Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein

Catalog #: 232-FA
22 Citations 1 Review Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
232-FA-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
232-FA-025

R&D Systems Recombinant Proteins and Enzymes

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All FGF acidic/FGF1 Proteins and Enzymes

Product Details

Citations (22)

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is 0.015-0.15 ng/mL in the presence of 10 µg/mL of heparin.

Source

E. coli-derived human FGF acidic/FGF1 protein
Phe16-Asp155, with an N-terminal Met

Accession #

NP_000791

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

15.5 kDa

Product Datasheets

232-FA (with carrier)

Product Datasheet

232-FA/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

232-FA

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

232-FA/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).

References

Jaye, M. et al. (1986) Science 233:541.
Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.
Rajalingam, D. et al. (2007) Biochemistry 46:9225.
Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
Imamura, T. et al. (1990) Science 249:1567.
Wesche, J. et al. (2005) Biochemistry 44:6071.
Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
Bouleau, S. et al. (2005) Oncogene 24:7839.

Long Name

Fibroblast Growth Factor acidic

Entrez Gene IDs

2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

Alternate Names

AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1

Related Research Areas

Citations for Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Estrogen regulation and functional role of FGFR4 in estrogen receptor positive breast cancer
Authors: Ding, K;Chen, L;Levine, K;Sikora, M;Tasdemir, N;Dabbs, D;Jankowitz, R;Hazan, R;Shah, OS;Atkinson, JM;Lee, AV;Oesterreich, S;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of Autophagy
Authors: Huang, HW;Yang, CM;Yang, CH;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Localized astrogenesis regulates gyrification of the cerebral cortex
Authors: Y Shinmyo, K Saito, T Hamabe-Hor, N Kameya, A Ando, K Kawasaki, TAD Duong, M Sakashita, J Roboon, T Hattori, T Kannon, K Hosomichi, M Slezak, MG Holt, A Tajima, O Hori, H Kawasaki
Science Advances, 2022-03-11;8(10):eabi5209.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Fibroblast Growth Factor Type 1 Ameliorates High-Glucose-Induced Oxidative Stress and Neuroinflammation in Retinal Pigment Epithelial Cells and a Streptozotocin-Induced Diabetic Rat Model
Authors: HW Huang, CM Yang, CH Yang
International Journal of Molecular Sciences, 2021-07-05;22(13):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FGF signal is not required for hepatoblast differentiation of human iPS cells
Authors: Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi
Sci Rep, 2019-03-06;9(1):3713.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Higher-Order Kidney Organogenesis from Pluripotent Stem Cells
Authors: A Taguchi, R Nishinakam
Cell Stem Cell, 2017-11-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
Authors: Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J
Nat Med, 2015-04-20;21(5):530-6.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
Authors: Tucker, Budd A, Mullins, Robert F, Streb, Luan M, Anfinson, Kristin, Eyestone, Mari E, Kaalberg, Emily, Riker, Megan J, Drack, Arlene V, Braun, Terry A, Stone, Edwin M
Elife, 2013-08-27;2(0):e00824.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation.
Authors: Tucker B, Anfinson K, Mullins R, Stone E, Young M
Stem Cells Transl Med, 2012-12-27;2(1):16-24.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Identification of BMP and activin membrane-bound inhibitor (BAMBI) as a potent negative regulator of adipogenesis and modulator of autocrine/paracrine adipogenic factors.
Authors: Luo X, Hutley LJ, Webster JA, Kim YH, Liu DF, Newell FS, Widberg CH, Bachmann A, Turner N, Schmitz-Peiffer C, Prins JB, Yang GS, Whitehead JP
Diabetes, 2012-01-01;61(1):124-36.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
PLoS ONE, 2010-08-11;5(8):e12101.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Similar expression to FGF (Sef) inhibits fibroblast growth factor-induced tumourigenic behaviour in prostate cancer cells and is downregulated in aggressive clinical disease.
Authors: Darby S, Murphy T, Thomas H, Robson CN, Leung HY, Mathers ME, Gnanapragasam VJ
Br. J. Cancer, 2009-11-03;101(11):1891-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
Stem Cells, 2008-05-15;26(7):1778-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Specific heparan sulfate structures modulate FGF10-mediated submandibular gland epithelial morphogenesis and differentiation.
Authors: Patel VN, Likar KM, Zisman-Rozen S, Cowherd SN, Lassiter KS, Sher I, Yates EA, Turnbull JE, Ron D, Hoffman MP
J. Biol. Chem., 2008-01-28;283(14):9308-17.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Heparanase cleavage of perlecan heparan sulfate modulates FGF10 activity during ex vivo submandibular gland branching morphogenesis.
Authors: Patel VN, Knox SM, Likar KM, Lathrop CA, Hossain R, Eftekhari S, Whitelock JM, Elkin M, Vlodavsky I, Hoffman MP
Development, 2007-10-24;134(23):4177-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Liver-specific activities of FGF19 require Klotho beta.
Authors: Lin BC, Wang M, Blackmore C, Desnoyers LR
J. Biol. Chem., 2007-07-11;282(37):27277-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma.
Authors: Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK
Blood, 2006-07-18;108(10):3465-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro.
Authors: Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A
Int. J. Dev. Biol., 2006-01-01;50(7):645-52.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis.
Authors: Shin JW, Min M, Larrieu-Lahargue F, Canron X, Kunstfeld R, Nguyen L, Henderson JE, Bikfalvi A, Detmar M, Hong YK
Mol. Biol. Cell, 2005-11-16;17(2):576-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
Development, 2005-02-16;132(6):1223-34.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Liver tissue engineering at extrahepatic sites in mice as a potential new therapy for genetic liver diseases.
Authors: Ohashi K, Waugh JM, Dake MD, Yokoyama T, Kuge H, Nakajima Y, Yamanouchi M, Naka H, Yoshioka A, Kay MA
Hepatology, 2005-01-01;41(1):132-40.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries.
Authors: Dame MK, Yu X, Garrido R, Bobrowski W, McDuffie JE, Murphy HS, Albassam M, Varani J
In Vitro Cell. Dev. Biol. Anim., 2003-11-01;39(10):402-6.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay