer Protein

Catalog #: 5194-IL
6 Citations 3 Reviews Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
5194-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
5194-IL-025

R&D Systems Recombinant Proteins and Enzymes

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All IL-17A/F Heterodimer Proteins and Enzymes

Product Details

Citations (6)

FAQs

Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A/F Heterodimer Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 3-15 ng/mL.

Source

E. coli-derived human IL-17A/F Heterodimer protein

Met	Human IL-17A (Ile20-Ala155) Accession # Q16552
Met	Human IL-17F (Arg31-Gln163) Accession # Q96PD4
N-terminus	C-terminus

Accession #

Q16552 (IL-17A) & Q96PD4 (IL-17F)

N-terminal Sequence
Analysis

Met (IL-17A) & Met (IL-17F)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

IL-17A: 15.7 kDa and IL-17F: 15 kDa

Product Datasheets

5194-IL (with carrier)

Product Datasheet

5194-IL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5194-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5194-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17A/F Heterodimer

Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Gu, C. et al. (2013) Cytokine 64:477.
Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
Ouyang, W. et al. (2008) Immunity 28:454.
Steinman, L. (2007) Nat. Med. 13:139.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.

Long Name

Interleukin 17 A/F

Alternate Names

CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A

Related Research Areas

Citations for Recombinant Human IL-17A/F Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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IL-17 cytokines preferentially act on naï¿½ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes
Authors: MP Crawford, N Borcherdin, NJ Karandikar
PLoS ONE, 2023-04-28;18(4):e0285166.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
IL-17 cytokines preferentially act on naï¿½ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes
Authors: MP Crawford, N Borcherdin, NJ Karandikar
PLoS ONE, 2023;18(4):e0285166.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
J Exp Med, 2015-04-27;212(5):619-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells.
Authors: Adams, Kelly M, Abraham, Valsamma, Spielman, Daniel, Kolls, Jay K, Rubenstein, Ronald C, Conner, Gregory, Cohen, Noam A, Kreindler, James L
PLoS ONE, 2014-08-20;9(8):e103263.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
J. Exp. Med., 2010-02-01;207(2):299-308.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)