Recombinant Human EGFR Protein

Catalog #: 1095-ER
5 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
1095-ER-002

R&D Systems Recombinant Proteins and Enzymes

1 Image

All EGFR Proteins and Enzymes

Product Details

Citations (5)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EGFR Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human EGFR protein
Met1-Ser645

Accession #

CAA25240

N-terminal Sequence
Analysis

Leu25

Predicted Molecular Mass

68.6 kDa

SDS-PAGE

110-115 kDa, reducing conditions

Product Datasheets

1095-ER

Product Datasheet

1095-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1-3).

References

Daly, R.J. (1999) Growth Factors, 16:255.
Schlessinger, J. (2000) Cell. 103:211.
Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Related Research Areas

Citations for Recombinant Human EGFR Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

A Platform To Enhance Quantitative Single Molecule Localization Microscopy
Authors: O Golfetto, DL Wakefield, EE Cacao, KN Avery, V Kenyon, R Jorand, SJ Tobin, S Biswas, J Gutierrez, R Clinton, Y Ma, DA Horne, JC Williams, T Jovanovi?-
J. Am. Chem. Soc., 2018-09-26;0(0):.
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance
Impact of Cell-surface Antigen Expression on Target Engagement and Function of an Epidermal Growth Factor Receptor x c-MET Bispecific Antibody.
Authors: Jarantow S, Bushey B, Pardinas J, Boakye K, Lacy E, Sanders R, Sepulveda M, Moores S, Chiu M
J Biol Chem, 2015-08-10;290(41):24689-704.
Applications: Surface Plasmon Resonance
Screening and discovery of nitro-benzoxadiazole compounds activating epidermal growth factor receptor (EGFR) in cancer cells.
Authors: Sakanyan V, Angelini M, Le Bechec M, Lecocq M, Benaiteau F, Rousseau B, Gyulkhandanyan A, Gyulkhandanyan L, Loge C, Reiter E, Roussakis C, Fleury F
Sci Rep, 2014-02-05;4(0):3977.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
Authors: Li N, Nguyen HH, Byrom M, Ellington AD
PLoS ONE, 2011-06-08;6(6):e20299.
Species: Human
Sample Types: RNA
Applications: Affinity Purification, Binding Assay
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Applications: ELISA (Standard)