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Recombinant Human ErbB2/Her2 His-tag Protein, CF

Catalog #: 10126-ER
3 Citations Datasheet
Catalog # Availability Size / Price Qty
10126-ER-050
Recombinant Human ErbB2/Her2 His-tag Protein Bioactivity
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Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ErbB2/Her2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is 0.3-1.2 μg/mL in the presence of 0.6 µg/mL Human ErbB2/Her2 Antibody (Trastuzumab, Catalog # MAB9589).
Source
Human embryonic kidney cell, HEK293-derived human ErbB2/Her2 protein
Ser22-Thr652, with a C-terminal 6-His tag
Accession #
P04626-1
N-terminal Sequence
Analysis
Ser22
Predicted Molecular Mass
70 kDa
SDS-PAGE
93-108 kDa, under reducing conditions

Product Datasheets

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10126-ER

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10126-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human ErbB2/Her2 His-tag Protein Bioactivity View Larger

Recombinant Human ErbB2/Her2 His-tag (Catalog # 10126-ER) blocks anti-hErbB2 antibody (Trastuzumab, Catalog # MAB9589) mediated inhibition of the SK-BR-3 human breast cancer cell line proliferation. The ED50 is 0.3-1.2 µg/mL in the presence of 0.6 µg/mL antibody (Trastuzumab, Catalog # MAB9589).

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain.  Within the ECD, human ErbB2 shares and 86% aa sequence identity with mouse and rat ErbB2.  Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Recombinant Human ErbB2/Her2 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models
    Authors: Y Yang, H Yang, Y Alcaina, J Puc, A Birt, Y Vedvyas, M Gallagher, S Alla, MC Riascos, JE McCloskey, K Du, J Gonzalez-V, IM Min, E de Stanchi, M Britz, E von Hofe, MM Jin
    Nature Communications, 2023-04-12;14(1):2068.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Spatial-Division Multiplexing Approach for Simultaneous Detection of Fiber-Optic Ball Resonator Sensors: Applications for Refractometers and Biosensors
    Authors: M Shaimerden, T Ayupova, A Bekmurzaye, M Sypabekova, Z Ashikbayev, D Tosi
    Biosensors, 2022-11-11;12(11):.
    Species: Human
    Sample Types: Serum
    Applications: Bioassay
  3. An antibody Fc engineered for conditional antibody-dependent cellular cytotoxicity at the low tumor microenvironment pH using mammalian cell display
    Authors: Y Liu, AG Lee, AW Nguyen, JA Maynard
    The Journal of Biological Chemistry, 2022-03-03;0(0):101798.
    Species: Human, Transgenic Mouse
    Sample Types: Serum, Whole Cells
    Applications: ELISA Capture, Flow Cytometry

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