Home / EGFR / Recombinant Human EGFR Isoform vIII Protein, CF

Recombinant Human EGFR Isoform vIII Protein, CF

Catalog #: 9565-ER
2 Citations Datasheet
Catalog # Availability Size / Price Qty
9565-ER-050
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human EGFR Isoform vIII Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human EGFR protein
Leu25-Ser378 (Asn273Lys), with a C-terminal 6-His tag
Accession #
NP_001333870
N-terminal Sequence
Analysis
Leu25
Predicted Molecular Mass
39 kDa
SDS-PAGE
65 - 80 kDa, reducing conditions

Product Datasheets

You must select a language.

x

9565-ER

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9565-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain (1-3). Human EGFR shares 90% identity with mouse and rat EGFR. The amino acid 25-378 of EGFR encodes the most common extracellular domain truncation mutant, termed EGFRvIII. It has been proved that EGFRvIII is relevant in a number of cancer types. EGFRvIII is tumor specific, and has not been found in normal tissues. EGFRvIII is highly tumorigenic, and can cause aggressive tumor phenotype, with increased invasion, proliferation, angiogenesis, and evasion of apoptosis. This EGFRvIII-mediated enhanced tumourigenicity combined with the lack of EGFRvIII expression in normal tissue makes it an ideal therapeutic targets (4).

References
  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
  4. Kuan, C. T. et al. (2001) Endocrine-related cancer 8:83.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Citations for Recombinant Human EGFR Isoform vIII Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Heterodimerization of T cell engaging bispecific antibodies to enhance specificity against pancreatic ductal adenocarcinoma
    Authors: Long, AW;Xu, H;Santich, BH;Guo, H;Hoseini, SS;de Stanchina, E;Cheung, NV;
    Journal of hematology & oncology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  2. Visualizing Spatial and Stoichiometric Barriers to Bispecific T-cell Engager Efficacy
    Authors: R You, J Artichoker, A Ray, H Gonzalez V, DA Rock, KP Conner, MF Krummel
    Cancer Immunology Research, 2022-06-03;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human EGFR Isoform vIII Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human EGFR Isoform vIII Protein, CF and earn rewards!

Have you used Recombinant Human EGFR Isoform vIII Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review