Recombinant Human Fibronectin Fragment 2 Protein, CF

Catalog #: 3225-FN Datasheet

Catalog #	Availability	Size / Price	Qty
3225-FN-100

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Recombinant Human Fibronectin Fragment 2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.

The ED₅₀for this effect is 0.25-1.25 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived human Fibronectin protein
Ser607-Pro1265, with a C-terminal 6-His tag

Accession #

NP_997647

N-terminal Sequence
Analysis

Ser607

Predicted Molecular Mass

73 kDa

SDS-PAGE

100 kDa, reducing conditions

Product Datasheets

3225-FN

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3225-FN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Fibronectin, encoded by the FN1 gene, is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed FN type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble fibronectin to fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

The recombinant human FN1.2 contains the first 7 FN type III domains (III1 - 7). III1 binds weakly to heparin and is thought to be important for self association and fibril formation. The recombinant human FN1.2 does not contain the known tripeptide RGD domain.

References