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Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI720 Datasheet
Biotinylated
Catalog # Availability Size / Price Qty
AVI720-050
Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein Binding Activity
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Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Human ICAM-1 (14C11) Antibody (Catalog # MAB720) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag (Catalog # AVI720) binds with an ED50 of 3-18 ng/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.3-2.7 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human ICAM-1/CD54 protein
Human ICAM-1
(Gln28-Glu480)
Accession # P05362		IEGRMDHuman IgG1
(Pro100-Lys330)		GG Avi-tag
Accession #
P05362
N-terminal Sequence
Analysis

Gln28 inferred from enzymatic pyroglutamate treatment revealing Thr29

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
78 kDa
SDS-PAGE
95-125 kDa, under reducing conditions

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AVI720

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI720

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein Binding Activity View Larger

When Human ICAM-1/CD54 (Clone 14C11) Antibody (Catalog # MAB720) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag (Catalog # AVI720) binds with an ED50 of 3-18 ng/mL.

SDS-PAGE Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag (Catalog # AVI720) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-125 kDa and 195-215 kDa, respectively.

Background: ICAM-1/CD54

ICAM-1 (Intercellular Adhesion Molecule-1), also known as CD54, is a transmembrane cell adhesion glycoprotein and a member of the immunoglobulin supergene family. The ICAM sub-family consists of five members, ICAM-1 through ICAM-5, and they vary in their tissue expression and number of Ig-like domains in the extracellular domain (ECD). Full-length ICAM-1 contains five Ig-like domains in the ECD, a single transmembrane domain and short intracellular domain. Alternative splicing of ICAM-1 results in at least six membrane-bound forms and one soluble form (1). The ECD of mature, full-length human ICAM-1 shares 78% and 54% amino acid sequence identity with rat and mouse ICAM-1, respectively. ICAM-1 plays an important role in both innate and adaptive immune responses and is upregulated in endothelial and epithelial cells at sites of inflammation (2, 3). ICAM-1 mediates vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) 2, 3). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes (4-8). Soluble ICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage (9). Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria (10-18). More recently, ICAM-1 was shown to be a novel regulator of group 2 innate lymphoid cells (ILC2s) which play a key role in allergic airway inflammation (19).

References
  1. Robledo O. et al. (2003) Eur J Immunol. 33:1351.
  2. Springer T.A. (1994) Cell. 76:301.
  3. Yang, L. et al. (2005) Blood. 106:584.
  4. Rosenstein, Y. et al. (1991) Nature 354:233.
  5. Pluskota, E. and S.E. D’Souza (2000) Eur. J. Biochem. 267:4693.
  6. McCourt, P.A.G. et al. (1994) J. Biol. Chem. 269:30081.
  7. Kirchberger, S. et al. (2006) Immunobiology 211:537.
  8. Chakravorty S.J. and A. Craig (2005) Eur J. Cell Biol. 84:15.
  9. Ding, Y. et al. (2019) Ann. Clin. Transl. Neurol. 6:945.
  10. Benson, V. et al. (2007) Curr. Mol. Med. 7:219.
  11. Hoogeveen, R.C. et al. (2007) Diabetologia 50:36.
  12. Covarrubias, M. et al. (2007) Am. J. Transplant. 7:2573.
  13. Cottone, S. et al. (2007) J. Hypertens. 25:423.
  14. Brake, D.K. et al. (2006) Am. J. Physiol. Cell Physiol. 291:C1232.
  15. Madej, A. et al. (2005) Pharmacol. Rep. 57:878.
  16. El-Gohary, A.M. et al. (2004) Egypt. J. Immunol. 11:109.
  17. Christiansen, I. et al. (1996) Br. J. Haematol. 92:639.
  18. Kang, X. et al. (2005) World J. Gastroenterol. 11:4250.
  19. Lei, A. et al. (2018) JEM. 215:2157.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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