Home / CX3CL1/Fractalkine / Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

Catalog #: 365-FR
21 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
365-FR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
365-FR-025
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Product Details
Citations (21)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CX3CL1/Fractalkine (Full Length) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 20-100 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CX3CR1. The ED50 for this effect is 2.5-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CX3CL1/Fractalkine protein
Human CX3CL 1
(Gln25 - Arg339) (Ser199Asn)
Accession # P78423		 KQN 6-His tag
N-terminus C-terminus
Accession #
P78423
N-terminal Sequence
Analysis
No results obtained: Gln25 predicted
Predicted Molecular Mass
35 kDa
SDS-PAGE
90 kDa, reducing conditions

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365-FR (with carrier)

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365-FR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

365-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

365-FR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.

References
  1. Pan, Y. et al. (1997) Nature 387:611.
  2. Bazan J.F. et al. (1997) Nature 385:640.
  3. Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer's disease
    Authors: Haage, V;Tuddenham, JF;Comandante-Lou, N;Bautista, A;Monzel, A;Chiu, R;Fujita, M;Garcia, FG;Bhattarai, P;Patel, R;Buonfiglioli, A;Idiarte, J;Herman, M;Rinderspacher, A;Mela, A;Zhao, W;Argenziano, MG;Furnari, JL;Banu, MA;Landry, DW;Bruce, JN;Canoll, P;Zhang, Y;Nuriel, T;Kizil, C;Sproul, AA;de Witte, LD;Sims, PA;Menon, V;Picard, M;De Jager, PL;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 2021-11-14;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CX3CL1/fractalkine regulates the differentiation of human peripheral blood monocytes and monocyte-derived dendritic cells into osteoclasts
    Authors: S Muraoka, K Kaneko, K Motomura, J Nishio, T Nanki
    Cytokine, 2021-07-26;146(0):155652.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD
    Authors: K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch
    International Journal of Molecular Sciences, 2021-07-02;22(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
    Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
    Nature Communications, 2021-02-17;12(1):1087.
    Species: Human
    Sample Types: Reference Standard
  6. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment
    Authors: K Saito, H Iioka, S Maruyama, IW Sumardika, M Sakaguchi, E Kondo
    Neoplasia, 2019-11-20;21(12):1121-1132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The homozygous CX3CR1-M280 mutation impairs human monocyte survival
    Authors: AL Collar, M Swamydas, M O'Hayre, MS Sajib, KW Hoffman, SP Singh, A Mourad, MD Johnson, EM Ferre, JM Farber, JK Lim, CM Mikelis, JS Gutkind, MS Lionakis
    JCI Insight, 2018-02-08;3(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Absence of CX3CR1 impairs the internalization of Tau by microglia
    Authors: M Bolós, M Llorens-Ma, JR Perea, J Jurado-Arj, A Rábano, F Hernández, J Avila
    Mol Neurodegener, 2017-08-15;12(1):59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014-09-24;9(9):e107920.
    Species: Bacteria, Human
    Sample Types: Protein, Whole Cells
    Applications: Enzyme Assay, Enzyme Assay Substrate
  10. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
    Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
    Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
    Blood, 2008-09-17;112(13):5026-36.
    Applications: Western Blot
  13. Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain.
    Authors: Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, Combadiere C, Deterre P
    J. Biol. Chem., 2008-08-25;283(44):30225-34.
    Applications: Western Blot
  14. Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin.
    Authors: Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu XY, Rottoli D, Locatelli M, Buelli S, Pezzotta A, Mapelli P, Geelen J, Remuzzi G, Hawiger J
    J. Immunol., 2008-07-15;181(2):1460-9.
    Applications: Western Blot
  15. Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration.
    Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T
    J. Leukoc. Biol., 2008-04-24;84(1):215-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis.
    Authors: You JJ, Yang CH, Huang JS, Chen MS, Yang CM
    Invest. Ophthalmol. Vis. Sci., 2007-11-01;48(11):5290-8.
    Species: Bovine, Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
    Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
    J. Immunol., 2007-06-15;178(12):8064-72.
    Applications: ELISA (Standard)
  18. Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells.
    Authors: Bolovan-Fritts CA, Trout RN, Spector SA
    J. Virol., 2004-12-01;78(23):13173-81.
    Applications: Western Blot
  19. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Fractalkine mediates natural killer-dependent antitumor responses in vivo.
    Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
    Cancer Res., 2003-11-01;63(21):7468-74.
    Applications: ELISA (Standard)
  21. Detection of fractalkine in human seminal plasma and its role in infertile patients.
    Authors: Zhang Q, Shimoya K, Ohta Y, Chin R, Tenma K, Isaka S, Nakamura H, Koyama M, Azuma C, Murata Y
    Hum. Reprod., 2002-06-01;17(6):1560-4.
    Applications: Western Blot

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