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Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF

Catalog #: AVI11356 Datasheet
Catalog # Availability Size / Price Qty
AVI11356-050
Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein Binding Activity.
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Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (Catalog # MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag (Catalog # AVI11356) binds with an ED50 of 0.500-6.00 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ErbB2/Her2 protein
Human ErbB2
(Thr23-Thr652)
Accession # P04626.1		Avi-tag6-His tag
N-terminusC-terminus
Accession #
P04626.1
N-terminal Sequence
Analysis
Thr23
Predicted Molecular Mass
73 kDa
SDS-PAGE
92-112 kDa, under reducing conditions.

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AVI11356

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11356

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein (Catalog # AVI11356) binds with an ED50 of 0.500‑6.00 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein (Catalog # AVI11356) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-112 kDa.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain.  Within the ECD, human ErbB2 shares and 86% aa sequence identity with mouse and rat ErbB2.  Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10). Our Avi-tag Biotinylated human ErbB2/Her2 His-tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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