Recombinant Human IGFBP-1 Protein, CF Summary
Product Specifications
Ala26-Asn259
Analysis
Product Datasheets
871-B1
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
871-B1
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IGFBP-1
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Human IGFBP-1 cDNA encodes a 259 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 234 aa residue mature protein. IGFBP-1 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. IGFBP-1 binds equally well to IGF-I and IGF-II, with phosphorylated forms of IGFBP-1 exhibiting higher binding affinities.
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Citations for Recombinant Human IGFBP-1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness
Authors: K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack
Nutrients, 2021-04-28;13(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
Authors: DH Bak, MJ Choi, SR Kim, BC Lee, JM Kim, ES Jeon, W Oh, ES Lim, BC Park, MJ Kim, J Na, BJ Kim
Korean J. Physiol. Pharmacol., 2018-08-27;22(5):555-566.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IGFBP1 increases ?-cell regeneration by promoting ?- to ?-cell transdifferentiation
EMBO J, 2016-08-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Biological variation in fasting serum insulin-like growth factor binding protein-1 (IGFBP-1) among individuals with a varying glucose tolerance.
Authors: Borai A, Livingstone C, Mehta S, Zarif H, Abdelaal F, Ferns G
Clin. Biochem., 2009-05-20;0(0):.
Applications: ELISA (Standard), Western Blot -
Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
Authors: Cortesio CL, Jiang W
Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
Species: Human
Sample Types: N/A
Applications: Enzyme Assay -
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay
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