Recombinant Human Flt-3/Flk-2 Fc Chimera Protein Summary
Product Specifications
| Human Flt-3 (Asn27-Asn541) Accession # AAA18947 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
368-ST (with carrier)
368-ST/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
368-ST
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
368-ST/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Flt-3/Flk-2
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.
Citations for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain
Authors: A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat
Progress in neurobiology, 2023-01-13;0(0):102405.
Species: Human
Sample Types: Protein
Applications: Bioassay, SPR -
Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution
Authors: Q Jiang, J Isquith, L Ladel, A Mark, F Holm, C Mason, Y He, P Mondala, I Oliver, J Pham, W Ma, E Reynoso, S Ali, IJ Morris, R Diep, C Nasamran, G Xu, R Sasik, SB Rosenthal, A Birmingham, S Coso, G Pineda, L Crews, ME Donohoe, JC Venter, T Whisenant, RA Mesa, LB Alexandrov, KM Fisch, C Jamieson
Cell Reports, 2021-01-26;34(4):108670.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A systems biology pipeline identifies regulatory networks for stem cell engineering
Authors: MA Kinney, LT Vo, JM Frame, J Barragan, AJ Conway, S Li, KK Wong, JJ Collins, P Cahan, TE North, DA Lauffenbur, GQ Daley
Nat. Biotechnol., 2019-07-01;37(7):810-818.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation
Authors: Y Heshmati, S Kharazi, G Türköz, D Chang, E Kamali Dol, J Boström, A Krstic, T Boukoura, E Wagner, N Kadri, R Månsson, M Altun, H Qian, J Walfridsso
Sci Rep, 2018-07-25;8(1):11202.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34(+) cells.
Authors: Donahue RE, Wersto RP, Allay JA, Agricola BA, Metzger ME, Nienhuis AW, Persons DA, Sorrentino BP
Blood, 2000-01-15;95(2):445-52.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay
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