Recombinant Human Endostatin Protein, CF Summary
Product Specifications
His1154-Ser1335, with an N-terminal Met and 8-His tag
Analysis
Product Datasheets
1098-ES
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1098-ES
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Endostatin
Endostatin, an endogenous non‑glycosylated inhibitor of endothelial cell proliferation and angiogenesis, is an approximately 181 amino acid (aa), 20 kDa proteolytic fragment of the C‑terminal non‑collagenous domain of type XVIII collagen (1, 2). It is produced and/or trimmed by metalloproteinases such as MMP‑2 and MMP‑9, and cathepsins S, B and L (3, 4). The N‑terminal ~27 aa of Endostatin appear to contain the majority of its activity (4, 5). This region contains three zinc binding sites that are thought to be critical for its anti‑endothelial and anti‑tumor effects, as well as multiple cleavage sites that, when used, can modify its activity (4, 5). Human Endostatin shares 84‑87% aa sequence identity with mouse, rat, bovine and equine Endostatin. Endostatin inhibits endothelial cell growth by inducing cell cycle arrest in G1 phase and initiating apoptosis (1, 2). It is also thought to down‑regulate angiogenesis by blocking VEGF‑induced endothelial cell migration (6, 7). It alters the effect of FGF basic on adhesion and cell motility (8). Endostatin can interact with Transglutaminase 2, heparin, and integrins alpha 5 beta 1 and alpha v beta 3, all of which may be secreted by, or expressed on, endothelial cells, and can influence adhesion or migration (9, 10). Endostatin may also be involved with down‑regulation of angiogenesis after establishment of placental circulation in the pregnant uterus (11). Over‑expression in keratinocytes causes delay in healing of excisional wounds, while exogenous systemic Endostatin promotes endothelial and smooth muscle nitric oxide production and decreases blood pressure (12, 13). Since tumor growth and metastasis relies on angiogenesis to provide blood supply, Endostatin is inhibitory for a wide variety of primary and metastatic tumors (5‑8).
- Dhanabal, M. et al. (1999) Biochem. Biophys. Res. Commun. 10:345.
- O’Reilly, M.S. et al. (1997) Cell 88:277.
- Nilsson, U.W. et al. (2006) Cancer Res. 66:4789.
- Veillard, F. et al. (2011) J. Biol. Chem. 286:37158.
- Folkman, J. (2006) Exp. Cell Res. 312:594.
- Yamaguchi, N. et al. (2001) EMBO J. 18:4414.
- Ling, Y. et al. (2007) Biochem. Biophys. Res. Commun. 361:79.
- Dixelius, J. et al. (2002) Cancer Res. 62:1944.
- Faye, C. et al. (2010) Biochem. J. 427:467.
- Rehn, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:1024.
- Pollheimer, J. et al. (2011) Endocrinol. 152:4431.
- Seppinen, L. et al. (2008) Matrix Biol. 27:535.
- Sunshine, S.B. et al. (2012) Proc. Natl. Acad. Sci. USA 109:11306.
Citations for Recombinant Human Endostatin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Endostatin in 3D Fibrin Hydrogel Scaffolds Promotes Chondrogenic Differentiation in Swine Neonatal Meniscal Cells
Authors: VR Herrera Mi, B Canciani, L Mangiavini, JFS Filipe, L Aidos, M Pallaoro, GM Peretti, P Pocar, SC Modina, A Di Giancam
Biomedicines, 2022-09-27;10(10):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Collagen 18 and agrin are secreted by enteric neural crest cells to remodel their microenvironment and regulate their migration during ENS development
Authors: N Nagy, C Barad, R Hotta, S Bhave, E Arciero, D Dora, AM Goldstein
Development, 2018-05-08;0(0):.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay
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