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Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93)

Catalog #: 351-FS
9 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
351-FS-010/CF
351-FS-050/CF

With Carrier

Catalog # Availability Size / Price Qty
351-FS-010
351-FS-050
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Citations (9)
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Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93) Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 10-30 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.3-1.5 ng/mL.
Source
E. coli-derived CXCL12/SDF-1 beta protein
Lys22-Met93
Accession #
P48061
N-terminal Sequence
Analysis
Lys22
Predicted Molecular Mass
8 kDa

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351-FS (with carrier)

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351-FS/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

351-FS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

351-FS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1 beta

CXCL12, also known as SCYB12, PBSF and SDF-1 beta, is an 8.3 kDa, heparin-binding member of the CXC (or alpha-) family of chemokines (1, 2). Feline CXCL12( beta ) is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region (3). The mature molecule exhibits a typical three antiparallel beta -strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa’s 1-8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation (4). The C-terminus is likely associated with heparin binding (5). SDF-1 beta circulates and undergoes proteolytic processing. CD26 will remove the first two N-terminal amino acids, possibly creating a reduced-activity chemokine (5, 6). In addition to the beta -isoform, alternate splicing of the feline SDF-1 gene generates an alpha -isoform. The alpha isoform is identical to SDF-1 beta, but shorter by four aa’s at the C-terminus (3). Although alpha - and beta -isoforms show similar activity, SDF-1 alpha is differentially processed, and different cells secrete the two isoforms (5, 7). Mature feline SDF-1 beta is 96%, 97% and 100% aa identical to rat, mouse and human SDF-1 beta, respectively. Human (and by inference, feline) SDF-1 is active on mouse cells. SDF-1 alpha and beta are reported to be monomers at neutral pH and physiologic ionic strength (4). SDF-1 alpha is also reported to form dimers in the presence of heparansulfate (8). On the cell surface, this may well facilitate SDF-1 interaction with its two receptors, CXCR4 and syndecan-4 (9). Heparin sulfate is known to protect SDF-1 from proteolysis, and CXCR4 exists constitutively as a dimer (9-11). Among its many functions, CXCL12 is known to influence lymphopoiesis, regulate patterning and cell number of neural progenitors, and promote angiogenesis (12, 13). It also enhances the survival of myeloid progenitor cells.

References
  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Rollins, B.J. (1997) Blood 90:909.
  3. Nishimura, Y. et al. (1998) Eur. J. Immunogenet. 25:303.
  4. Crump, M.P. et al. (1997) EMBO J. 16:6996.
  5. Sierra, M.D. L. et al. (2004) Blood 103:2452.
  6. Davis, D.A. et al. (2005) Blood 105:4561.
  7. Stumm, R.K. et al. (2002) J. Neurosci. 22:5865.
  8. Veldkamp, C.T. et al. (2005) Protein Sci. 14:1071.
  9. Charnaux, N. et al. (2005) FEBS J. 272:1937.
  10. Percherancier, Y. et al. (2005) J. Biol. Chem. 280:9895.
  11. Babcock, G.J. et al. (2003) J. Biol. Chem. 278:3378.
  12. Klein, R.S. et al. (2004) Trends Immunol. 25:306.
  13. Salcedo, R. and J.J. Oppenheim (2003) Microcirculation 10:359.
  14. Broxmeyer, H.E. et al. (2003) J. Leukoc. Biol. 73:630.
Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline)
Alternate Names
CXCL12/SDF-1 beta; hSDF-1beta; SDF 1beta; SDF1 beta; SDF1b

Citations for Recombinant Human/Feline CXCL12/SDF-1 beta (aa 22-93)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
    Authors: J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann
    Cell Stem Cell, 2021-12-25;29(2):217-231.e8.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  2. Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway
    Authors: J Lim, J Heo, H Ju, JW Shin, Y Kim, S Lee, HY Yu, CM Ryu, H Yun, S Song, KS Hong, HM Chung, HR Kim, JS Roe, K Choi, IG Kim, EM Jeong, DM Shin
    Sci Adv, 2020-04-15;6(16):eaba1334.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Stimulation of chondrocyte hypertrophy by chemokine stromal cell-derived factor 1 in the chondro-osseous junction during endochondral bone formation.
    Authors: Wei L, Kanbe K, Lee M, Wei X, Pei M, Sun X, Terek R, Chen Q
    Dev. Biol., 2010-03-04;341(1):236-45.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Applications: ELISA (Standard)
  6. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Applications: ELISA (Standard)
  7. A neurovascular niche for neurogenesis after stroke.
    Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
    J. Neurosci., 2006-12-13;26(50):13007-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006-05-01;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001-04-01;31(4):1037-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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