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Recombinant Human CXCL12/SDF-1 gamma Protein

Catalog #: 6448-SD
6 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
6448-SD-025/CF

With Carrier

Catalog # Availability Size / Price Qty
6448-SD-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CXCL12/SDF-1 gamma Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4.

The ED50 for this effect is 4-24 ng/mL.

Source
E. coli-derived human CXCL12/SDF-1 gamma protein
L
Human CXCL12
(Lys22-Asn119)
Accession # NP_001029058
N-terminusC-terminus
Accession #
NP_001029058
N-terminal Sequence
Analysis
Leu-Lys22, Lys22 & Pro23
Predicted Molecular Mass
11.6 kDa
SDS-PAGE
14 kDa, reducing conditions

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6448-SD (with carrier)

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6448-SD/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6448-SD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6448-SD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1 gamma

Human CXCL12 is expressed as five isoforms that differ only in the C-terminal tail (1). The gamma  isoform of CXCL12, also known as SDF-1 gamma, is a 12 kDa, heparin-binding member of the CXC (or alpha) family of chemokines (2, 3). Mature SDF-1 molecules are not glycosylated and exhibit a typical three antiparallel beta -strand chemokine-like fold. N-terminal aa 1 ‑ 8 form a receptor binding site, while aa 1 and 2 (Lys‑Pro) are involved in receptor activation (4). All SDF-1 isoforms can undergo proteolytic processing of the first two N-terminal amino acids by CD26, which is thought to create a reduced‑activity chemokine (5). Human SDF-1 gamma is synthesized as a 119 amino acid (aa) precursor that contains a 21 aa signal sequence and a 98 aa mature region (1). Mature human SDF-1 gamma shares 99%, 97% and 98% aa identity with mouse, rat, and equine SDF-1 gamma, respectively. The unique C‑terminal 26 aa of SDF-1 gamma  are highly charged, including four BBXB (where B = basic and X = any aa) motifs, while the most prevalent form, SDF-1 alpha, has 4 unique C‑terminal aa and binds heparin via the shared BBXB site more N‑terminally located (2, 6). The SDF‑1 gamma C‑terminus binds heparin in secreted SDF-1 gamma, or targets the isoform to the nucleolus in the absence of a signal sequence (6 ‑ 8). SDF-1 isoforms interact with CXCR4 and CXCR7 receptors on the cell surface, and can also bind syndecan-4 (9 ‑ 12). SDF-1 alpha or beta are known to influence lymphopoiesis, enhance the survival of myeloid progenitor cells, regulate the patterning and cell number of neural progenitors, and promote angiogenesis (2). Of all SDF-1 isoforms, SDF-1 gamma is the most strongly attached to the cell surface via glycans, least likely to circulate, and most active in binding CXCR4 and blocking HIV entry to cells via CXCR4 (9, 13). Unlike other isoforms, it is constitutively expressed mainly in the heart or rodent brain, and is not expressed prenatally (14 ‑ 16).

References
  1. Yu, L. et al. (2006) Gene 374:174.
  2. Janowski, M. (2009) Cell Adh. Migr. 3:243.
  3. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  4. Crump, M.P. et al. (1997) EMBO J. 16:6996.
  5. De La Luz Sierra, M. et al. (2004) Blood 103:2452.
  6. Rueda, P. et al. (2007) PLoS ONE 7:e2543.
  7. Laguri, C. et al. (2007) PLoS ONE 10:e1110.
  8. Torres, R. and J.C. Ramirez (2009) PLoS ONE 10:e7570.
  9. Altenburg, J.D. et al. (2010) J. Virol. 84:2563.
  10. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
  11. Levoye, A. et al. (2009) Blood 113:6085.
  12. Charnaux, N. et al. (2005) FEBS J. 272:1937.
  13. Altenburg, J.D. et al. (2007) J. Virol. 81:8140.
  14. Gleichmann, M. et al. (2000) Eur. J. Neurosci. 12:1857.
  15. Segret, A. et al. (2007) J. Histochem. Cytochem. 55:141.
  16. Franco, D. et al. (2009) Anat. Rec. (Hoboken) 292:891.
Long Name
CXCL12/Stromal cell-Derived Factor 1
Entrez Gene IDs
6387 (Human)
Alternate Names
CXCL12/SDF-1 gamma; SDF1 gamma; SDF1g

Citations for Recombinant Human CXCL12/SDF-1 gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Targeting CXCR4 impaired T regulatory function through PTEN in renal cancer patients
    Authors: Santagata, S;Rea, G;Bello, AM;Capiluongo, A;Napolitano, M;Desicato, S;Fragale, A;D'Alterio, C;Trotta, AM;Ieranò, C;Portella, L;Persico, F;Di Napoli, M;Di Maro, S;Feroce, F;Azzaro, R;Gabriele, L;Longo, N;Pignata, S;Perdonà, S;Scala, S;
    British journal of cancer
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Endothelial Autocrine Signaling through CXCL12/CXCR4/FoxM1 Axis Contributes to Severe Pulmonary Arterial Hypertension
    Authors: D Yi, B Liu, T Wang, Q Liao, MM Zhu, YY Zhao, Z Dai
    International Journal of Molecular Sciences, 2021-03-20;22(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane
    Authors: M Kalxdorf, I Günthner, I Becher, N Kurzawa, S Knecht, MM Savitski, HC Eberl, M Bantscheff
    Nature methods, 2021-01-04;18(1):84-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Novel Anti-Inflammatory Peptides Based on Chemokine-Glycosaminoglycan Interactions Reduce Leukocyte Migration and Disease Severity in a Model of Rheumatoid Arthritis
    Authors: EF McNaughton, AD Eustace, S King, RB Sessions, A Kay, M Farris, R Broadbridg, O Kehoe, AJ Kungl, J Middleton
    J. Immunol., 2018-03-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Role of LPAR3, PKC and EGFR in LPA-induced cell migration in oral squamous carcinoma cells.
    Authors: Brusevold I, Tveteraas I, Aasrum M, Odegard J, Sandnes D, Christoffersen T
    BMC Cancer, 2014-06-13;14(0):432.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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