Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF Summary
Product Specifications
Glu49 - Glu460 with N-terminal 8-His and (DYKDDDDK) tags
Product Datasheets
SP-600
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
SP-600
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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1 μg per lane of Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460 (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
Background: BRD4
Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others. This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.
- Devaiah, B.N. et al. (2012) Proc. Natl. Acad. Sci. 109:6927.
- Gadd, M.S. et al. (2017) Nat. Chem. Biol. 13:514.
- Wang, R. et al. (2012) J. Biol. Chem. 287:10738.
- Zhang, W. et al. (1994) J. Biol. Chem. 287:43137.
Citation for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1
Authors: Meyers, M;Cismoski, S;Panidapu, A;Chie-Leon, B;Nomura, DK;
bioRxiv : the preprint server for biology
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
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