Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

Catalog #: 5837-IL
1 Citation 2 Reviews Datasheet

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5837-IL-010

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All IL-17A/F Heterodimer Proteins and Enzymes

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Citations (1)

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Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.4‑2 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-17A/F Heterodimer protein

Human IL-17A
(Gly24-Ala155)
Accession # Q16552

Human IL-17F (Arg31-Gln163) Accession # Q96PD4
N-terminus	C-terminus

Accession #

Q16552 (IL-17A) & Q96PD4 (IL-17F)

N-terminal Sequence
Analysis

Gly24 (IL-17A) & Arg21 (IL-17F)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

15.1 kDa (IL-17A) & 14.9 kDa (IL-17F)

SDS-PAGE

20 & 16 kDa, reducing conditions

Product Datasheets

5837-IL

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5837-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17A/F Heterodimer

Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Gu, C. et al. (2013) Cytokine 64:477.
Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
Ouyang, W. et al. (2008) Immunity 28:454.
Steinman, L. (2007) Nat. Med. 13:139.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.

Long Name

Interleukin 17 A/F

Alternate Names

CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A

Related Research Areas

Citation for Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
Eur J Immunol, 2015-12-14;46(2):440-5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay