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Recombinant Human Endoglin/CD105 Protein

Catalog #: 1097-EN
11 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
1097-EN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1097-EN-025
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Citations (11)
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Recombinant Human Endoglin/CD105 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.06-0.36 μg/mL in the presence of 100 ng/mL of Recombinant Human BMP‑10 (Catalog # 2926-BP).
Source
Mouse myeloma cell line, NS0-derived human Endoglin/CD105 protein
Glu26-Gly586
Accession #
Q5T9C0
N-terminal Sequence
Analysis
Glu26
Predicted Molecular Mass
61 kDa
SDS-PAGE
75-85 kDa, reducing conditions

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1097-EN (with carrier)

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1097-EN/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1097-EN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1097-EN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2 ‑ 5). Human endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two‑part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S‑endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65‑72% aa identity with mouse, rat, bovine, porcine and canine endoglin.  Endoglin homodimers interact with TGF‑ beta 1 and TGF‑ beta 3 (but not TGF‑ beta 2), but only after binding T beta RII (8). Similarly, they interact with activin‑A and BMP‑7 via activin type IIA or B receptors, and with BMP‑2 via BMPR‑1A/ALK‑3 or BMPR‑1B/ALK‑6 (9). BMP‑9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand‑induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF‑ beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1‑mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14).  In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

Citations for Recombinant Human Endoglin/CD105 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Regulation and Release of Vasoactive Endoglin by Brain Endothelium in Response to Hypoxia/Reoxygenation in Stroke
    Authors: A Haarmann, L Zimmermann, M Bieber, C Silwedel, G Stoll, MK Schuhmann
    International Journal of Molecular Sciences, 2022-06-25;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble Endoglin Stimulates Inflammatory and Angiogenic Responses in Microglia That Are Associated with Endothelial Dysfunction
    Authors: ES Park, S Kim, DC Yao, JPJ Savarraj, HA Choi, PR Chen, E Kim
    International Journal of Molecular Sciences, 2022-01-22;23(3):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  3. Pregnancy-Induced High Plasma Levels of Soluble Endoglin in Mice Lead to Preeclampsia Symptoms and Placental Abnormalities
    Authors: L Pérez-Roqu, E Núñez-Góme, A Rodríguez-, C Bernabéu, JM López-Novo, M Pericacho
    International Journal of Molecular Sciences, 2020-12-26;22(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Potential Role of Circulating Endoglin in Hypertension via the Upregulated Expression of BMP4
    Authors: E Gallardo-V, L Gamella-Po, L Perez-Roqu, JL Bartha, I Garcia-Pal, JI Casal, JM López-Novo, M Pericacho, C Bernabeu
    Cells, 2020-04-16;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling
    Authors: L Pous, SS Deshpande, S Nath, S Mezey, SC Malik, S Schildge, C Bohrer, K Topp, D Pfeifer, F Fernández-, S Doostkam, DK Galanakis, V Taylor, K Akassoglou, C Schachtrup
    Nat Commun, 2020-01-31;11(1):630.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners
    Authors: E Gallardo-V, L Ruiz-Llore, J Casado-Vel, MJ Ruiz-Rodrí, N López-Andr, AK Pattnaik, M Quintanill, C Bernabeu
    Cells, 2019-09-13;8(9):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay, Bio-Layer Interferometry (BLI)
  7. Microglial Phenotyping in Neurodegenerative Disease Brains: Identification of Reactive Microglia with an Antibody to Variant of CD105/Endoglin
    Authors: DG Walker, LF Lue, TG Beach, I Tooyama
    Cells, 2019-07-23;8(7):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  8. Circulating soluble endoglin modifies the inflammatory response in mice
    Authors: L Ruiz-Remol, C Ollauri-Ib, L Pérez-Roqu, E Núñez-Góme, F Pérez-Barr, JM López-Novo, M Pericacho, A Rodríguez-
    PLoS ONE, 2017-11-16;12(11):e0188204.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin.
    Authors: Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T
    Blood Cancer J, 2014-03-21;4(0):e196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Endothelial endoglin is involved in inflammation: role in leukocyte adhesion and transmigration.
    Authors: Rossi E, Sanz-Rodriguez F, Eleno N, Duwell A, Blanco F, Langa C, Botella L, Cabanas C, Lopez-Novoa J, Bernabeu C
    Blood, 2012-10-16;121(2):403-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Matrix metalloproteinase-14 (MT1-MMP)-mediated endoglin shedding inhibits tumor angiogenesis.
    Authors: Hawinkels LJ, Kuiper P, Wiercinska E
    Cancer Res., 2010-04-27;70(10):4141-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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