Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

Catalog #: AVI365 Datasheet

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI365-050

Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED₅₀ for this effect is 20-100 ng/mL. Measured by its binding ability in a functional ELISA.

When Recombinant Human CX3CL1/Fractalkine monoclonal antibody (Catalog # MAB3651) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with ED₅₀ of 0.15-0.9 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CX3CL1/Fractalkine protein

Human CX3CL1/Fractalkine (Gln25-Thr338) Accession # P78423.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P78423.1

N-terminal Sequence
Analysis

Gln25, inferred from enzymatic pyroglutamate treatment revealing His26

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

36 kDa

SDS-PAGE

88-103 kDa, under reducing conditions

Product Datasheets

AVI365

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI365

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human CX3CL1/Fractalkine MAb MAB3651) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with an ED₅₀ of 0.15-0.9 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag Protein (Catalog # AVI365) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-103;kDa.

Background: CX3CL1/Fractalkine

Fractalkine, also known as CX3C motif chemokine 1, CX3CL1, neurotactin and small-inducible cytokine D1, is the only member of the CX3C subfamily of the chemokine superfamily (1). Mature human Fractalkine consists of an N-terminal chemokine domain with a CX3C motif and a mucin-like stalk region in the extracellular domain (ECD), a transmembrane segment and a short cytoplasmic domain (1, 2). The soluble form of Fractalkine is generated via ADAM10 and ADAM17 cleavage (1). Within the ECD, human Fractalkine shares 59% amino acid sequence identity with both mouse and rat Fractalkine. Fractalkine exists as both a membrane-bound adhesion molecule and as a soluble proinflammatory chemoattractant and anti-inflammatory neuroprotective agent (1-3). The expression of CX3CL1 is higher in spinal metastases from kidney cancer (4). The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes (5). Most of the functions of CX3CL1 are exerted through the CX#CL1/CX3CR1 axis which has the therapeutic prospect (5, 6). Our Avi-tag Biotinylated Recombinant Fractalkine features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Poniatowski, L. et al. (2017) Mol. Neurobiol. 54:2167.
Desforges, N. et al. (2012) Int. J. Alzheimers Dis. 2012:345472.
Nanki, T. et al. (2016) Mod. Rheumatol. 27:392.
Liu, W. et al. (2016) Arch Immunol. Ther. Exp. (Warsz) 64:371.
Zlotnik, A. Yoshie, O. (2012) Immunity 36:705.
Quan, Z. et al. (2017) Current Gene Therapy 17:442.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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