Recombinant Human FGF basic Heat Stable Protein, Animal-Free New

Catalog #: BT-FGFBHS-AFL Datasheet

Catalog #	Availability	Size / Price	Qty
BT-FGFBHS-AFL-025
BT-FGFBHS-AFL-01M

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human FGF basic Heat Stable proteins.

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All FGF basic/FGF2/bFGF Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF basic Heat Stable Protein, Animal-Free Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.0500-0.600 ng/mL.

The specific activity of Animal-Free™ Recombinant Human FGF basic Heat Stable is >1.60 x 10⁶ IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Source

E. coli-derived human FGF basic/FGF2/bFGF protein
Ala135-Ser288 (with modifications)
Produced using non-animal reagents in an animal-free laboratory.

Accession #

NP_001997.5

N-terminal Sequence
Analysis

Ala135 & Ala136

Predicted Molecular Mass

17 kDa

SDS-PAGE

16-19 kDa, under reducing conditions.

Product Datasheets

BT-FGFBHS-AFL

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-FGFBHS-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Reconstitution	Reconstitute the 25 μg size at 250 μg/mL in sterile water. Reconstitute all other sizes at 500 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Equivalent bioactivity of GMP (BT-FGFBHS-GMP), Animal-Free (Catalog # BT-FGFBHS-AFL) and RUO (BT-FGFBHS) grades of Recombinant Human FGF basic Heat Stable proteins as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells (orange, green, red, respectively).

Bioactivity

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Animal-Free™ Recombinant Human FGF basic Heat Stable induces NR6R 3T3 mouse fibroblast cell proliferation. (A) Animal-Free™ Recombinant Human FGF basic Heat Stable (Catalog # BT-FGFBHS-AFL) or (B) wild type (WT) Recombinant Human FGF basic/FGF2/bFGF Proteins were untreated or incubated at 37°C for 3 days in media. Animal-Free™ Recombinant Human FGF basic Heat Stable protein retained similar activity to the untreated Heat Stable protein indicating that the Heat Stable protein has increased thermal stability. In contrast, WT Recombinant Human FGF basic/FGF2/bFGF Protein significantly lost activity suggesting less thermal stability.

SDS-PAGE

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2 μg/lane of Animal-Free™ Recombinant Human FGF basic Heat Stable Protein (Catalog # BT-FGFBHS-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-19 kDa, under reducing conditions.

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules. As a growth factor, FGF basic is expressed in a variety of cell types and involved in the regulation of cell proliferation, differentiation, migration and survival. FGF basic promotes self-renewal and upregulates pluripotency markers such as Oct4, Sox2 and Nanog, making it well-known for maintenance of pluripotency in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). These functions make FGF basic an important cell culture supplement in stem cell applications for regenerative medicine and clinical manufacturing protocols.

Our Heat Stable FGF basic has been engineered for thermostability and maintains its activity at 37℃ when compared to wild-type. Heat-stable FGF basic can enhance the flexibility and efficiency of cell culture by reducing the need for frequent media changes.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement