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Recombinant Human EphA1 Fc Chimera Protein, CF

Catalog #: 7146-A1
2 Citations Datasheet
Catalog # Availability Size / Price Qty
7146-A1-100
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Recombinant Human EphA1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human EphA1 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Biotinylayed Recombinant Mouse Ephrin‑A1 Fc Chimera (Catalog # BT602) that produces 50% of the optimal binding response is typically 10-50 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human EphA1 protein
Human EphA1
(Met1-Glu547)
Accession # AAA36747		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
AAA36747
N-terminal Sequence
Analysis
Lys26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
83.5 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

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7146-A1

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7146-A1

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, PEG 3350, CHAPS and Imidazole.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: EphA1

EphA1, also known as Eph and Esk, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1‑3). Mature mouse EphA1 consists of a 524 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (4). Within the ECD, human EphA1 shares 84% aa sequence identity with mouse and rat EphA1. EphA1 can form pH sensitive cis-homodimers on the cell surface (5). Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (6). Reverse signaling is propagated through the Ephrin ligand (7). EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (6, 8). It is additionally expressed on CD4+ T cells and a subpopulation of CD19+ B cells (9, 10). On CD4+ T cells, EphA1 interacts with EphA4, induces Tyr phosphorylation of PYK2, and promotes chemokine-induced chemotaxis (9, 10). EphA1 is up‑regulated or down‑regulated in a variety of human carcinomas and is implicated in tumor invasiveness (3, 7, 11). The region of Fibronectin including type I repeats #10‑12 interacts with EphA1, and this interaction plays a role in VEGF-dependent in vitro angiogenesis (12).

References
  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  3. Mosch, B. et al. (2010) J. Oncol. Mar 10 Epub.
  4. Hirai H. et al. (1987) Science 238:1717.
  5. Bocharov, E.V. et al. (2008) J. Biol. Chem. 283:29385.
  6. Douville, E.M.J. et al. (1992) Mol. Cell. Biol. 12:2681.
  7. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  8. Coulthard, M.G. et al. (2001) Growth Factors 18:303.
  9. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  10. Holen, H.L. et al. (2010) J. Leukoc. Biol. 87:1059.
  11. Dong, Y. et al. (2009) Mod. Pathol. 22:151.
  12. Masuda, J. et al. (2008) J. Biol. Chem. 283:13148.
Entrez Gene IDs
2041 (Human); 13835 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; EPH receptor A1; eph tyrosine kinase 1; EphA1; EPHephrin type-A receptor 1; EPHT; EPHT1erythropoietin-producing hepatoma amplified sequence; Esk; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH

Citations for Recombinant Human EphA1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: N/A
    Sample Types: Protein
    Applications: ELISA Capture
  2. ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
    Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
    Oncogene, 2013-05-20;33(17):2179-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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