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Recombinant Human IL-17A Biotinylated Protein, CF

Catalog #: BT7955B
1 Citation Datasheet
Biotinylated
Catalog # Availability Size / Price Qty
BT7955B-025
Biotinylated Recombinant Human IL‑17/IL‑17A Protein Binding Activity.
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A Biotinylated Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL‑17/IL‑17A (Catalog # BT7955B) binds to Recombinant Human IL-17RA/IL-17R Fc Chimera Protein (Catalog # 177-IR) with a ED50 of 2.00-20.0 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155
Accession #
Q16552.1
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Biotinylated via amines
Predicted Molecular Mass
15.1 kDa
SDS-PAGE
12-25 kDa, under reducing conditions.

Product Datasheets

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BT7955B

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT7955B

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 100 µg/mL in sterile 4 mM HCL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL‑17/IL‑17A (Catalog # BT7955B) binds to Recombinant Human IL-17RA/IL-17R Fc Chimera Protein (177-IR) with a ED50 of 2.00‑20.0 ng/mL.

Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides
(9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

References
  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  17. Kryczek, I. et al. (2009) Blood 114:357.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Citation for Recombinant Human IL-17A Biotinylated Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification and structure-based drug design of cell-active inhibitors of interleukin 17A at a novel C-terminal site
    Authors: ER Goedken, MA Argiriadi, JD Dietrich, AM Petros, N Krishnan, SC Panchal, W Qiu, H Wu, H Zhu, AM Adams, PM Bodelle, L Goguen, PL Richardson, PF Slivka, M Srikumaran, AK Upadhyay, B Wu, RA Judge, A Vasudevan, SM Gopalakris, PB Cox, VS Stoll, C Sun
    Scientific Reports, 2022-08-26;12(1):14561.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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