Recombinant Human Fibronectin, ACFP Protein Summary
Product Specifications
Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg/mL.
Optimal concentrations will need to be determined for individual user applications.
Met1-Pro1908, with a C-terminal 6-His tag
Produced in an animal component free process (ACFP).
Analysis
Product Datasheets
ACFP4305B (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
ACFP4305B
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and Tween®. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Fibronectin
Fibronectin (FN) is a large, modular glycoprotein that generates a polymeric fibrillar network in the extracellular matrix (ECM), and forms soluble, disulfide-linked dimeric protomers in plasma and other body fluids (1, 2). Fibronectin is a ligand for many molecules, including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrillar network. Conversion of soluble Fibronectin to Fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization (1). Fibronectin is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats (3-5). Alternative splicing generates multiple isoforms of Fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS) (5). Differential splicing within the IIICS domain determines the presence of CS1 and CS2 sequences, and its sensitivity to proteases (6, 7). The tilt angle between type III domains #9 and #10 (which contains an RGD motif) determines integrin binding affinity, suggesting how structural differences between fibrillar and soluble Fibronectin may influence their function (8). From the N-terminus to the furin cleavage site at amino acid 1908, human Fibronectin shares 92% amino acid sequence identity with mouse and rat Fibronectin.
- Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
- Potts, J.R. and I.D. Campbell (1996) Matrix Biol. 15:313.
- Bernard, M.P. et al. (1985) Biochemistry 24:2698.
- Kornblihtt, A.R. et al. (1983) Proc. Natl. Acad. Sci. USA 80:3218.
- Kornblihtt, A.R. et al. (1985) EMBO J. 4:1755.
- Mould, A.P. et al. (1991) J. Biol. Chem. 266:3579.
- Abe, Y. et al. (2005) Biochem. Biophys. Res. Commun. 338:1640.
- Altroff, H. et al. (2004) J. Biol. Chem. 279:55995.
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