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Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Catalog #: 142-DC
11 Citations Datasheet
Catalog # Availability Size / Price Qty
142-DC-100
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Citations (11)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED50 for this effect is 0.03‑0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DcR3/TNFRSF6B protein
Human DcR3
(Val 24-His300)
Accession # O95407		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
O95407
N-terminal Sequence
Analysis
Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
57 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

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142-DC

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

142-DC

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IAP Inhibitors

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

References
  1. Pitti, R.M. et al. (1998) Nature 396:699.
  2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
  3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.
Long Name
Decoy Receptor 3
Entrez Gene IDs
8771 (Human); 107126607 (Cynomolgus Monkey)
Alternate Names
DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy

Citations for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
    Authors: A Zwolak, SR Chan, P Harvilla, S Mahady, AA Armstrong, L Luistro, N Tamot, D Yamada, M Derebe, S Pomerantz, M Chiu, R Ganesan, P Chowdhury
    Scientific Reports, 2022-11-29;12(1):20538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1alpha/IL-1 Receptor Antagonist Pathway
    Authors: YJ Peng, CT Peng, YH Lin, GJ Lin, SH Huang, SJ Chen, HK Sytwu, CP Cheng
    Mediators Inflamm., 2020-06-10;2020(0):1237281.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells
    Authors: M Idelson, R Alper, A Obolensky, N Yachimovic, J Rachmilewi, A Ejzenberg, E Beider, E Banin, B Reubinoff
    Stem Cell Reports, 2018-08-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  5. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012-05-14;12(0):176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010-05-01;28(5):939-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy.
    Authors: Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Tsunoda S, Tsutsumi Y, Mukai Y, Okada N, Nakagawa S
    Biomaterials, 2010-02-01;31(12):3357-63.
    Applications: Surface Plasmon Resonance
  8. Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
    Authors: Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
    Leukemia, 2009-07-09;23(11):2139-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid.
    Authors: Mueller AM, Pedre X, Killian S, David M, Steinbrecher A
    J. Neuroimmunol., 2009-03-06;209(1):57-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
    Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
    J. Immunol., 2007-01-01;178(1):192-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006-05-01;54(5):1451-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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