Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF
Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF Summary
Product Specifications
This complex consists of an N-terminal 10-His tagged, neddylated Cullin-4A, untagged RBX1, untagged DDB1, and untagged CRBN.
Product Datasheets
E3-651
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-651
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CUL4A/RBX1/DDB1/CRBN Complex
Cullin-4A is a core component of multiple cullin-RING type ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes. In the DCX complex (DDB1-Cul4-x-box), Cullin-4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the Rbx1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on the neddylation of the cullin subunit, though neddylation may be dispensable in some in vitro reactions.
- Fischer, E.S., et al. (2014) Nature 512:49.
- He, Y.J., et al. (2006) Genes Dev. 20:2949.
- Ito, T., et al. (2010) Science 327:1345.
- Wang, H., et al. (2006) Mol. Cell 22:383.
FAQs
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What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)?
The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. CUL4A is neddylated, or attached to NEDD8. Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated.
For this complex, neddylation is required for ligase activity in vivo, but generally not for in vitro activity. For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650.
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