Recombinant Human IGFBP-2 Protein, CF Summary
Product Specifications
Glu40-Gln328, with an N-terminal Met
Analysis
Product Datasheets
674-B2
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
674-B2
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IGFBP-2
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Human IGFBP-2 cDNA encodes a 328 amino acid (aa) residue precursor protein with a putative 39 aa residue signal peptide that is processed to generate the 289 aa residue mature protein. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2 - 10 fold higher affinity for IGF II than for IGF I.
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Citations for Recombinant Human IGFBP-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Multi-omic single-cell velocity models epigenome-transcriptome interactions and improves cell fate prediction
Authors: C Li, MC Virgilio, KL Collins, JD Welch
Nature Biotechnology, 2022-10-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in Mice
Authors: M Kaur, AC Drake, G Hu, S Rudnick, Q Chen, R Phennicie, R Attar, J Nemeth, F Gaudet, J Chen
J. Immunol., 2019-02-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Class 1-selective histone deacetylase inhibitors enhance HIV latency reversal while preserving the activity of HDAC isoforms necessary for maximal HIV gene expression
Authors: TD Zaikos, MM Painter, NT Sebastian, VH Terry, KL Collins
J. Virol., 2018-02-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs
Authors: E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich
Front Endocrinol (Lausanne), 2018-02-13;9(0):36.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Ex Vivo Expansion of CD34CD90CD49fHematopoietic Stem and Progenitor Cells from Non-enriched Umbilical Cord Blood with Azole Compounds
Authors: S Bari, Q Zhong, X Fan, Z Poon, AST Lim, TH Lim, N Dighe, S Li, GNC Chiu, CLL Chai, WYK Hwang
Stem Cells Transl Med, 2018-02-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
Authors: Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
Stem Cells, 2008-03-27;26(6):1628-35.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
Authors: Cortesio CL, Jiang W
Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
Species: Human
Sample Types: N/A
Applications: Enzyme Assay -
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay
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View all Proteins and Enzyme FAQsReviews for Recombinant Human IGFBP-2 Protein, CF
Average Rating: 5 (Based on 4 Reviews)
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Reason for Rating: Worked well with Quantikine ELISA.
Reason for Rating: We have found IGFBP-2 from other companies does not bind IGF-1. We have confirmed that this rIGFBP-2 from R and D binds IGF-1.
